The Application of Combination of Loganinin and 5-Fluorouracil in the Treatment of Gastric Cancer
A technology of fluorouracil and loganinin, which is applied in the field of tumor biotherapy and can solve problems such as large side effects and poor therapeutic effect
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Embodiment 1
[0033] Example 1. The effect of loganinin combined with 5-fluorouracil on the growth and proliferation of HGC27 and MGC803 cells was detected by MTT assay.
[0034] Thiazolium blue (MTT) is a yellow dye, which can dissolve formazan by using its characteristic that it only reacts with the dehydrogenase of living cells to generate blue-purple crystals (formazan) and deposits in cells, but does not react with dead cells. In DMSO, the ratio of living cells can be reflected by measuring the absorbance value at a specific wavelength with an enzyme-linked immunosorbent assay. The inventor made HGC27 and MGC803 cells into 4×10 4 cells / mL, add 100 μL per well into the 96-well plate culture medium. Divided into 4 groups (control group, 5-fluorouracil group, loganinin group and combination group) administered separately, after adding MTT for 72 hours of color development, the intervention was terminated, the culture medium was discarded, and the concentration of MTT was adjusted to 0.5 ...
Embodiment 2
[0040] Example 2. The effect of loganin combined with 5-fluorouracil on the growth and proliferation of HGC27 and MGC803 cells was detected by colony formation assay.
[0041] The cell clone formation rate is what is commonly referred to as the cell inoculation survival rate. It refers to the number of adherent cells that can survive and develop into clones during the process of adherent growth. It should be pointed out that not all cells that can successfully adhere to the wall can proliferate and grow, thus eventually forming clones. That is to say, cells not only need to successfully adhere to the wall but also have strong proliferative activity to form clones. Therefore, the detection of clone formation rate is one of the indicators to investigate the dependence of cell population and proliferation ability. The inventors inoculated HGC27 and MGC803 cells in 6-well plates according to 500 cells / well, and divided them into 4 groups (control group, 5-fluorouracil group, log...
Embodiment 3
[0043] Example 3. Effects of loganin and 5-fluorouracil on the sphere-forming ability of HGC27 and MGC803 cells
[0044] The present inventor digested and centrifuged the adherent HGC27 and MGC803 cells with trypsin, poured off the supernatant, and sucked up the remaining liquid as much as possible with a pipette tip. Add about 2ml of sterile PBS to resuspend the cells, centrifuge at 800rpm for 2 minutes, discard the PBS, and repeat the above process once to wash away the remaining serum-containing culture medium in the cells. Resuspend the cells with stem cell culture medium to an appropriate concentration, and after counting with a cell counter, inoculate the same number (10,000) cells of the control group and the experimental group into a low-adhesion culture dish to which stem cell culture medium has been added in advance to cultivate. They were divided into 4 groups (control group, 5-fluorouracil group, loganinin group and combination group) and administered respectively...
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