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Method for extracting dihydromyricetin from ampelopsis grossedentata

A technology of dihydromyricetin and octopus, which is applied in the direction of organic chemistry, can solve the problems of dihydromyricetin purity and low yield, and achieve the effects of low cost, increased yield, and simple purification process

Pending Publication Date: 2019-02-22
CHANGSHA SHENGKAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the productive rate of existing dihydromyricetin extraction technology is not high, the purity of the dihydromyricetin of gained also needs to improve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting dihydromyricetin from Snake dentata grape, comprising the following steps:

[0021] (1) Pre-treatment: Bake the fresh leaves of Snakejascens at 90°C for 3 hours, then take them out and pulverize them into 20-mesh granules to prepare Snakesiassus leaves powder.

[0022] (2) Enzymatic hydrolysis: Mix the leaf powder of Grape dentata with deionized water at 40°C, add a compound enzyme accounting for 2% of the weight of the leaf powder, stir evenly, and let it stand for 1 hour to obtain an enzymatic hydrolysis solution.

[0023] (3) Extraction: filter the enzymatic hydrolysis solution with a centrifuge to obtain an aqueous solution, put the filtered solid into a hot ethanol solution with a concentration of 90%, treat it with an ultrasonic extraction tank for 1 hour, and then obtain the ethanol solution by pressure filtration. Mix with ethanol solution to obtain stock solution.

[0024] (4) Concentration: Concentrate the stock solution to 0.5 times t...

Embodiment 2

[0030] A method for extracting dihydromyricetin from Snake dentata grape, comprising the following steps:

[0031] (1) Pre-treatment: Bake the fresh leaves of Snakesia japonica at 95°C for 3 hours, then take them out and pulverize them into 20-mesh granules to obtain the powder of Snakesia sedumensis leaves.

[0032] (2) Enzymatic hydrolysis: Mix the leaf powder of Snakedon japonica with 45°C deionized water, add 2.5% compound enzyme by weight of the leaf powder, stir evenly, and let it stand for 1.5 hours to obtain an enzymatic hydrolysis solution.

[0033] (3) Extraction: filter the enzymatic hydrolysis solution with a centrifuge to obtain an aqueous solution, put the filtered solid into a hot ethanol solution with a concentration of 90%, treat it with an ultrasonic extraction tank for 1 hour, and then obtain the ethanol solution by pressure filtration. Mix with ethanol solution to obtain stock solution.

[0034] (4) Concentration: Concentrate the stock solution to 0.5 time...

Embodiment 3

[0040] A method for extracting dihydromyricetin from Snake dentata grape, comprising the following steps:

[0041] (1) Pre-treatment: Bake the fresh leaves of S. spp. vine at 100°C for 4 hours, then take them out and grind them into 20-mesh granules to obtain S. spp. leaves powder.

[0042] (2) Enzymatic hydrolysis: Mix the leaf powder of Snakeja japonica with deionized water at 50°C, add a compound enzyme accounting for 3% of the weight of the leaf powder, stir evenly, and let it stand for 2 hours to obtain an enzymatic hydrolysis solution.

[0043] (3) Extraction: filter the enzymatic hydrolysis solution with a centrifuge to obtain an aqueous solution, put the filtered solid into a hot ethanol solution with a concentration of 90%, treat it with an ultrasonic extraction tank for 1 hour, and then obtain the ethanol solution by pressure filtration. Mix with ethanol solution to obtain stock solution.

[0044] (4) Concentration: Concentrate the stock solution to 0.5 times the we...

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PUM

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Abstract

The invention discloses a method for extracting dihydromyricetin from ampelopsis grossedentata. The method comprises the following steps: (1) performing pretreatment; (2) performing enzymolysis; (3) extracting; (4) concentrating; (5) primary crystallization; (6) recrystallization; (7) purification; and (8) vacuum drying. According to the method disclosed by the invention, the yield and purity of the dihydromyricetin can be improved.

Description

technical field [0001] The invention relates to a method for extracting dihydromyricetin, in particular to a method for extracting dihydromyricetin from Grape dentata. Background technique [0002] Snake-toothed snake grape, also known as vine tea and Maoyan berry tea, is a wild woody vine, the main active ingredient of which is dihydromyricetin. Dihydromyricetin has a variety of unique effects such as scavenging free radicals, anti-oxidation, antithrombotic, anti-tumor, anti-inflammatory; and dihydromyricetin is a special kind of flavonoid compound, which is effective in relieving alcohol poisoning, preventing alcoholic liver, fat Liver, inhibiting the deterioration of liver cells, reducing the incidence of liver cancer, antihypertensive, inhibiting platelet aggregation in vitro and thrombus formation in vivo, lowering blood lipid and blood sugar levels, increasing superoxide dismutase activity, and protecting liver and other aspects have special effects . However, the yi...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/40
CPCC07D311/32C07D311/40
Inventor 尹婷
Owner CHANGSHA SHENGKAI BIOLOGICAL TECH
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