Extracting method of selenoprotein in pear fruit

An extraction method and selenoprotein technology, which are applied in the field of plant physiology, can solve problems such as the loss of selenoprotein, and achieve the effects of less selenoprotein impurities, good absorption effect and simple production process

Inactive Publication Date: 2019-02-19
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent extraction method has been widely promoted due to its high efficiency and low cost, but at the same time there is also the problem of serious loss of selenoproteins. Therefore, research on a method with high-efficiency extraction of selenoproteins has broad market promotion value

Method used

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  • Extracting method of selenoprotein in pear fruit
  • Extracting method of selenoprotein in pear fruit
  • Extracting method of selenoprotein in pear fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 A kind of extraction method of pear pulp selenoprotein

[0031] Such as figure 1 Shown, a kind of extraction method of pear pulp selenoprotein comprises the following steps:

[0032] 1) Pre-treatment: select Dangshansu pears with uniform fruit size as the test material, wash them with tap water first, then rinse them repeatedly with distilled water, remove the peel and core, keep the pulp, chop and dry it, and grind it into dry powder with a grinder for later use;

[0033] 2) Water extraction: Weigh 5.0g of dry powder sample into a three-necked flask, add 40ml of pure water to the Erlenmeyer flask at room temperature and shake for 1 hour, after fully stirring, add a centrifuge tube and centrifuge at 4000r / min in a centrifuge, and take it out after centrifugation for 15min. Supernatant and residue 1, add 40ml of pure water to residue 1, shake again at room temperature 2 times to obtain supernatant and residue 2, combine 3 times to obtain supernatant, that i...

Embodiment 2

[0039] Embodiment 2 A kind of extraction method of pear pulp selenoprotein

[0040] 1) Pre-treatment: select Dangshansu pears with uniform fruit size as the test material, wash them with tap water first, then rinse them repeatedly with distilled water, remove the peel and core, keep the pulp, chop and dry it, and grind it into dry powder with a grinder for later use;

[0041] 2) Water extraction: Weigh 5.0g of dry powder sample into a three-necked flask, add 100ml of pure water to the conical flask at room temperature and shake for 1 hour, stir well, add a centrifuge tube and centrifuge at 4000r / min in a centrifuge, and take it out after centrifugation for 15min. Supernatant and residue 1, add 100ml of pure water to residue 1, shake twice at room temperature to obtain supernatant and residue 2, combine 3 times to obtain supernatant, that is, water-soluble selenoprotein extract 1, At the same time, weigh the same amount of dry powder after step 1) and repeat the aforementioned ...

Embodiment 3

[0047] Embodiment 3 A kind of extraction method of pear pulp selenoprotein

[0048] 1) Pre-treatment: select Dangshansu pears with uniform fruit size as the test material, wash them with tap water first, then rinse them repeatedly with distilled water, remove the peel and core, keep the pulp, chop and dry it, and grind it into dry powder with a grinder for later use;

[0049] 2) Water extraction: Weigh 5.0g of dry powder sample into a three-necked flask, add 60ml of pure water to the conical flask at room temperature and shake for 1 hour, after fully stirring, add a centrifuge tube and centrifuge at 4000r / min in a centrifuge, and take it out after centrifugation for 15min. Supernatant and residue 1, add 60ml of pure water to residue 1, shake again at room temperature 2 times to obtain supernatant and residue 2, combine 3 times to obtain supernatant, that is, water-soluble selenoprotein extract 1, At the same time, weigh the same amount of dry powder after step 1) and repeat th...

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Abstract

The invention discloses an extracting method of selenoprotein in a pear fruit. The method comprises the steps that pear pulp is taken to be dried and grounded into powder, a mild continuous extractingmethod is adopted, and the continuous extracting method is characterized in that distilled water, a NaCl solution, ethanol and monometallic sodium orthophosphate-monopotassium phosphate buffering liquid are taken as solvents to sequentially perform extraction to obtain pear fruit selenoprotein with different dissolvability. According to the extracting method, milder extracting and stirring extracting manners are adopted to extract pear pulp selenoprotein, the yield is high, extraction is conducted through ultrasound and mechanical vibration, the recovery rate is lower, water-solubility selenoprotein, salt-solubility selenoprotein and alcohol-solubility selenoprotein are extracted under the mild extraction taking the distilled water, the NaCl solution, the ethanol and the monometallic sodium orthophosphate-monopotassium phosphate buffering liquid as the solvents, and the method capable of efficiently extracting selenium polysaccharide has wider marketing popularization value.

Description

technical field [0001] The invention belongs to the field of plant physiology, and in particular relates to a method for extracting selenoproteins in pear fruits. Background technique [0002] Selenium is an essential trace element for organisms, and the selenium ingested by humans or animals comes directly or indirectly from plants. Selenium is recognized as the "King of Anticancer" in the world, and it is also one of the indispensable trace elements for the human body. It has anti-oxidation effect, because many diseases such as Keshan disease, atherosclerosis, and cataract formation are related to active free radicals. For example, it can work together with vitamin E to prevent the attack of oxygen free radicals on cell membranes, so it can supplement A certain amount of selenium can remove free radicals and cholesterol in the human body and prevent the occurrence of chronic diseases. [0003] After selenium is absorbed by plants, it forms a very complex chemical form, w...

Claims

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Application Information

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IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 王纪忠杨美玲靳丛王云鹏潘国庆金蕾
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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