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New secondary metabolite LW-4 of Aspergillus terreus SKL-001

A secondary metabolite, LW-4 technology, applied in the biological field, can solve the problem of not obvious inhibitory activity of Gram-negative bacteria, and achieve the effect of improving the extraction rate of fermentation products and accelerating the release of intracellular substances

Inactive Publication Date: 2019-02-01
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isolated compound has a strong inhibitory effect on methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE), and has a certain inhibitory effect on Gram-negative bacteria. The minimum inhibitory concentration MIC is less than 12.5μM, indicating that it can be developed into a potential antibacterial drug, but the inhibitory activity of the fungal secondary metabolite derivatives from Liushan Lake on Gram-negative bacteria is not obvious

Method used

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  • New secondary metabolite LW-4 of Aspergillus terreus SKL-001
  • New secondary metabolite LW-4 of Aspergillus terreus SKL-001

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Embodiment 1

[0021] A new secondary metabolite LW-4 of Aspergillus terreus SKL-001 is a compound represented by formula I, its stereoisomer or a pharmaceutically accessible salt, and formula I has the following structure:

[0022]

[0023] A method for preparing a new secondary metabolite LW-4 of Aspergillus terreus SKL-001, comprising spore preparation, bacterial strain fermentation, secondary metabolite extraction and separation and purification, the specific steps are:

[0024] Spore preparation: put the glycerin cryopreservation tube of Aspergillus terreus SKL-001 (containing 20% ​​glycerol and 3.3% seawater freshly prepared PDA liquid freezing solution) stored in a -80°C ultra-low temperature refrigerator in a clean operating table, and recover for 10 minutes , Dip the resuscitated cryopreservation solution with an inoculation loop after burning and sterilize, and evenly streak in the freshly prepared PDA slant solid medium, and then place the slant medium in a constant temperature ...

Embodiment 2

[0029] A new secondary metabolite LW-4 of Aspergillus terreus SKL-001, which is a compound represented by formula I, its stereoisomer or a pharmaceutically accessible salt, characterized in that formula I has the following structure:

[0030]

[0031] A method for preparing a new secondary metabolite LW-4 of Aspergillus terreus SKL-001, comprising spore preparation, bacterial strain fermentation, secondary metabolite extraction and separation and purification, the specific steps are:

[0032]Spore preparation: put the glycerin cryopreservation tube of Aspergillus terreus SKL-001 (containing 20% ​​glycerol and 3.3% seawater freshly prepared PDA liquid freezing solution) stored in a -80°C ultra-low temperature refrigerator in a clean operating table, and recover for 10 minutes , Dip the resuscitated cryopreservation solution with an inoculation loop after burning and sterilize, and evenly streak in the freshly prepared PDA slant solid medium, and then place the slant medium in...

Embodiment 3

[0037] A new secondary metabolite LW-4 of Aspergillus terreus SKL-001, which is a compound represented by formula I, its stereoisomer or a pharmaceutically accessible salt, characterized in that formula I has the following structure:

[0038]

[0039] A method for preparing a new secondary metabolite LW-4 of Aspergillus terreus SKL-001, comprising spore preparation, bacterial strain fermentation, secondary metabolite extraction and separation and purification, the specific steps are:

[0040] Spore preparation: put the glycerin cryopreservation tube of Aspergillus terreus SKL-001 (containing 20% ​​glycerol and 3.3% seawater freshly prepared PDA liquid freezing solution) stored in a -80°C ultra-low temperature refrigerator in a clean operating table, and recover for 10 minutes , Dip the resuscitated cryopreservation solution with an inoculation loop after burning and sterilize, and evenly streak in the freshly prepared PDA slant solid medium, and then place the slant medium i...

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Abstract

The invention discloses a new secondary metabolite LW-4 of Aspergillus terreus SKL-001, wherein the new secondary metabolite LW-4 has the following structure defined in the specification, wherein R1 and R2 respectively and independently are H, OH, C1-C4 alkoxy, C1-C4 alkylsulfonyl and C2-C4 alkyl acyl, one of R1 and R2 is H, R3 and R4 respectively and independently are H, OH, halogen, amino, a group defined in the specification, C1-C4 alkyl, C2-C4 alkyl acyl, C1-C4 haloalkyl, C1-C4 alkylsulfonyl and C2-C4 alkenyl, and at least one of R3 and R4 is not H. The preparation method comprises: addingan epigenetic modification regulator SAHA, paricalcitol and triglyceride to a culture medium, and carrying out standing fermentation culture. According to the present invention, Aspergillus terreus SKL-001 is subjected to epigenetic modification by adding the epigenetic modification regulator SAHA to the culture medium to promote the production of the diversified secondary metabolites, such thatthe new secondary metabolite LW-4 is separated; and the new secondary metabolite LW-4 of the Aspergillus terreus SKL-001, and the stereoisomer or pharmaceutically contactable salt thereof have long-acting antibacterial and anti-inflammatory effects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new secondary metabolite LW-4 of Aspergillus terreus SKL-001. Background technique [0002] As we all know, microorganisms have long been an important source of active substances such as antibiotics and enzyme inhibitors. Microorganisms have become a hotspot in the field of natural product research due to their large-scale fermentation and cultivation, no raw material restrictions, and no environmental damage. In recent years, the probability of obtaining new compounds from terrestrial microorganisms has been greatly reduced. However, due to the specific living environment of marine-derived microorganisms, in addition to producing metabolites similar to those on land, sometimes some new compounds with specific structures can also be produced, showing a huge development and utilization prospects. Aspergillus fungi have always been an important species of natural product research, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04C12P17/18A61P31/04C12R1/66
CPCA61P31/04C07D487/04C12P17/182
Inventor 孙坤来张译文王斌初学梅陈荫赵玉勤
Owner ZHEJIANG OCEAN UNIV
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