Kit capable of rapidly screening insecticides for controlling nilaparvata lugens and method thereof

A technology for insecticides and brown planthoppers, applied in the field of kits for rapid screening of insecticides for the control of brown planthoppers, can solve the problems of long distances, time-consuming and laborious, and death of test insects, and achieve long shelf life, simple storage conditions, and simple operations Effect

Inactive Publication Date: 2019-03-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to bring the brown planthopper from the field back to the laboratory, the journey is long, and it is easy to cause the death of a large number of test insects, which is time-consuming and labor-intensive.

Method used

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  • Kit capable of rapidly screening insecticides for controlling nilaparvata lugens and method thereof
  • Kit capable of rapidly screening insecticides for controlling nilaparvata lugens and method thereof
  • Kit capable of rapidly screening insecticides for controlling nilaparvata lugens and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The drug content selection of the drug film in the embodiment 1 nitenpyram detection bottle:

[0054] 1) Take a certain amount of nitenpyram former drug and film-forming agent for subsequent use;

[0055] 2) Put the original insecticide in a centrifuge tube, add acetone to dilute to obtain a gradient dilution, set 5 concentration gradients: 0.25mg / L, 1mg / L, 4mg / L, 8mg / L, 16mg / L, and set

[0056] Blank control group, all add film-forming agent simultaneously;

[0057] 3) Apply the gradient concentration liquid medicine evenly on the bottle bottom and the bottle wall, form a film and dry it to obtain the gradient concentration nitenpyram glass bottle;

[0058] 4) Inhale 15-20 healthy 3rd-instar nymphs of brown planthopper (indoor sensitive strain) into glass bottles of different concentrations, check the mortality rate after 55-65 minutes of cultivation, record the number of live insects, the mortality rate of the control group is ≤5%, and calculate the detection bottle ...

Embodiment 2

[0072] Embodiment 2 Utilizes the method of embodiment 1 and the preferred amount of detection bottle preparation kit

[0073] Kits for rapid screening of insecticides for the control of brown planthopper include flukes, white cardboard, writing brush, nitenpyram test bottle, dinotefuran test bottle, thiamethoxam test bottle, clothianidin test bottle, chlorpyrifos test bottle, isopropyl Vial test bottle, cyclopyrid test bottle, sulfoxaflor test bottle, etofenprox test bottle and corresponding control bottle; the drug content of the drug film in the 9 test bottles and the weight ratio of insecticide to film-forming agent They are:

[0074] Etoproxil 171.23ng / cm 2 , the weight ratio of etofenprox and film former is 1:1841;

[0075] Thiamethoxam 983.8ng / cm 2 , Thiamethoxam and film-forming agent weight ratio are 1:195;

[0076] Clothianidin 402.13ng / cm 2 , Clothianidin and film former weight ratio are 1:1160;

[0077] Nitenpyram 13.65ng / cm 2 , nitenpyram and film former wei...

Embodiment 3

[0085] Embodiment 3 checks the shelf life of above-mentioned kit

[0086] Select any of the test bottles in the above kits and the corresponding glass tubes prepared without film-forming agents and store them in a dry and dark place indoors, and test them every other month.

[0087] The results showed that the shelf life of the drug-coated glass tube prepared without film-forming agent was 2 months, and the shelf-life of the drug-coated glass tube prepared with film-forming agent was 10 months. Such as figure 1 Shown: The shelf life of the detection bottle added to the film-forming agent preparation kit is longer than that of the reagent glass tube prepared without adding the film-forming agent.

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Abstract

The invention discloses a kit capable of rapidly screening insecticides for controlling nilaparvata lugens and a method thereof. The kit comprises nine detection bottles and nine control bottles corresponding to the detection bottles, wherein nine kinds of insecticide films are covered on the inner walls and the bottom surfaces of nine detection bottles; each insecticide film contains one insecticide; the insecticides comprise nitenpyram, dinotefuran, thiamethoxam, clothianidin, chlorpyrifos, isoprocarb, cycloxaprid, fluridinitrile or ethofenprox. The kit is easy to operate, is easily popularized to farmers, can be used in the fields, does not need to be completed in the room, is capable of reducing time and detecting the sensibility on the insecticides of nilaparvata lugens in short time,and is simple in storage condition and long in shelf life.

Description

technical field [0001] The invention relates to the field of plant protection and pesticide science, in particular to a kit for rapidly screening insecticides for controlling brown planthopper and a method thereof. Background technique [0002] Brown planthopper Nilaparvata lugens Hemiptera planthoppers. Brown planthopper is a monophagous pest, which can only feed and reproduce on rice and common wild rice, and has a serious impact on rice fields in my country and most Southeast Asian countries. For a long time, the control of BPH has been dominated by chemical control, but due to the long-term and extensive use of insecticides, the sensitivity of BPH to various insecticides has decreased year by year. The results of field monitoring of insecticide resistance of N. lugens in recent years show that it has developed moderate or even high levels of resistance to some commonly used insecticides. Therefore, rapid and accurate detection of the sensitivity of N. lugens to diffe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 万虎何顺李建洪任志杰毛凯凯
Owner HUAZHONG AGRI UNIV
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