High-sensitivity lipase kit

A high-sensitivity, kit-based technology, applied in the direction of color/spectral characteristic measurement, can solve the problems of preservative concentration interference, affecting the sensitivity of the kit, etc., and achieve high accuracy, increase the measurable linear range, and good specificity

Inactive Publication Date: 2019-03-19
闫玮钰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high concentration of preservatives added in this application will interfere to a certain extent and affect the sensitivity of the kit

Method used

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  • High-sensitivity lipase kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0039] Embodiment 1-5 a kind of high sensitivity lipase kit

[0040] Contains the following components:

[0041]

[0042]

[0043] Carry out accuracy performance evaluation with the kit of embodiment 1-5

[0044] Landox low and high value quality control products (1530, batch number 1045UN; 1532, batch number 801UE) were selected, and the BS-420 automatic biochemical analyzer was used for detection. The specific operation method was shown in Table 1. Finally, the measured value of LPS and the absorbance value of the blank can be read directly from the instrument. In order to reduce accidental errors, the average value was obtained by repeating the measurement 3 times, and the relative deviation between the measured value and the target value was calculated.

[0045] Table 1 embodiment 1-5 measures low value quality control (1530) accuracy comparison

[0046]

[0047] Table 2 embodiment 1-5 measures high-value quality control (1532) accuracy comparison

[0048]

...

Embodiment 6-8

[0050]Embodiment 6-8 a kind of high sensitivity lipase kit

[0051]

[0052]

[0053] Carry out accuracy performance evaluation with the kit of embodiment 6-8

[0054] Table 3 embodiment 6-8 measures low value quality control (1530) accuracy comparison

[0055]

[0056] Table 4 embodiment 6-8 measures high-value quality control (1532) accuracy comparison

[0057]

[0058] From the above results, it can be seen that the mean value measured by the kit of the present invention is within the target value range, and the relative deviation is less than 10%, indicating that the kit of the present invention has high accuracy.

Embodiment 9-11

[0059] Embodiment 9-11 A kind of high sensitivity lipase kit

[0060]

[0061]

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Abstract

The invention discloses a high-sensitivity lipase kit, which is prepared from the following components: a punching system at a concentration of 70 to 180 mmol / L and having a pH of 8.0 to 9.0, glyceroltrilaurate at a concentration of 90 to 350 mmol / L, oxidized coenzyme I at a concentration of 0.1 to 0.5 mmol / L, glycerol dehydrogenase at a concentration of 0.3 to 4 KU / L, an enzyme protective agentat a concentration of 4 to 12 g / L, a stabilizer at a concentration of 0.5 to 1.5 g / L, and a developing agent at a concentration of 0 .2 to 2.0 g / 100mL. The lipase kit disclosed by the invention does not contain a preservative, and the stability of the kit is obviously improved by controlling the mass ratio of the stabilizer to the enzyme protective agent, thereby avoiding interference caused by the presence of the preservative and improving the detection sensitivity; and the test accuracy is also significantly improved. The specific developing agent is added into the kit to enhance the surfacetension of a water-oil interface, thereby increasing the measurable linear range of a reagent; and furthermore, the detection result of the kit is accurate and high in repetitiveness.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a detection kit, in particular to a high-sensitivity lipase kit. Background technique [0002] Lipase (LPS) belongs to the carboxyl ester hydrolase class, which can gradually hydrolyze triglycerides into glycerol and fatty acids. It mainly comes from the pancreas, followed by the stomach and small intestine, and can hydrolyze a variety of glycerides containing long-chain (8-18 carbon chain) fatty acids. Lipase is synthesized in the pancreatic alveolar tissue of the pancreas and has high organ specificity. In normal blood, there is only a small amount of lipase, which is easily cleared by the kidneys. When the secretion of the pancreas is hypersecreted, the pancreatic duct is blocked, or the pancreas is damaged or necrotic, the lipase flows backward or directly into the blood, making the lipase in the blood active. Increase. When acute parotitis occurs, the secretion of L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 闫玮钰
Owner 闫玮钰
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