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Method for rapidly excavating and measuring adenosylmethionine in Baijiu making process

An adenosylmethionine and liquor technology, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex technical operation, high equipment requirements, large workload, etc. Accurate effect

Active Publication Date: 2019-03-08
BEIJING SHUNXIN AGRICULTURE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, various domestic liquor companies or scientific research institutes of universities have continuously explored and discovered the flavor substances in liquor, hoping to discover new functional factors. Currently, the new flavor substances discovered in the liquor brewing process have complex technical operations and heavy workload. , high equipment requirements and high cost

Method used

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  • Method for rapidly excavating and measuring adenosylmethionine in Baijiu making process
  • Method for rapidly excavating and measuring adenosylmethionine in Baijiu making process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Determination method of adenosylmethionine content in fermented grains in the production of Niulanshan Baijiu.

[0034] Weigh 10g of wine fermented grains containing adenosylmethionine after genomic and transcriptome analysis into a 50mL Erlenmeyer flask, add 20ml of ultrapure water, soak for 30min, ultrasonically extract 3 times, each time for 20min, centrifuge at 12000rpm for 15min and take The supernatant is ready for use; add 2ml of methanol to the oasis HLB extraction column to activate the solid phase extraction column, and then add 1ml of ultrapure water to balance the extraction column; add the supernatant obtained by the above centrifugation, let stand for 10min, add 2ml of methanol to elute, and then add 1ml of mobile phase (water (containing 0.1% formic acid):methanol=(95:5, v / v)) to elute, and collect 0.8ml of effluent; pass through a 0.22μm filter membrane and put it in a liquid phase vial for LC-MS detection Standby; follow the LC- MS analysis conditions ...

Embodiment 2

[0036] Determination method of adenosylmethionine content in distiller's grains in the production of Niulanshan liquor.

[0037] Weigh 8g of distiller's grains containing adenosylmethionine after genomic and transcriptome analysis into a 50mL Erlenmeyer flask, add 18ml of ultrapure water, soak for 20min, ultrasonically extract twice, each time for 25min, centrifuge at 12,000rpm for 13min and take out The supernatant is ready for use; add 2ml of methanol to the oasis HLB extraction column to activate the solid phase extraction column, and then add 1ml of ultrapure water to balance the extraction column; add the supernatant obtained by the above centrifugation, let it stand for 10min, add 2ml of methanol to elute, and then add 1ml Elute with mobile phase (water (containing 0.1% formic acid):methanol=(95:5, v / v)), and collect 0.8ml of the effluent; pass through a 0.22μm filter membrane and put it in a liquid phase vial for LC-MS detection for later use ; According to (column temp...

Embodiment 3

[0039] Determination method of adenosylmethionine content in Daqu in the production of Niulanshan Baijiu.

[0040] Weigh 5g of Daqu containing adenosylmethionine after genome combined with transcriptome analysis into a 50mL Erlenmeyer flask, add 15ml of ultrapure water, soak for 10min, ultrasonically extract twice, each time for 20min, centrifuge at 12,000rpm for 10min and take out The supernatant is ready for use; add 2ml of methanol to the oasis HLB extraction column to activate the solid phase extraction column, and then add 1ml of ultrapure water to balance the extraction column; add the supernatant obtained by the above centrifugation, let it stand for 10min, add 2ml of methanol to elute, and then add 1ml Elute with mobile phase (water (containing 0.1% formic acid):methanol=(95:5, v / v)), and collect 0.8ml of the effluent; pass through a 0.22μm filter membrane and put it in a liquid phase vial for LC-MS detection for later use ; According to (column temperature: 25°C; mobi...

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Abstract

The invention relates to the technical field of excavation and analysis of Baijiu fermentation function factors, in particular to a method for excavating adenosylmethionine in Baijiu fermentation products such as yeast, fermented grains and distillers' grains and measuring the content of the adenosylmethionine. The method particularly includes the steps: excavating existence and expression of theadenosylmethionine in the Baijiu fermentation process; measuring the content of the adenosylmethionine in the Baijiu fermentation process. The method has the advantages that the method is simple and rapid to operate, low in labor intensity, less in organic solvent dosage, high in accuracy, accurate in quantification and the like. The method is applicable to excavation of health factors and qualitative and quantitative analysis of the adenosylmethionine in the Baijiu fermentation process.

Description

technical field [0001] The invention relates to the technical field of mining and analysis of liquor fermentation function factors, in particular to the determination of the content of adenosylmethionine used in liquor fermentation products, including Daqu, distiller's grains and distiller's grains. Background technique [0002] Chinese liquor (Chinese liquors) refers to the distilled wine made from grains and grains as the main brewing raw materials, with Daqu, Xiaoqu or bran koji and wine mother as saccharification and fermentation agents, which are cooked, saccharified, fermented and distilled. The brewing of liquor has a long history. During thousands of years of inheritance and development, Chinese liquor has formed its own unique solid-state fermentation (Solid-state fermentation) method and distillation process (Distillation), which is similar to French brandy (Brandy) and Russian vodka. (Vodka) and Scotch whiskey (Whisky) are known as the world's four major distilled...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N2030/062
Inventor 魏金旺王瑛周森胡佳音朱婷婷高畅
Owner BEIJING SHUNXIN AGRICULTURE CO LTD