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Kaempferol compound and its extraction method

An extraction method and compound technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of insufficient research and achieve the effect of simple extraction method and high extraction purity

Active Publication Date: 2020-08-11
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, not enough research has been done on the specific components in the extract

Method used

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  • Kaempferol compound and its extraction method
  • Kaempferol compound and its extraction method
  • Kaempferol compound and its extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The tea fruit of Nanshan tea is dried in the air, the shell is removed, and the cake produced after the oil is removed by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea tea cake. Ultrasonic extraction with 50% ethanol aqueous solution at 60°C for 3 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), and then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 20 times with water to obtain a sample liquid.

[0054] Shelling and degreasing are to maximize the extraction yield and reduce interference during the separation process. Appropriate raw material handling methods are also beneficial to avoid the loss of target compounds.

[0055] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in ...

Embodiment 2

[0061] The tea fruit of Nanshan tea is dried in the air, the shell is removed, and the cake produced after the oil is removed by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea tea cake. Ultrasonic leaching with 60% ethanol aqueous solution at 70°C twice, each time for 2 hours, with a solid-liquid ratio of 1:2 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 22 times with water to obtain a sample liquid.

[0062] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of B...

Embodiment 3

[0067] The tea fruit of Nanshan tea is dried in the air, the shell is removed, and the cake produced after the oil is removed by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea tea cake. Ultrasonic leaching with 55% ethanol aqueous solution at 65°C for 4 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 18 times with water to obtain a sample liquid.

[0068] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of...

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Abstract

The invention provides kaempferol compounds and an extracting method thereof. The extracting method includes: subjecting tea cakes of Camellia semiserrata to leaching, concentrating, extracting, and diluting to obtain a sample liquid; wetly loading the sample liquid, performing gradient eluting, collecting elution, removing ethanol, using a C18ME chromatographic column and methanol as a mobile phase to perform DAC (dynamic axial compression) enrichment, receiving outflow, concentrating in vacuum until no liquid is spun out, dissolving with methanol and acetone to obtain a coarse liquid component; wetly loading the coarse liquid component to a polyamide resin chromatographic column, performing gradient eluting, collecting elution, concentrating, and lyophilizing to obtain crude extract; dissolving the crude extract with a solvent, injecting the sample, and performing DAC to obtain coarse separate; dissolving the coarse separate with a solvent, injecting the sample, and performing DAC toobtain pre-separate; dissolving the pre-separate with a solvent, injecting the sample, and performing gradient eluting to obtain target compounds. The method provided herein helps separate the targetcompounds from nature for the first time, and extraction purity is high.

Description

technical field [0001] The invention relates to the field of compound extraction, in particular to a kaempferol compound and an extraction method thereof. Background technique [0002] Nanshan tea, also known as Guangning safflower oil tea (Camellia semiserrata Chi.), belongs to the plant of the genus Camellia (Camellia L.) of the family Theaceae (Theaceae), and is a perennial tree or shrub. Nanshan tea is mainly distributed in the southeast of Guangxi and the west of Guangdong in China. It is an important cultivated species of camellia oleifera. After research and comparison, it is found that among all kinds of Camellia oleifera, the chemical components in Guangning safflower Camellia oleifera are relatively rich. [0003] Nanshan tea and its cakes are especially rich in compounds such as flavonoids and saponins with various biological activities. At present, the research on Camellia oleifera cake mainly focuses on the optimization of various extraction processes based o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H17/07
CPCC07H1/08C07H17/07
Inventor 曹清明胡丹丹任超劳晓岚何拉丽
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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