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Drug-resistant bacteria active substance penicipyrroether A as well as preparation and application thereof

A technology of penicillin ether and active substances, which is applied in the application field of preparing anti-methicillin-resistant Staphylococcus aureus drugs, and can solve the problems of enhanced drug resistance of MRSA

Active Publication Date: 2019-02-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vancomycin has always been the gold standard drug for the treatment of methicillin-resistant Staphylococcus aureus infection. However, due to the frequent use of vancomycin in clinical practice, the resistance of MRSA to it is constantly increasing.

Method used

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  • Drug-resistant bacteria active substance penicipyrroether A as well as preparation and application thereof
  • Drug-resistant bacteria active substance penicipyrroether A as well as preparation and application thereof
  • Drug-resistant bacteria active substance penicipyrroether A as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1. Isolation and cultivation of Penicillium griseofulvum ZZ380

[0036]Get weighed thick-legged crab (Pachygrapsus crassipes) (20.1 grams), soak in 75% ethanol for 10 seconds to remove surface microorganisms, then wash three times with sterile water and then homogenate, take the upper layer liquid after centrifugation to prepare volume The concentration is 1×10 -1 , 1×10 -2 , 1×10 -3 sample solution (take 1mL homogenate and centrifuge the upper layer liquid, add 9mL sterile water to make a volume concentration of 10 -1 sample solution, and diluted step by step to obtain a volume concentration of 10 -2 and 10 -3 sample solution). Take 200 μL of sample solutions of various concentrations and evenly disperse them in a petri dish containing solid medium of potato dextrose agar (PDA, 6 g of potato flour, 20 g of glucose, 20 g of agar, 0.1 g of chloramphenicol, purchased from Hangzhou Microbial Reagent Co., Ltd.). After culturing at 28°C for 5 days, different...

Embodiment 2

[0037] Example 2. Identification of bacterial species of Penicillium griseofulvum ZZ380

[0038] The species of the obtained strain ZZ380 was identified by ITS rDNA sequence analysis method.

[0039] 2.1 Experimental reagents and instruments

[0040] PCR reagent: PrimeSTAR Max DNAPolymerase (TaKaRa), primer (Invitrogen synthesis), primer sequence is:

[0041]

[0042] Marker: DL2000

[0043] Experimental equipment: centrifuge, electrophoresis instrument, PCR instrument, ABI 3730XL sequencer.

[0044] 2.2 Experimental steps

[0045] 2.2.1 Extraction of fungal genomic DNA

[0046] Using the Ezup Column Fungal Genomic DNA Extraction Kit (Shenggong), the fungi were ground with liquid nitrogen before use.

[0047] 2.2.2 Concentration and quality detection of fungal genomic DNA

[0048] DNA concentration and quality detection was performed using a Nanodrop ultra-micro spectrophotometer.

[0049] 2.2.3 PCR amplification

[0050] a. PCR reaction system

[0051]

[0052]...

Embodiment 3

[0066] Embodiment 3. Preparation of Penicillium griseofulvum ZZ380 fermentation broth

[0067] Pick Penicillium griseofulvum ZZ380 on the PDA solid slant medium and inoculate it into a 500mL Erlenmeyer flask containing 250mL potato-dextrose broth (PDB, 100g potato, 10g glucose, 35g sea salt, 1L water) liquid medium middle. The culture solution containing the ZZ380 strain was cultivated with rotation (180 rpm) and shaking at 28° C. for 3 days to obtain a strain liquid. Transfer 5 mL of the seed liquid into a 500 mL Erlenmeyer flask containing 250 mL of PDB, and culture it statically at 28°C for 30 days to obtain a fermentation liquid containing the active substance penicillin ether A.

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PUM

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Abstract

The invention provides drug-resistant bacteria active substance penicipyrroether A which is a marine natural active substance with a novel structure and is obtained by a specific isolation culture method by utilizing a known marine penicillium griseofulvum strain ZZ380; the compound is proved for the first time to have a strong inhibitory activity on the growth of methicillin-resistant Staphylococcus aureus, and the effect is particularly remarkable. The drug-resistant bacteria active substance penicipyrroether A provided by the invention overcomes the defect of drug resistance of an existingantibiotic drug to methicillin-resistant Staphylococcus aureus infection, has a wider application prospect in the preparation of drug-resistant bacteria and can provide a novel drug molecule for effective treatment of the methicillin-resistant Staphylococcus aureus infection. A chemical structural formula of the penicipyrroether A is as shown in the description.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the marine active compound penicillin ether A (Penicipyrroether A) for resisting drug-resistant bacteria, the preparation of the compound and the application in the preparation of drugs for resisting methicillin-resistant Staphylococcus aureus. Background technique [0002] The resistance of pathogenic bacteria to antibiotics caused by the abuse of antibiotics is increasing, and the types and numbers of drug-resistant mutant strains are increasing, which has become a major problem that threatens human health. Methicillin-resistant Staphylococcus aureus (MRSA), known as a superbug, accounts for up to 60% of cases of Staphylococcus aureus infection, and 30% of the cases are resistant to vancomycin. Drug resistance has emerged and is on the rise year by year. Vancomycin has always been the gold standard drug for the treatment of methicillin-resistant Staphylococcus aureus infection. However, ...

Claims

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Application Information

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IPC IPC(8): C07J71/00C12P33/20A61P31/04C12R1/80
CPCA61P31/04C07B2200/07C07J71/0057C12P33/20
Inventor 张治针宋腾飞陈梦宣连晓媛
Owner ZHEJIANG UNIV
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