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Method for extracting collagen protein from tuna skin via enzymolysis assisted by impulse ultrasound

A technology of pulsed ultrasound and tuna, applied in the field of natural protein extraction, to achieve the effect of improving the extraction rate, simple and easy operation process, and strengthening the reaction

Inactive Publication Date: 2019-02-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, continuous ultrasound is generally used to promote the extraction of tuna collagen by ultrasound. However, according to the current research literature, no advanced pulse ultrasound mode has been applied to the preparation of tuna skin collagen.

Method used

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  • Method for extracting collagen protein from tuna skin via enzymolysis assisted by impulse ultrasound
  • Method for extracting collagen protein from tuna skin via enzymolysis assisted by impulse ultrasound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of the enzyme-extracted tuna skin collagen without ultrasonic treatment is carried out according to the following steps:

[0024] (1) The fish skin is thawed at 4°C, washed, drained, chopped, degreased and decontaminated, fully washed with distilled water until neutral, dried at low temperature, and set aside.

[0025] (2) Soak the fish skin raw material treated in step (1) in a 0.5M acetic acid solution with a solid-liquid ratio of 1:200 (mg / mL), homogenize it in an ice bath for 5 minutes, and add 0.06% (W / V) 1800U / g of pepsin (pH=2), 48 hours of enzymatic extraction at 4°C, centrifuged at 8000rpm at 4°C for 45min, the supernatant is the crude collagen extract without sonication.

[0026] (3) Add NaCl to the crude extract prepared in step (2) to 0.9M for salting out overnight, centrifuge at 6000rpm for 20min, dissolve the resulting precipitate in 0.5M acetic acid, and centrifuge at 8000rpm for 45min, add NaCl to the resulting supernatant to reac...

Embodiment 2

[0033] The preparation method of enzyme-extracted tuna skin collagen treated with different ultrasonic powers is carried out according to the following steps:

[0034] (1) The fish skin is thawed at 4°C, washed, drained, chopped, degreased and decontaminated, fully washed with distilled water until neutral, dried at low temperature, and set aside.

[0035] (2) Soak the fish skin raw material treated in step (1) in 0.5M acetic acid solution with a material-to-liquid ratio of 1:200 (mg / mL), homogenize in an ice bath for 5 minutes, and perform pulse ultrasonic treatment. Ultrasonic treatment parameters It is: frequency 28kHz; duty cycle 50% (5s / 5s); time 40min; power 100W. Add 0.06% (W / V) 1800U / g pepsin (pH = 2) after the ultrasonication, 48 hours of enzyme extraction at 4°C, and centrifuge at 8000rpm at 4°C for 45min, the supernatant is the crude collagen extracted by ultrasonic-assisted enzymatic extraction Extraction.

[0036] (3) Add NaCl to the crude extract prepared in st...

Embodiment 3

[0043] The ultrasonic power of the ultrasonic treatment is 200W, and the rest of the steps are the same as in Example 2; the specific results of the collagen extraction rate are shown in Table 1.

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Abstract

The invention discloses a method for extracting collagen protein from tuna skin via enzymolysis assisted by impulse ultrasound, which belongs to the technical field of natural protein extraction. Themethod comprises the following steps: firstly, the tuna skin is treated for defatting and removing miscellaneous proteins; secondly, an impulse ultrasound assistance method is adopted for speeding upthe extraction process in which pepsin is adopted for the enzymolysis of the collagen protein; and finally, the tuna skin collagen protein with a I type spiral structure is obtained by centrifugation,purification and drying after the enzymolysis assisted by the impulse ultrasound is finished. By adopting the enzymolysis assisted by the impulse ultrasound for speeding up the extraction process ofthe collagen protein and finally obtaining the tuna skin collagen protein with the I type spiral structure, the invention has the advantages that the operation process is simple and easy, the extraction rate of the tuna skin collagen protein is greatly improved, and the energy consumption is lower.

Description

technical field [0001] The invention relates to the technical field of natural protein extraction, in particular to a method for extracting collagen by using tuna skin as a raw material and adopting pulse ultrasonic-assisted enzymatic hydrolysis. Background technique [0002] At present, with the increase of tuna consumption at home and abroad, the amount of tuna processing is increasing, and processing enterprises produce a large amount of by-products of tuna skin, which have not been fully developed and utilized. As far as the current situation is concerned, most of the leftovers from the processing of aquatic products such as tuna skin are directly discarded, which not only causes serious waste of resources but also pollutes the environment. Therefore, research on methods to make full use of tuna skin resources and develop high value-added products can not only further increase the economic value of tuna, but also effectively reduce resource waste, which is of great signi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14C12P21/06
CPCC07K14/78C12P21/06
Inventor 曲文娟冯依婷张欣欣马海乐
Owner JIANGSU UNIV
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