Application of a targeted molecular probe in vascular calcification detection products
A technology of vascular calcification and molecular probes, applied in the field of nuclear medicine, can solve the problems of poor imaging, underestimation of calcification, and inability to clearly image the boundary of the lumen, and achieve fast imaging speed, slow excretion, and avoid difficulties in identifying vascular calcification
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Embodiment 1
[0020] Example 1: 18 Synthesis of F-SFB-CML
[0021] Xiang Shengyou 18 SFB (10 mg SFB, dissolved in 1 mL acetonitrile) was added to the container of F ion, and reacted at 90°C for 7 min. After the reaction was completed, it was cooled to room temperature. Then add 6mL 0.1M HCl, ventilate and stir for 1 min, and pass the reaction solution through a C18 column (washed with a mixture of 7.5mL 0.1M HCl and 2.5mL acetonitrile before use) to the waste bottle. Then add 3 mL of acetonitrile to flow through the C18 column, and elute the intermediate product to the No. 2 reaction tube (pre-add 40 μL of tetrapropylammonium hydroxide TPAOH).
[0022] Heat and dry to remove acetonitrile, then add TSTU solution (dissolve 10 mg TSTU, ie 2-succinimidyl-1,1,3,3-tetramethylurea tetrafluoroborate in 1mL acetonitrile), and react at 90℃ After 7 minutes, the reaction was cooled to room temperature. Add 5mL 0.1M HCl, ventilate and stir for 1 min, then pass the reaction solution through a C18 column (wa...
Embodiment 2
[0033] Example 2: ApoE - / - Mouse micro PET scan
[0034] (1) Construction of an animal model of vascular calcification
[0035] The male apoE- / - mice used in this experiment were all from the Experimental Animal Center of Jiangsu University. Feeding conditions: temperature 22±2℃, humidity 40-60%, 12-hour cycle lighting, ordinary diet, free intake of food and water. All items entering the SPF room must be sterilized by high temperature and high pressure, and strict aseptic operations are implemented. At 6 weeks of age, mice in the experimental group were given intraperitoneal injection of streptozotocin (STZ, dissolved in 0.05 mol / L pH 4.5 citrate buffer) 40 mg / kg / day for 5 consecutive days. Blood glucose level after 2 weeks> Mice with 300 mg / dL were included in this study and were fed with regular diet for 24 months.
[0036] (2) 18 F-SFB-CML micro PET imaging
[0037] Weigh before scanning, isoflurane / oxygen mixed gas anesthesia (1.5-2.0%), tail vein injection 18 F-SFB-CML (inject...
Embodiment 3
[0045] Example 3: LDLR + / - Hamster micro PET / CT scan
[0046] The male LDLR+ / - hamsters used in this experiment were all from the Experimental Animal Center of Jiangsu University. At 6 weeks of age, the hamsters in the vascular calcification group were given intraperitoneal injection of streptozotocin (STZ, dissolved in 0.05 mol / L pH 4.5 citrate buffer) 40 mg / kg / day for 5 consecutive days. Blood glucose level after 2 weeks> Mice with 300 mg / dL were included in this study, and the control group was 6-week-old male LDLR + / - hamsters fed with regular diet for 48 months.
[0047] After modeling, the hamsters were anesthetized with isoflurane. After the righting reflex disappeared, the contrast agent (about 200μci each) was injected into the sublingual vein and fixed to the micro PET / CT scanning bed. The acquisition method was 2h static multi-dimensional scanning.
[0048] Compared 18 F-SFB-CML and 18 According to the results of F-NaF microPET / CT scan, the hamsters in the control group c...
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