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Immobilized combined single cell oil culture and fermentation technology

A single-cell oil and joint culture technology, which is applied in the field of immobilized joint culture and fermentation of single-cell oil, can solve the problems of no culture technology and achieve the effects of cost reduction, high vitality and stability, and reduced vitality loss

Inactive Publication Date: 2019-03-19
BINZHOU POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no relatively mature cultivation technology in actual operation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Sterilize polyvinyl alcohol, sodium alginate, and chitin in the proportions of 28, 6, and 7 parts and mix them after being sterilized and cooled at 30°C. Add enzyme-producing microorganism A bacteria solution to prepare immobilized enzyme-producing cells, and soak in about 0.2% In the dialdehyde solution, keep the temperature at 30°C for 2 hours, wash with plenty of water repeatedly to remove the residual glutaraldehyde solution, wash with sterile water, inoculate the oil-producing oil-producing microorganism B28°C and mix for 96 hours to separate the oil-producing microorganisms Cells, the accumulation of fat is determined to reach 65±4% of the dry cell weight.

Embodiment 2

[0026] Sterilize polyvinyl alcohol, sodium alginate, and chitin in the proportions of 28, 6, and 7 parts and mix them after being sterilized and cooled at 30°C. Add enzyme-producing microorganism A bacteria solution to prepare immobilized enzyme-producing cells, and soak in about 0.2% In the dialdehyde solution, keep the temperature at 30°C for 2 hours, wash with plenty of water repeatedly to remove the residual glutaraldehyde solution, wash with sterile water, inoculate the oil-producing oil-producing microorganism B28°C and mix for 96 hours to separate the oil-producing microorganisms Cells, the accumulation of fat is determined to reach 66±3% of the dry cell weight.

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PUM

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Abstract

The invention discloses an immobilized combined single cell oil culture and fermentation technology. PVA polyvinyl alcohol, sodium alginate, calcium chloride, glutaraldehyde and chitin are involved. The invention further discloses specific culture steps of the immobilized combined single cell culture and fermentation technology, specifically comprising: (1) performing immobilized embedding on enzyme-producing bacteria A; and (2) performing mixed culture on the immobilized enzyme-producing microorganism A and an oil-producing microorganism B, and co-producing single cell oil. The immobilized combined single cell oil culture and fermentation technology utilizes oil-producing yeast to realize free culture, and can realize separation of an immobilized strain from the oil-producing yeast by simple filtration to reduce the influence of external factors on oil extraction.

Description

Technical field [0001] The invention relates to a single-cell lipid technology, in particular to an immobilized joint culture and fermentation single-cell lipid technology. Background technique [0002] The research on immobilization technology began in 1910, and the modern industrial utilization began in 1969 with the application of immobilized aminoacylase. With the implementation of immobilization technology, cells can be used repeatedly, product separation and extraction are easier, and continuity and automation can be achieved in production. The new functions and new applications of immobilization technology are rapidly expanding, and it is a wide range of research fields and applications. New research fields and new technologies with great prospects. [0003] The significance of using immobilization technology to carry out single-cell oil fermentation experiment for oil production is: on the one hand, the energy loss reduced by immobilization technology reduces costs; the en...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N11/10C12N11/08
CPCC12N11/08C12N11/10C12P7/6463
Inventor 赵春海李慧东李振清孙国荣
Owner BINZHOU POLYTECHNIC
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