Sterility stability analysis method for corn kernel male sterile gene msl-alb under wide genetic background and application
An analysis method, nuclear male technology, applied in the field of molecular breeding, seed engineering, and crop genetic breeding, can solve the problems of homogeneous cytoplasm, the degree of sterility easily affected by the genetic background, and the reduction of the utilization efficiency of germplasm resources.
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Embodiment 1
[0016] Example 1: Obtaining maize male sterile mutant material and 300 parts of maize inbred lines
[0017] The test material of the present invention is the normal fertile inbred line of maize ZmMs1 Sterile plants obtained by genetic mutations, showing complete male sterility, such as figure 1 As shown, its performance is specifically: the anthers are not exposed during the pollination period, the anthers become smaller, and no pollen grains are produced. The CMS material is named ms1-alb .
[0018] The 300 female maize inbred lines for testing were randomly selected from 1400 maize inbred lines in our laboratory, and the material names still used the original numbers.
Embodiment 2
[0019] Example 2: Identification of the population structure of 300 corn inbred lines
[0020] First, use the Illumina GoldenGate SNP Genotyping detection chip containing 3072 sites to analyze 300 inbred line materials, and then calculate the genetic distance (0-1.15) between different materials through the PHYLIP DNADIST program. According to the genetic distance calculation results, use PHYLIP NEIGHBOR software constructed a phylogenetic tree and classified 300 inbred lines. Such as figure 2 As shown, 300 maize inbred lines can be divided into 4 groups, representing better polymorphism.
Embodiment 3
[0021] Example 3: ms1-alb F 1 generation seed
[0022] Maize recessive nuclear male sterile mutants can only be preserved by sister crossing, that is, by crossing homozygous mutants and heterozygous mutants, and the theoretically obtained offspring fertile plants are all heterozygous ( ZmMs1 / ms1-alb ), the sterile plants were all homozygous ( ms1-alb / ms1-alb ), so the genotype can be judged by the phenotype. The present invention utilizes ms1-alb Fertile individual plants in sister cross populations ( ZmMs1 / ms1-alb ) to pollinate 300 inbred lines and get F 1 generation seeds.
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