Method for extracting dihydromyricetin from ampelopsis grossedentata

A technology of dihydromyricetin and octopus, which is applied in the direction of organic chemistry, can solve the problems of dihydromyricetin purity and low yield, and achieve the effects of low cost, increased yield, and simple purification process

Pending Publication Date: 2019-02-22
CHANGSHA SHENGKAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the productive rate of existing dihydromyricetin extraction technology is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] A method for extracting dihydromyricetin from Snake dentata grape, including the following steps:

[0021] (1) Pretreatment: The fresh Snake Snake grape leaves are roasted at 90°C for 3 hours, and then taken out and crushed into 20 mesh particles to prepare Snake Snake grape leaf powder.

[0022] (2) Enzymatic hydrolysis: Mix Snake Snake grape leaf powder with deionized water at 40°C, add 2% of the weight of the leaf powder, and stir evenly, leave it for 1 hour to prepare an enzymatic hydrolysis solution.

[0023] (3) Extraction: filter the enzymatic hydrolysate with a centrifuge to obtain an aqueous solution, put the filtered solid into a hot ethanol solution with a concentration of 90%, treat it with an ultrasonic extraction tank for 1 hour, and then press filter to obtain the ethanol solution. Mix with ethanol solution to prepare the original solution.

[0024] (4) Concentration: Concentrate the stock solution to 0.5 times the weight of the raw material to ...

Example Embodiment

[0029] Example 2

[0030] A method for extracting dihydromyricetin from Snake dentata grape, including the following steps:

[0031] (1) Pretreatment: The fresh Snake Snake grape leaves are roasted at 95°C for 3 hours, and then taken out and crushed into 20 mesh particles to prepare Snake Snake grape leaf powder.

[0032] (2) Enzymatic hydrolysis: Mix Snake Snake grape leaf powder with deionized water at 45°C, add 2.5% of the weight of the leaf powder, and stir evenly, leave it for 1.5 hours to prepare an enzymatic hydrolysis solution.

[0033] (3) Extraction: filter the enzymatic hydrolysate with a centrifuge to obtain an aqueous solution, put the filtered solid into a hot ethanol solution with a concentration of 90%, treat it with an ultrasonic extraction tank for 1 hour, and then press filter to obtain the ethanol solution. Mix with ethanol solution to prepare the original solution.

[0034] (4) Concentration: Concentrate the stock solution to 0.5 times the weight of the raw materia...

Example Embodiment

[0039] Example 3

[0040] A method for extracting dihydromyricetin from Snake dentata grape, including the following steps:

[0041] (1) Pretreatment: The fresh Snake Snake grape leaves are roasted at 100°C for 4 hours, and then taken out and crushed into 20 mesh particles to obtain Snake Snake grape leaf powder.

[0042] (2) Enzymatic hydrolysis: Mix Snake Snake grape leaf powder with deionized water at 50°C, add 3% of the weight of the leaf powder, and stir evenly, leave it for 2 hours to prepare an enzymatic hydrolysis solution.

[0043] (3) Extraction: filter the enzymatic hydrolysate with a centrifuge to obtain an aqueous solution, put the filtered solid into a hot ethanol solution with a concentration of 90%, treat it with an ultrasonic extraction tank for 1 hour, and then press filter to obtain the ethanol solution. Mix with ethanol solution to prepare the original solution.

[0044] (4) Concentration: Concentrate the stock solution to 0.5 times the weight of the raw material to...

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PUM

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Abstract

The invention discloses a method for extracting dihydromyricetin from ampelopsis grossedentata. The method comprises the following steps: (1) performing pretreatment; (2) performing enzymolysis; (3) extracting; (4) concentrating; (5) primary crystallization; (6) recrystallization; (7) purification; and (8) vacuum drying. According to the method disclosed by the invention, the yield and purity of the dihydromyricetin can be improved.

Description

technical field [0001] The invention relates to a method for extracting dihydromyricetin, in particular to a method for extracting dihydromyricetin from Grape dentata. Background technique [0002] Snake-toothed snake grape, also known as vine tea and Maoyan berry tea, is a wild woody vine, the main active ingredient of which is dihydromyricetin. Dihydromyricetin has a variety of unique effects such as scavenging free radicals, anti-oxidation, antithrombotic, anti-tumor, anti-inflammatory; and dihydromyricetin is a special kind of flavonoid compound, which is effective in relieving alcohol poisoning, preventing alcoholic liver, fat Liver, inhibiting the deterioration of liver cells, reducing the incidence of liver cancer, antihypertensive, inhibiting platelet aggregation in vitro and thrombus formation in vivo, lowering blood lipid and blood sugar levels, increasing superoxide dismutase activity, and protecting liver and other aspects have special effects . However, the yi...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/40
CPCC07D311/32C07D311/40
Inventor 尹婷
Owner CHANGSHA SHENGKAI BIOLOGICAL TECH
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