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Preparation method of cell wall with high glucan content

A cell wall and glucan technology, applied in the field of high-sugar cell wall products, can solve the problem of low utilization rate of yeast cell wall, and achieve the effect of cost-effective and reduced enzyme amount

Inactive Publication Date: 2019-02-15
SHANDONG BIO SUNKEEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is mainly aimed at the low utilization rate of yeast cell walls and the obvious disadvantages of the traditional strong-acid and strong-alkali combination method, and adopts a relatively mild method to obtain yeast cell walls with high glucan content

Method used

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  • Preparation method of cell wall with high glucan content
  • Preparation method of cell wall with high glucan content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The specific process is:

[0025] (1) Slurry: recycle the waste material after yeast extraction to make a slurry with a cell wall content of 10wt%;

[0026] (2) Cell wall activation: add NaOH solution to the cell wall slurry to adjust the pH to 8, use a reactor to heat up to 120°C, and keep warm for 4 hours;

[0027] (3) Protease hydrolysis: cool the activated cell wall slurry to 60°C, adjust the pH to 7.5, add 4.5‰ of cell wall dry matter alkaline protease, and act for 8 hours;

[0028] (4) Cell wall enzymatic hydrolysis: lower the temperature to 48°C, adjust the pH to 5, add 2wt‰ plant hydrolase to the dry matter of the cell wall, and act for 7 hours;

[0029] (5) Enzyme inactivation: heat up to 90°C, keep warm for 60 minutes, then cool down to 65°C; Separation: Use a high-speed disc centrifuge at 5000r / min, centrifuge, and take the heavy phase;

[0030] (6) Washing: wash the separated heavy phase twice, the ratio of material to water is 1:1;

[0031] (7) Powder sp...

Embodiment 2

[0034] (1) Slurry: recycle the waste material after yeast extraction to make a slurry with a cell wall content of 8wt%;

[0035] (2) Cell wall activation: add NaOH solution to the cell wall slurry to adjust the pH to 7.5, use a reactor to heat up to 125°C, and keep warm for 4 hours;

[0036] (3) Protease enzymatic hydrolysis: cool the activated cell wall slurry to 65°C, adjust the pH to 7, add 4‰ alkaline protease of the dry matter mass of the cell wall, and act for 8 hours;

[0037] (4) Cell wall enzymatic hydrolysis: lower the temperature by 50°C, adjust the pH to 5.5, add 1.8‰ of cell wall dry matter plant hydrolase, and act for 8 hours;

[0038] (5) Enzyme inactivation: heat up to 85°C, keep warm for 60 minutes, then cool down to 65°C; Separation: Use a high-speed disc centrifuge at 5000r / min, centrifuge, and take the heavy phase;

[0039] (6) Washing: wash the separated heavy phase twice, the ratio of material to water is 1:1;

[0040] (7) Powder spraying: adjust the so...

Embodiment 3

[0043] (1) Slurry: recycle the waste material after yeast extraction to make a slurry with a cell wall content of 12wt%;

[0044] (2) Cell wall activation: add NaOH solution to the cell wall slurry to adjust the pH to 8.5, use a reactor to heat up to 110°C, and keep warm for 2 hours;

[0045] (3) Protease enzymatic hydrolysis: cool the activated cell wall slurry to 65°C, adjust the pH to 8, add 3‰ of the cell wall dry matter alkaline protease, and act for 10 hours;

[0046] (4) Cell wall enzymatic hydrolysis: lower the temperature to 45°C, adjust the pH to 4.5, add 1.8‰ of cell wall dry matter plant hydrolase, and act for 7 hours;

[0047] (5) Enzyme inactivation: heat up to 85°C, keep warm for 30 minutes, and cool down to 60°C; Separation: Use a high-speed disc centrifuge at 5000r / min, centrifuge, and take the heavy phase;

[0048] (6) Washing: wash the separated heavy phase twice, the ratio of material to water is 1:1;

[0049] (7) Powder spraying: adjust the solid content...

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Abstract

The invention provides a method for preparing a high-glucan cell wall product. The method adopts a flow path shown as a figure 1 in the specification. The cell wall product prepared with the method has a stable glucan content of 40% or above, which is greatly higher than that of the existing product in the market; in addition, a raw material used in the invention is a byproduct obtained during yeast cell extraction, and is low in cost; the cell wall product with high glucan content is widely applied to the fields of feed, food and the like; therefore, the method has a great economic prospect.

Description

technical field [0001] The invention belongs to the technical field of yeast cell wall purification, and in particular relates to a novel mild method for purifying waste yeast cell wall glucan to finally obtain high-sugar cell wall products. Background technique [0002] Yeast cell wall is the main by-product in the production of yeast extract. It is estimated that if 100 tons of extract are produced every year, about 40 tons of waste yeast cell wall (water content is about 80%) can be obtained. The production of yeast cell wall has an important impact on the entire ecological environment The nutritional value of yeast cell wall is relatively rich, containing nutrients such as glucan, mannan, vitamins and trace elements. With the continuous strengthening of resistance reduction, yeast cell wall is increasingly recognized as a high-efficiency immune enhancer, and the market capacity continues to expand. However, the homogenization of cell walls in the market is serious and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0006
Inventor 易勇刘帅牛丹丹张继祥魏艳丽张强刘彪杜显雨
Owner SHANDONG BIO SUNKEEN
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