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A kind of explant pretreatment method before the rapid propagation of cat's whiskers aseptic short branches

A technology of cat's whiskers and explants, which is applied in the field of explant pretreatment before the rapid propagation of aseptic stubs of cat's whiskers, and can solve the problems of low efficiency of rapid propagation of seedlings, tissue culture pollution rate, reduction of pollution rate, etc. problem, to achieve the effect of good disinfection effect, low production cost and low pollution rate

Inactive Publication Date: 2021-06-01
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technology of rapid propagation of aseptic short shoots is an important in vitro micropropagation technology, but because the explants are usually directly taken from the budding stems that grow in the field and are polluted by a large number of bacteria and systemic fungi, resulting in The contamination rate of tissue culture is very high, so that the rapid propagation efficiency of seedlings is very low or even fails
Many scientific researchers use carbendazim, chlorothalonil, anti-virus alum, thiophanate-methyl, gentamicin, imidamide, tetracycline and other reagents alone or in a mixed solution to soak the explants as pretreatment before disinfection, or Adding Yipeiji bactericide on the solid medium, these treatments can reduce the pollution rate to a certain extent, but still cannot fundamentally solve the problem

Method used

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Effect test

Embodiment 1

[0017] A kind of explant processing method before the rapid propagation of cat's whiskers aseptic short shoots, comprising the following steps:

[0018] Step 1: Disinfect the cultivation substrate: use 350 times of 50% water-soluble disenium, spray evenly with 3 kg of dilution per square meter of culture substrate, and put it into a flowerpot for planting when the soil is slightly dry;

[0019] Step 2, pre-sterilize the airtight plastic greenhouse: 15 days before the transplanting of cat's whiskers, once every 5 days, use 0.4% imidamide for the first time, and 0.2% for the second time Mancozeb, the third time with 0.2% concentration of mancozeb liquid spray plastic greenhouse floor and around, disinfection treatment time is in the morning, airtight plastic greenhouse after spraying;

[0020] Step 3: Selection of transplanted seedlings and pruning and disinfection: Before transplanting seedlings and planting, select the vigorous-growing cat’s whiskers with full buds, no pests a...

Embodiment 2

[0025] A kind of explant processing method before the rapid propagation of cat's whiskers aseptic short shoots, comprising the following steps:

[0026] Step 1: Disinfect the cultivation substrate: use 350 times of 50% water-soluble disenium, spray evenly with 3 kg of dilution per square meter of culture substrate, and put it into flower pots for planting when the soil is slightly dry.

[0027] Step 2, pre-sterilize the airtight plastic greenhouse: 15 days before the transplanting of cat's whiskers, once every 5 days, use 0.4% imidamide for the first time, and 0.2% for the second time Mancozeb, the third time with 0.2% concentration of mancozeb liquid spray plastic greenhouse floor and around, disinfection treatment time is in the morning, airtight plastic greenhouse after spraying;

[0028] Step 3: Selection of transplanted seedlings and pruning and disinfection: Before transplanting seedlings and planting, select the vigorous-growing cat’s whiskers with full buds, no pests a...

Embodiment 3

[0033] A kind of explant processing method before the rapid propagation of cat's whiskers aseptic short shoots, comprising the following steps:

[0034] Step 1: Disinfect the cultivation substrate: use 350 times of 50% water-soluble disenium, spray evenly with 3 kg of dilution per square meter of culture substrate, and put it into a flowerpot for planting when the soil is slightly dry;

[0035] Step 2, pre-sterilize the airtight plastic greenhouse: 15 days before the transplanting of cat's whiskers, once every 5 days, use 0.4% imidamide for the first time, and 0.2% for the second time Mancozeb, the third time with 0.2% concentration of mancozeb liquid spray plastic greenhouse floor and around, disinfection treatment time is in the morning, airtight plastic greenhouse after spraying;

[0036] Step 3: Selection of transplanted seedlings and pruning and disinfection: Select the vigorous growth of cat’s whiskers with full buds, no pests and diseases, carefully dig up, shake off th...

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Abstract

The invention relates to a method for pretreatment of explants before the rapid propagation of aseptic short branches of cat's whiskers, comprising the following steps: (1) disinfecting the cultivation substrate; (2) pre-sterilizing the airtight plastic greenhouse; 3) Selection of transplanted seedlings and pruning and disinfection; (4) Colonization of transplanted seedlings; (5) Regular disinfection of seedlings; (6) Cutting new branches. The invention has the advantages of simple operation, good disinfection effect and low production cost, and can provide enough explants for industrialized rapid propagation; and the pollution rate of the method is low, the lowest being 2.0%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for pretreatment of explants before rapid propagation of aseptic short shoots of cat's whiskers. Background technique [0002] Clerodendranthus spicatus, a perennial herb of Lamiaceae, with erect stems, 1-1.5 meters high, quadrangular, light purple or white corolla, flower and fruit period from May to November; mainly distributed in Guangdong, Hainan, Guangxi South, southern Yunnan and Fujian; often grows in moist places under forests, sometimes on flat ground without shade. The upper part of cat's whisker grass is used as medicine, sweet and slightly bitter, with strong antibacterial and anti-inflammatory effects, which can reduce the destruction of renal tubules, reduce the structural changes of glomerular cells, increase the number of intact glomeruli, and protect and improve the damage of renal tissue , can increase glomerular filtration and renal circu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/00
CPCA01G22/00
Inventor 朱鹏锦庞新华韦俏宇梁春严霖吕平周全光王丽萍
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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