A method for rapid application of solid culture products of Animidilia eziri to prevent and control pine wilt disease

A nematicide and solid culture technology, applied in the field of microbial biological control, can solve the problems of reducing manpower, material resources, and the inability to directly apply biological control to solid cultured products, and achieve the advantages of reducing manpower, improving work efficiency, and extending storage period. Effect

Active Publication Date: 2022-02-08
成昌根
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

During the application, only drilling and nailing are required, and there is no need to use a high-pressure plug, which greatly reduces manpower and material resources; at the same time, the storage period of the product is greatly extended, and the biological activity of the preparation is significantly improved; it solves the problem that the solid culture product cannot be directly Difficulties applied to biological control

Method used

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  • A method for rapid application of solid culture products of Animidilia eziri to prevent and control pine wilt disease
  • A method for rapid application of solid culture products of Animidilia eziri to prevent and control pine wilt disease
  • A method for rapid application of solid culture products of Animidilia eziri to prevent and control pine wilt disease

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Embodiment 1

[0034] A method for rapidly applying the solid culture product of Anidida iridii to prevent and treat pine wilt disease, comprising the pre-obtained Amynidida iridii bacterium colony, comprising the following steps:

[0035] (1) Pretreatment: Sterilize the operation room, ultra-clean workbench, solid stirring fermenter and all equipment used in subsequent steps, put all sterilized equipment into the ultra-clean workbench, and turn on the ultraviolet lamp , blast; operators wear sterilized overalls, caps, masks and gloves;

[0036] (2) Strain preparation: Weigh 27g of PDB powder, dissolve it in 1L of distilled water, and distribute it in a culture container, and sterilize it under high temperature and high pressure for 20 minutes; after cooling, insert the colonies of Animidilia eideri, and place it on a shaker for cultivation For 6 days, the culture temperature was 26°C, and the shaker speed was 120 rpm; after the culture was completed, the strains of Animidobacter isobacillus...

Embodiment 2

[0049] A method for rapidly applying the solid culture product of Anidida iridii to prevent and treat pine wilt disease, comprising the pre-obtained Amynidida iridii bacterium colony, comprising the following steps:

[0050] (1) Pretreatment: Sterilize the operation room, ultra-clean workbench, solid stirring fermenter and all equipment used in subsequent steps, put all sterilized equipment into the ultra-clean workbench, and turn on the ultraviolet lamp , blast; operators wear sterilized overalls, caps, masks and gloves;

[0051] (2) Strain preparation: Weigh 27g of PDB powder, dissolve it in 1L of distilled water, and distribute it in a culture container, and sterilize it under high temperature and high pressure for 20 minutes; after cooling, insert the colonies of Animidilia eideri, and place it on a shaker for cultivation For 5 days, the culture temperature was 26°C, and the shaker speed was 120 rpm; after the culture was completed, the strains of Animidobacter isobacillus...

Embodiment 3

[0064]A method for rapidly applying the solid culture product of Anidida iridii to prevent and treat pine wilt disease, comprising the pre-obtained Amynidida iridii bacterium colony, comprising the following steps:

[0065] (1) Pretreatment: Sterilize the operation room, ultra-clean workbench, solid stirring fermenter and all equipment used in subsequent steps, put all sterilized equipment into the ultra-clean workbench, and turn on the ultraviolet lamp , blast; operators wear sterilized overalls, caps, masks and gloves;

[0066] (2) Strain preparation: Weigh 27g of PDB powder, dissolve it in 1L of distilled water, and distribute it in a culture container, and sterilize it under high temperature and high pressure for 20 minutes; after cooling, insert the colonies of Animidilia eideri, and place it on a shaker for cultivation For 6 days, the culture temperature was 26°C, and the shaker speed was 120 rpm; after the culture was completed, the strains of Animidobacter isobacillus ...

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Abstract

The invention discloses a method for rapidly applying a solid culture product of Animidilia eziri to prevent and treat pine wilt disease, and belongs to the technical field of microbial biological control. In the present invention, after adding a certain proportion of nutrients, protective agents, growth stimulating factors, sporulation stimulating factors, and adhesives to the solid culture product and mixing them uniformly, adding a harmless plasticizer to the solid culture product, and mixing it Pressing and processing into a long rod-shaped product, and finally wrapping a layer of glutinous rice paper on its surface, the entire production process is isolated from the external environment, without manual operation, reducing pollution caused by human factors or exposure to the external environment. During the application, only drilling and nailing are required, without the use of high-pressure plugs, which greatly reduces manpower and material resources; at the same time, the storage period of the product is greatly extended, and its biological activity is significantly improved; it solves the problem that the solid culture product cannot be used directly The problem of biological control. When the present invention is applied to inter-forest prevention and control work, the operation is simple and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of microbial biological control, and in particular relates to a method for preventing and treating pine wilt disease by using a solid fermentation product of the pine wood nematode parasitic fungus-Animidilia eziri. Background technique [0002] Pine wilt disease, also known as pine wood nematode disease, is a dangerous disease caused by pine wood nematode (Bursaphelenchus xylophilus) that causes rapid death and continuous destruction of pine trees. It is mainly transmitted by insects such as monochamus. The disease was first reported in Japan in 1905, and quickly spread to China, South Korea, Vietnam, Europe and other countries and regions, seriously threatening the safety of pine forests worldwide. The disease has the characteristics of multiple transmission routes, rapid onset, and difficult treatment. Up to now, there is still no effective prevention and treatment method. It is called pine cancer. my co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P5/00A01G7/06A01G13/00C12R1/01
CPCA01G7/06A01G13/00A01N63/00C12N1/20
Inventor 王海华殷灿王云波陶然高杰段贝贝王春燕王振成昌根
Owner 成昌根
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