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Method for grafting DNA on polydopamine nanoparticle surface with controllable density

A nanoparticle and polydopamine technology, applied in the field of nanobiology, can solve the problems of research, controllable density of DNA grafting on the surface of PDA nanoparticles, etc., and achieve the effect of broad application space

Active Publication Date: 2019-02-22
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This study proved that the surface DNAization of nanoparticles by PDA coating is completely feasible, but it did not study the controllable DNA grafting density on the surface of PDA nanoparticles. Research on key issues in combination is still blank

Method used

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  • Method for grafting DNA on polydopamine nanoparticle surface with controllable density
  • Method for grafting DNA on polydopamine nanoparticle surface with controllable density
  • Method for grafting DNA on polydopamine nanoparticle surface with controllable density

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-6

[0079] A DNA-grafted polydopamine nanoparticle, the preparation method of which is as follows:

[0080] (1) Preparation of polydopamine nanoparticles with a particle size of 250nm

[0081] Add 500.0mg dopamine hydrochloride to the mixed solution of 100.0mL ultrapure water, 40.0mL ethanol and 1.0mL ammonia water, centrifuge (48000RCF) and wash three times with ultrapure water after sealing at room temperature and stirring for 24 hours, wherein, the polydopamine nanoparticle surface of gained Zeta potential was -15.43±1.61mV.

[0082] (2) Preparation of ssDNA1 grafted polydopamine nanoparticles

[0083] The concentration of polydopamine nanoparticles is controlled to be 50.0 μg / mL, the concentration of DNA is 1.0 μM, the reaction is carried out in Tris-HCl (10.0 mM) buffer solution with pH=8.5, sodium chloride is added, and the total volume of the reaction system is controlled to be 100 μL , and the reaction time was 8 hours; after that, it was centrifuged (48000RCF) and washe...

Embodiment 7-12

[0086] The only difference from Examples 1-6 is that in this example, ssDNA1 is replaced by ssDNA2.

Embodiment 13-18

[0088] The only difference with Example 1 is that in this example, sodium chloride is replaced by magnesium chloride, and the concentration of magnesium chloride is controlled to be 0.001mol / L (embodiment 13), 0.005mol / L (embodiment 14), 0.025mol / L (Example 15), 0.1mol / L (Example 16), 0.5mol / L (Example 17), 2mol / L (Example 18).

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Abstract

The invention provides a method for grafting DNA on polydopamine nanoparticle surface with controllable density. According to the method, the grafting density of DNA on polydopamine nanoparticle surface is controlled in the manner of adding metal cations into a reaction system. According to the method provided by the invention, the DNA grafted polydopamine nanoparticle with controllable grafting density can be acquired, wherein the maximal grafting density of DNA on the surface of polydopamine nanoparticle in grain size of 250nm can reach up to 34.60pmol / cm2 and is far more than the maximal grafting density (21pmol / cm2) of DNA on the surface of gold nanoparticle in grain size of 250nm.

Description

technical field [0001] The invention belongs to the field of nano-biotechnology, and relates to a method for grafting DNA on the surface of polydopamine nano particles with controllable density. Background technique [0002] Deoxyribonucleic acid (DNA) is the most important biological macromolecule. From the perspective of materials science, DNA molecules have the advantages of programmable design, rich biological functions, and good biocompatibility. Many nanomaterials such as gold nanoparticles, iron oxide nanoparticles, and quantum dots often have special electrical, optical, thermal, and magnetic properties. Combining DNA on the surface of nanoparticles to form DNA nanoparticles can greatly expand the application space of DNA; at the same time, DNA can also greatly improve the water dispersibility and biocompatibility of these exogenous particles, making them more biologically application potential. At present, DNA nanoparticles have been widely used in the fields of n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/06
CPCC08G73/0672
Inventor 田雷蕾邵晨邹尚瀚锋
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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