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A kind of preparation method of double fluorescence labeled nano material

A nano-material and dual-fluorescence technology, applied in the field of fluorescent materials, can solve problems such as narrow excitation spectrum, overlapping peaks, and limited application range, and achieve high sensitivity and good slow-release results

Active Publication Date: 2021-03-16
江西中洪博元生物技术有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Traditional organic fluorescent labeling materials limit their application scope due to some of their own properties, such as: narrow excitation spectrum, broad emission spectrum, overlapping peaks caused by tailing, easy photobleaching and photolysis, etc.

Method used

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  • A kind of preparation method of double fluorescence labeled nano material
  • A kind of preparation method of double fluorescence labeled nano material
  • A kind of preparation method of double fluorescence labeled nano material

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Embodiment Construction

[0033] Specific embodiments of the present invention will be described in detail below.

[0034] A method for preparing a double-fluorescence-labeled nanomaterial, comprising the steps of:

[0035] (1) Dissolve HP-β-CD in DMSO first, then add triethylamine, dissolve and mix completely, and set aside; dissolve CDI in DMSO, and add it to the solution in the previous step after it is completely dissolved; then mix the liquid Stir in the dark under the protection of nitrogen, add an equal volume of pre-cooled ether to the mixed liquid, mix well to produce a precipitate, then filter, and then dissolve the filtered HP-β-CD-CDI sample in DMSO to form HP-β- DMSO solution of CD-CDI;

[0036] (2) Dissolve PEI in DMSO first, then add triethylamine, completely dissolve and mix well, then add drop by drop to HP-β-CD-CDI solution under nitrogen protection and avoid light, and instill slowly for a period of time Over 2h, then stirred overnight to obtain HP-β-CD-PEI solution

[0037] (3) T...

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Abstract

The invention discloses a preparation method of a double-fluorescence-labeled nano material. The preparation method comprises the following steps: connecting an intermediate substance CDI with HP-beta-CD; enabling HP-beta-CD-CDI and PEI to react in an organic solvent to prepare an HP-beta-CD-PEI solution; after dialysis, performing freeze-drying to obtain HP-beta-CD-PEI; under the catalysis of EDC(ethylene dichloride), coupling with rhodamine B to obtain an HP-beta-CD-PEI-RhB solution; after dialysis, performing freeze-drying to obtain HP-beta-CD-PEI-RhB; adsorbing fluorescein sodium throughthe HP-beta-CD-PEI-RhB; performing dialysis and freeze-drying to obtain the double-fluorescence-labeled nano material. The double-fluorescence-labeled nano material prepared by the method has the characteristics of non-radioactive performance, high stability, high sensitivity, high selectivity and the like; when being applied to researches of medicine activity, the double-fluorescence-labeled nanomaterial has no influence on the activity of medicines.

Description

technical field [0001] The invention relates to the field of fluorescent materials, in particular to a preparation method of double fluorescent-labeled nanomaterials. Background technique [0002] Fluorescence is a common luminescent phenomenon in nature, that is, a phenomenon in which fluorescent compounds emit long-wavelength fluorescence after being irradiated by short-wavelength ultraviolet light. More precisely, fluorescence refers to some fairly bright colors of light that the human eye can see under external light, such as green, orange, and yellow. People often call them neon lights. A fluorescent material refers to a material that absorbs light of a certain wavelength and immediately emits light of a different wavelength, called a fluorescent material. [0003] Fluorescence labeling technology refers to the use of some substances that can emit fluorescence to covalently bind or physically adsorb on a certain group of the molecule to be studied, and use its fluoresc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00C09K11/06B82Y20/00B82Y30/00B82Y40/00
CPCB82Y20/00B82Y30/00B82Y40/00C08G81/00C09K11/06C09K2211/14
Inventor 江斌刘贤武
Owner 江西中洪博元生物技术有限公司
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