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Silk fibroin-dopamine-E7 oligopeptide compound stent and preparation method and application thereof

A technology of silk fibroin and composite scaffold, which is applied in pharmaceutical formulations, coatings, pharmaceutical sciences, etc., can solve the problem of weak osteoinductive performance of silk protein, accelerate osteogenic differentiation, improve hydrophilicity, and promote cell adhesion. The effect of adhering to proliferation

Active Publication Date: 2019-02-22
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Silk protein is a natural polymer material with no toxic and side effects, but the osteoinductive properties of silk protein itself are weak. In this field, further research on silk protein electrospun scaffolds is needed in order to facilitate its application in the field of bone tissue regeneration.

Method used

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  • Silk fibroin-dopamine-E7 oligopeptide compound stent and preparation method and application thereof
  • Silk fibroin-dopamine-E7 oligopeptide compound stent and preparation method and application thereof
  • Silk fibroin-dopamine-E7 oligopeptide compound stent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Preparation of the scaffold

[0059] First, dissolve 8g silk protein (SF) in 80ml hexafluoroisopropanol mixed solvent to form a 10% (W / V) solution, draw the silk protein mixture into a 10ml injection syringe, and inject 1.5-2ml of it through a micro-injection pump. Propelling at a rate of / h, the inner diameter of the front needle is a stainless steel flat-head dispensing needle of 0.8mm. During the electrospinning process, the voltage was controlled at 15-20kV, and the distance from the needle to the receiver was 15-20cm. The rotating speed of the drum collector was 300-500 times per minute, and the electrospun fibers were collected on aluminum foil paper and dried in vacuum to obtain SF scaffolds.

[0060] Dissolve dopamine in Tris-buffer, and immerse 8g silk protein electrospinning scaffold (SF scaffold) in 20ml dopamine solution (4mg / ml) for 12 hours, and form a polydopamine (PDA) deposition layer after fully oxidizing and self-polymerizing. Get the SF-PDA holder....

Embodiment 2

[0063] Preparation of the scaffold

[0064] The experimental procedure is the same as in Example 1, except that the voltage in the electrospinning process is changed to 20-25kv.

[0065] First, dissolve 8g silk protein (SF) in 80ml hexafluoroisopropanol mixed solvent to form a 10% (W / V) solution, draw the silk protein mixture into a 10ml injection syringe, and inject 1.5-2ml of it through a micro-injection pump. Propelling at a rate of / h, the inner diameter of the front needle is 0.8mm stainless steel flat dispensing needle. During the electrospinning process, the voltage was controlled at 20-25kV, and the distance from the needle to the receiver was 15-20cm. The rotating speed of the drum collector was 300-500 times per minute, and the electrospun fibers were collected on aluminum foil paper and dried in vacuum to obtain SF scaffolds.

[0066] Dissolve dopamine in Tris-buffer, and immerse 8g silk protein electrospinning scaffold (SF scaffold) in 20ml dopamine solution (4mg / m...

Embodiment 3

[0069] Preparation of the scaffold

[0070] The experimental procedure is the same as that in Example 1, except that the concentration of the dopamine solution immersed in the silk protein electrospinning scaffold (SF scaffold) is 2 mg / ml.

[0071] First, dissolve 8g silk protein (SF) in 80ml hexafluoroisopropanol mixed solvent to form a 10% (W / V) solution, draw the silk protein mixture into a 10ml injection syringe, and inject 1.5-2ml of it through a micro-injection pump. Propelling at a rate of / h, the inner diameter of the front needle is a stainless steel flat-head dispensing needle of 0.8mm. During the electrospinning process, the voltage was controlled at 15-20kV, and the distance from the needle to the receiver was 15-20cm. The rotating speed of the drum collector was 300-500 times per minute, and the electrospun fibers were collected on aluminum foil paper and dried in vacuum to obtain SF scaffolds.

[0072] Dissolve dopamine in Tris-buffer, and immerse 8g silk prote...

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Abstract

The invention discloses a silk fibroin-dopamine-E7 oligopeptide compound stent which comprises a silk fibroin porous stent. E7 oligopeptide is grafted to the silk fibroin porous stent through dopamine. The silk fibroin porous stent is prepared by electrospinning. The silk fibroin porous stent is impregnated in a dopamine solution and an E7 oligopeptide solution successively to obtain the stent. The compound stent not only improves the hydrophilicity and promotes cell adhesion and proliferation, but also accelerates osteogenic differentiation of BMSCs by means of joint action of dopamine and E7, and the stent has very high raising efficiency to BMSCs in vitro and in vivo, so that osteanagenesis is promoted.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a silk fibroin-dopamine-E7 short peptide composite scaffold and a preparation method and application thereof. Background technique [0002] The microenvironment in which cells live in the body is mostly a nano-scaffold structure composed of collagen fibers and other cell surfaces. In addition to protein, which is an important factor in regulating cell life activities, the nano-scale scaffold structure interface is another important factor. [0003] Nanofibers prepared by electrospinning are conducive to cell implantation, attachment, infiltration of nutrients and discharge of metabolic waste, and provide a good microenvironment for cell growth and proliferation, thereby enhancing cell adhesion, migration, and proliferation. and differentiation function. [0004] Silk protein is a natural polymer material with no toxic and side effects, but the osteoinductive properties of silk ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/34A61L27/50A61L27/56
CPCA61L27/227A61L27/34A61L27/50A61L27/56A61L2400/18A61L2430/02C08L89/00
Inventor 蒋欣泉曹玲燕吴建楠郑奥焦德龙
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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