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A kind of preparation method of rice protein

A rice protein and rice technology, applied in the food field, can solve problems such as poor taste, low degree of hydrolysis, loss of nutrients, etc., and achieve the effects of improving purity and quality, improving solubility and degree of hydrolysis, and reducing bitterness

Active Publication Date: 2021-03-26
湖南金代科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are still many defects in the current rice protein preparation process, such as the low degree of hydrolysis, the bitter taste of rice protein, which leads to poor taste, and the preparation process leads to nutrient loss, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Rice pretreatment: Soak the rice at 10°C for 2 hours, control the water content of the rice to 25% after soaking, pulverize, and pass through an 80-mesh sieve; mix the sieved rice powder with water according to the ratio of material to liquid at 1:4, Stand still for 5h;

[0024] 2) Enzymatic removal of impurities: Add 20 U / g rice flour amylase and 30 U / g rice flour glucoamylase to the mixed solution obtained in step 1). Control the temperature at 45°C, adjust the pH to 7.0, stir for 2 hours, raise the temperature to 80°C after the reaction, and inactivate the enzyme for 15 minutes;

[0025] 3) Washing: filter step 2) to obtain the mixed solution, and repeatedly wash the residue with water, and use plate and frame filter press to remove the washing solution;

[0026] 4) Ultrasonic compound enzymolysis: add water to the slag phase treated in step (3) to adjust the mass concentration to 60%, use 400W ultrasonic treatment for 5min, add 200U / g rice powder protease, and 1...

Embodiment 2

[0031] 1) Rice pretreatment: Soak the rice at 10°C for 2.5 hours, control the water content of the rice to 25% after soaking, crush it, and pass it through an 80-mesh sieve; mix the sieved rice powder with water according to the ratio of material to liquid 1:5 , stand still for 5h;

[0032] 2) Enzymatic removal of impurities: Add 20 U / g rice flour amylase and 30 U / g rice flour glucoamylase to the mixed solution obtained in step 1). Control the temperature at 45°C, adjust the pH to 7.0, stir for 2 hours, raise the temperature to 80°C after the reaction, and inactivate the enzyme for 15 minutes;

[0033] 3) Washing: filter step 2) to obtain the mixed solution, and repeatedly wash the residue with water, and use plate and frame filter press to remove the washing solution;

[0034] 4) Ultrasonic compound enzymolysis: add water to the slag phase treated in step (3) to adjust the mass concentration to 50%, use 400W ultrasonic treatment for 5min, add 250U / g rice powder protease, and...

Embodiment 3

[0039] 1) Rice pretreatment: Soak the rice at 10°C for 2 hours, control the water content of the rice to 20% after soaking, pulverize, and pass through an 80-mesh sieve; mix the sieved rice powder with water according to the ratio of material to liquid at 1:4, Stand still for 5h;

[0040] 2) Enzymatic removal of impurities: add 20 U / g rice flour amylase and 30 U / g glucoamylase to the mixed solution obtained in step 1). Control the temperature at 45°C, adjust the pH to 7.0, stir for 2 hours, raise the temperature to 80°C after the reaction, and inactivate the enzyme for 15 minutes;

[0041] 3) Washing: filter step 2) to obtain the mixed solution, and repeatedly wash the residue with water, and use plate and frame filter press to remove the washing solution;

[0042] 4) Ultrasonic compound enzymolysis: add water to the slag phase treated in step (3) to adjust the mass concentration to 65%, use 400W ultrasonic treatment for 5min, add 200U / g rice powder protease, and 1×10 7 CFU ...

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Abstract

The invention discloses a preparation method of rice proteins and belongs to the technical field of foods. High molecular carbohydrates in rice are removed effectively, the solubility and the degree of hydrolysis of rice proteins are improved, the bitterness of the protein hydrolysate of the rice is reduced, and the purity and quality of the protein hydrolysate of the rice are improved effectivelyby means of a treatment process of enzymatic hydrolysis and purification and ultrasonic compound enzymatic hydrolysis by improving the infiltrating process of pre-treatment. The product purity reaches over 90%, the degree of hydrolysis of product proteins reaches over 80%, the solubility reaches over 96%, and the average molecular weight ranges between 780 Da and 850 Da.

Description

technical field [0001] The invention relates to a method for preparing rice protein, which belongs to the technical field of food. Background technique [0002] my country is a big producer of rice grains and has very rich rice protein resources. Through the research and technology development of rice protein, it can be beneficial to the deep processing and comprehensive utilization of rice, thereby improving the market economic benefits of rice, and at the same time It can also be developed for my country's food industry and provide more protein production base materials and protein supplements for the nutrition and health care of our people. Therefore, the deep processing of rice protein has a very broad market development prospect. [0003] The biological value (B.V.) and protein value (P.V.) of rice protein rank first among all kinds of grain proteins. The protein in rice contains all the amino acids needed by the human body. Can cause unique properties such as flatulenc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C12R1/125
CPCC07K1/145C12P21/06
Inventor 谢艳萍张国军谭启程
Owner 湖南金代科技发展有限公司