Nano-liposome containing cell penetrating peptide and preparation method and application thereof

Abstract

The invention discloses a nano-liposome containing cell penetrating peptide. The nano-liposome is prepared from lecithin, a surface active agent, vitamin E and the cell penetrating peptide in the weight ratio of (10-60) to (10-30) to (0.1-10) to (0.2-20); and the cell penetrating peptide is selected from at least one of biological source cell penetrating peptide, chimeric cell penetrating peptideand artificially synthesized cell penetrating peptide. According to a preparation method of the nano-liposome, a brand-new high pressure homogenization dispersion method is utilized, the stability ofthe nano-liposome is greatly improved, and the nano-liposome can be applied to a tank-mix assistant or a fly prevention assistant agriculturally. The nano-liposome containing the cell penetrating peptide has the high stability, can be stored for a long time at room temperature, is not sensitive to changes of pH value and ionic strength, and has good compatibility with pesticides and / or fertilizers. No organic solvents are needed in the preparation process, and no pollution is generated. The nano-liposome can be transmitted fast in vivo after being applied to crops, and reaches the minimum effective drug concentration rapidly, prolongs the persistence time, improves the prevention and treatment effect and delays occurrence of drug resistance.

Description

technical field [0001] The invention belongs to the field of agriculture, and in particular relates to a nanoliposome containing a cell-penetrating peptide, a preparation method thereof, and an application of the nanoliposome containing a cell-penetrating peptide. Background technique [0002] Nanoliposome is a phospholipid bilayer structure artificially produced by simulating cell membrane structure. As a kind of drug carrier with excellent biocompatibility, nanoliposome has been widely used in the medical field. Nano-liposomes can carry and embed hydrophilic, hydrophobic and amphiphilic molecules, especially for molecules with poor water solubility and oil-water insoluble molecules, which can assist them to quickly penetrate the surface of organisms and quickly in vivo distributed. Nano-liposomes can make the drug quickly reach the minimum effective drug concentration, effectively prolong the maintenance time of the effective drug concentration, enhance the effect of dru...

AI Technical Summary

Problems solved by technology

However, cell-penetrating peptides cannot penetrate the waxy layer or stratum corneum on the surface of organisms, so they can be covalently bonded or mixed with active ingredients of pesticides and / or fertilizers alone, which cannot help them enter the organism and play a role in assisting exogenous function of entry into cells

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