Collagen-based self-healing hydrogel and preparation method thereof

A self-healing and hydrogel technology, applied in the field of collagen materials, can solve the problems of non-self-healing, difficult to self-repair, shorten the life of hydrogel implants, etc., to achieve a good cell living environment, good toughness and self-healing sexual effect

Active Publication Date: 2019-02-15
FUJIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the collagen hydrogel prepared by physical method, due to the weak intermolecular force, it is easy to be destroyed when subjected to external force; while the hydrogel formed by covalent bond cross-linking has a stable network structure and makes the gel The mechanical properties are improved, but at the same time, it also restricts the fluidity of the molecular cha

Method used

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  • Collagen-based self-healing hydrogel and preparation method thereof

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Example Embodiment

[0030] A preparation method of collagen-based self-repairing hydrogel includes the following specific steps:

[0031] (1) Dissolve collagen in an acetic acid solution with a concentration of 0.01~0.1 mol / L, and then stir it evenly to obtain a collagen solution with a mass concentration of 10~30 mg / mL;

[0032] (2) According to the ratio of guar gum and dialdehyde guar gum mass ratio and concentration of 0.5~4:1, respectively pipette guar gum and dialdehyde guar gum and dissolve them in deionized water at the same time, and then mix them in 30~80 Stir and mix for 2-6 hours at a temperature of ℃ to obtain a guar gum / dialdehyde guar gum compound solution with a total mass concentration of 10-30 mg / mL;

[0033] (3) Dissolve borax in PBS buffer to prepare a borax solution with a mass concentration of 0.05 to 0.2 mol / L;

[0034] (4) Blend the collagen solution prepared in step (1) with equal mass concentration and the guar gum / dialdehyde guar gum compound solution prepared in step (2) under...

Example Embodiment

[0043] Example 1

[0044] (1) Dissolve 5g guar gum in 50 mL ethanol and add 40 mL NaIO with a concentration of 0.1 mol / L 4 After the oxidation reaction is completed, add 0.5 mL of ethylene glycol solution to the mixed solution to terminate the reaction, and stir for 0.5 h at room temperature; wash the reaction product 3 times with tert-butanol, After cleaning, it was subjected to vacuum freezing treatment for 12 h, and then the product obtained from the treatment was ground to obtain powdered aldehyde-based guar gum with a degree of hydroformylation of 4%.

[0045] (2) Cut the cowhide into small pieces, put it in a meat grinder, and then put the ground cowhide with a wet weight of about 10g into a 1 L conical flask, and add pepsin equivalent to 3% of the wet weight of the cowhide And 500 mL of acetic acid with a concentration of 0.1 mol / L, and then put it in a constant temperature shaker at 4 ℃ for shaking extraction at a speed of 180 r / min for 48 h. The solution after shaking ext...

Example Embodiment

[0050] Example 2

[0051] (1) Dissolve 10 g of guar gum in 50 mL of ethanol and add 40 mL of 0.5 mol / L NaIO 4 After the oxidation reaction is completed, add 2 mL of ethylene glycol solution to the product to stop the reaction, and stir at room temperature for 2 hours; wash the reaction product 5 times with tert-butanol, and then wash it. Carry out vacuum freezing treatment for 24 hours, and then grind the product obtained from the treatment to obtain a powdered aldehyde-based guar gum with a degree of hydroformylation of 8%;

[0052] (2) Cut the cowhide into small pieces, put it in a meat grinder, and put the ground cowhide with a wet weight of about 10 g into a 1 L conical flask, and add pepsin equivalent to 3% of the wet weight of the cowhide And 500 mL of acetic acid with a concentration of 0.5 mol / L, and then put it in a 4 ℃ constant temperature shaker for extraction with shaking at a speed of 180 r / min for 72 h. The solution after shaking extraction was centrifuged, salted ou...

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Abstract

The invention discloses collagen-based self-healing hydrogel and a preparation method thereof. The method comprises the following steps: uniformly mixing a collagen solution and a mixed solution of guar gum and dialdehyde guar gum, adding a borax solution, stirring and mixing, and performing vacuum defoaming, so as to obtain the collagen-based self-healing hydrogel. A feasible approach and a novelthought are provided for the preparation of the collagen-based self-healing hydrogel through the scheme. In addition, the self-healing hydrogel can properly meet the requirement of injectable hydrogel, and structures damaged during injection can be reconstructed in organisms; moreover, the self-healing hydrogel as a three-dimensional cell culture carrier has unique superiorities, can properly simulate cell living environment, is beneficial to transfer of contact information among cells and to realizing of coculture of various and multi-layer cells.

Description

technical field [0001] The invention relates to the technical field of collagen materials, in particular to a collagen-based self-repairing hydrogel and a preparation method thereof. Background technique [0002] Collagen is the most abundant protein in animals, accounting for about one-third of the total protein in animals. Collagen's low antigenicity, good biocompatibility, and biodegradability make it widely used in various fields such as biomedical materials, drug delivery vehicles, tissue engineering, and cosmetics. In recent years, protein hydrogels, as an important class of biomaterials, have been widely used in the fields of biomedicine and tissue engineering. Protein hydrogel is a natural matrix with a three-dimensional network structure composed of protein molecules, water and cross-linking agents. Collagen hydrogels have similar properties to natural tissues and are biodegradable. Related research has attracted more and more researchers' attention, especially ge...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08L5/00C08K3/38C08J3/075C08B37/00
CPCC08B37/0096C08J3/075C08J2389/00C08J2405/00C08K2003/387
Inventor 丁翠翠张敏兰飞龚凌诸郑志功
Owner FUJIAN UNIV OF TECH
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