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Ganoderma lucidum liquid fermentation technology, fermented product thereof, and applications of fermented product

A technology for liquid fermentation and fermented products, applied in the application, food ingredients, functions of food ingredients, etc., can solve problems such as lack of original innovation, achieve strong anti-superoxide anion free radical activity, enhance efficacy, and avoid bacterial contamination. Effect

Inactive Publication Date: 2019-01-29
广东中测食品化妆品安全评价中心有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Over the years, most of the liquid fermentation of Ganoderma lucidum has adopted this technology, lacking original innovation

Method used

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  • Ganoderma lucidum liquid fermentation technology, fermented product thereof, and applications of fermented product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Prepare Ganoderma lucidum fermentation broth and Ganoderma lucidum mycelium, the steps are as follows:

[0052] (1) Preparation of the extract of Snake-toothed grape: Take 20g of dried sample of Snake-toothed grape, add 400mL of purified water, extract in a water bath at 85°C for 2 hours, extract once, and filter while hot to obtain the extract, which is equivalent to every 20mL of extraction The solution contains 1g of raw material;

[0053] (2) Take preserved strains of Ganoderma lucidum (purchased from the Culture Collection Center of Guangdong Institute of Microbiology, the strain number is GDMCC5.250), activate and culture at 26°C, transfer to test tube PDA slant solid medium, and grow into dense mycelia Robust, pure white slanted species, reserved;

[0054] The formula of the slant medium is: peeled potato 200g, glucose 20g, peptone 2g, (NH4) 2 SO 4 2g, MgSO 4 ·7H 2 O 1g, KH 2 PO 4 1g, 20g agar, add distilled water to 1000mL, adjust pH to 6.5;

[0055] (...

Embodiment 2

[0063] Preparation of ganoderma lucidum fermented liquid and ganoderma mycelium added with Snake dentata grape, the steps are as follows:

[0064] (1) Preparation of the extract of Snake-toothed grape: Take 20g of dried sample of Snake-toothed grape, add 400mL of purified water, extract in a water bath at 85°C for 2 hours, extract once, and filter while hot to obtain the extract, which is equivalent to every 20mL of extraction The solution contains 1g of raw material;

[0065] (2) Take preserved strains of Ganoderma lucidum (purchased from the Culture Collection Center of Guangdong Institute of Microbiology, the strain number is GDMCC5.250), activated at 26°C and transferred to the inclined surface of the test tube PDA to grow dense and strong hyphae with strong color Pure white slanted seeds, spare;

[0066] The formula of the slant medium is: peeled potato 200g, glucose 20g, peptone 2g, (NH4) 2 SO 4 2g, MgSO 4 ·7H 2 O 1g, KH 2 PO 4 1g, 20g agar, add distilled water ...

Embodiment 3

[0075] Preparation of ganoderma lucidum fermented liquid and ganoderma mycelium added with Snake dentata grape, the steps are as follows:

[0076] (1) Preparation of the extract of Snake-toothed grape: Take 20g of dried sample of Snake-toothed grape, add 400mL of purified water, extract in a water bath at 85°C for 2 hours, extract once, and filter while hot to obtain the extract, which is equivalent to every 20mL of extraction The solution contains 1g of raw material;

[0077] (2) Take preserved strains of Ganoderma lucidum (purchased from the Culture Collection Center of Guangdong Institute of Microbiology, the strain number is GDMCC5.250), activated at 26°C and transferred to the inclined surface of the test tube PDA to grow dense and strong hyphae with strong color Pure white slanted seeds, spare;

[0078] The formula of the slant medium is: peeled potato 200g, glucose 20g, peptone 2g, (NH4) 2 SO 4 2g, MgSO 4 ·7H 2 O 1g, KH 2 PO 4 1g, 20g agar, add distilled water ...

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Abstract

The invention discloses a Ganoderma lucidum liquid fermentation technology, a fermented product thereof, and applications of the fermented product. The fermentation technology comprises: preparing anAmpelopsis grossedentata extraction liquid by using a hot extraction method; carrying out activating culture on a Ganoderma lucidum storage train, transferring to a test tube slope solid culture medium, growing to obtain a slope strain with characteristics of dense and strong hypha and white color; picking a plurality of the Ganoderma lucidum strain blocks from the slope, inoculating into a liquidculture medium, and carrying out shake cultivation to prepare a liquid strain; adding the Ampelopsis grossedentata extraction liquid into a base culture medium, mixing to obtain a fermentation full-component culture medium, inoculating the liquid strain, and carrying out deep fermentation; and carrying out solid-liquid separation on the deep fermentation product to obtain the Ganoderma lucidum fermentation broth and the Ganoderma lucidum mycelium. According to the present invention, the efficacy of the Ganoderma lucidum mycelium fermentation product can be enhanced so as to expand the use; the Ganoderma lucidum fermentation broth has strong anti-oxidation effect; and the whole fermentation process avoids the contamination of the bacteria, the fermentation product is safe and non-toxic, and has significant health care effect, high development value and broad application prospect.

Description

【Technical field】 [0001] The invention relates to a ganoderma lucidum liquid fermentation technology and its fermented products and application, belonging to the technical field of biological fermentation. 【Background technique】 [0002] The liquid submerged fermentation of edible and medicinal fungi refers to cultivating the mycelium of edible and medicinal fungi in a liquid medium with comprehensive nutrition, while introducing sterile air and mechanically stirring or shaking, and controlling external conditions such as suitable temperature and pressure. Mycelium grows and reproduces rapidly, so that mycelium and other metabolites can be obtained quickly and efficiently, which can be used in scientific research or industrial production. The liquid culture technology of edible and medicinal fungi has been widely researched and applied due to its advantages such as short production cycle, large output, high quality, and outstanding economic benefits. Fermentation products a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L2/52A23L33/00A23L33/105C12N1/14C12R1/645
CPCA23L2/382A23L2/52A23L33/00A23L33/105C12N1/14A23V2002/00A23V2200/30A23V2250/21
Inventor 张命龙彭密军陈智勇杨秋玲王志宏秦俊莲
Owner 广东中测食品化妆品安全评价中心有限公司
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