PUFA oil suspension containing sialic acid and preparation method of PUFA oil suspension containing sialic acid
A sialic acid and oil suspension technology, applied in the field of food additives and their preparation, can solve the problems of uneven oil layering, affecting the quality of oil suspension and subsequent application, etc., and achieve the effects of being difficult to decompose, stable in texture, and not easy to be damaged by oxidation.
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Embodiment 1
[0051] This embodiment provides a PUFA oil suspension containing sialic acid and a preparation method thereof, wherein the PUFA oil is DHA oil; the specific steps are as follows:
[0052] 1) Take 20g of sialic acid powder, mix it with 160g of DHA oil, and shear it at 17000rpm until it is fully dispersed;
[0053] 2) Take the dispersed sialic acid and DHA oil mixture in step 1) and perform high-speed grinding, under nitrogen protection, at a speed of 2000r / min, with a filter screen of 0.4mm, and circularly grind for 30mins.
[0054] The sialic acid powder and DHA oil in this example are all commercially available; the sialic acid content in the sialic acid powder is >98%, and the DHA content in the DHA oil is >35%.
Embodiment 2
[0056] This embodiment provides a PUFA oil suspension containing sialic acid and a preparation method thereof, the PUFA oil is DHA oil; the difference from Example 1 is only:
[0057] The sialic acid powder adopted in the present embodiment is prepared by the following method:
[0058] Escherichia coli is used as the fermentation cell, and the preservation number is CCTCC NO: M 201810. The seed liquid is added to the fermenter containing the sterilized fermentation medium at an inoculation amount of 1%. The LB medium contains: peptone 10g / L, yeast Extract powder 5g / L, sodium chloride 10g / L, pH 7.0, fermented at 37°C, supplemented with glucose at a rate of 2.5g / (L*h) by fed-batch feeding, continuously After 75 hours of fermentation, the tank was put into a tank to obtain a fermented liquid containing sialic acid. The fermented liquid was separated by membrane filtration to separate the bacteria residue, and the filtered liquid was then sterilized, decolorized, filtered, concent...
Embodiment 3
[0062] This embodiment provides a PUFA oil suspension containing sialic acid and a preparation method thereof, the PUFA oil is DHA oil; the difference from Example 2 is only:
[0063] The DHA oil that adopts in the present embodiment is prepared by following method:
[0064] Using Schizochytrium as the fermentation cell, the seed liquid was added to the fermenter containing the steam-sterilized fermentation medium. The formula of the fermentation medium was as follows: glucose 100g / L, yeast extract 6g / L, glutamine Sodium bicarbonate 20g / L, sodium chloride 3g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 5g / L, calcium chloride 1g / L, sodium bicarbonate 0.5g / L, sodium sulfate 7g / L, ammonium sulfate 8g / L, potassium chloride 0.6g / L, pH natural; control the ratio of seed liquid to fermentation medium to 5% and the ratio of dissolved oxygen to 8% during the fermentation process, put it into the tank after fermentation at 28°C for 100 hours, centrifuge or filter, and colle...
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