Malachite green detection method in a kind of aquatic products

Abstract

The invention discloses a detection method for malachite green in aquatic products. The detection method comprises experiment steps of preparing a magnetic molecularly imprinted polymer, establishinga method based on an aptamer and ferroferric oxide mimic enzyme catalysis and the like. According to the detection method, firstly, a dopamine polymerization method is used for preparing a malachite green (MG) magnetic molecularly imprinted polymer (MG-MMIPs); trace MG in a sample is adsorbed by utilizing the MG-MMIPs, so that the aims of enriching a target object and removing a complicated mediumare realized; then after enriched MG is desorbed, a detection method of the aptamer and ferroferric oxide mimic enzyme catalysis is used for detecting. The method has the sensitivity of 5 nmol L<-1>and the lowest detection limit reaches 3.6 nmol L<-1>. A specific experiment aiming at two structural analogues (including brilliant green and crystal violet) of MG and other bacteriostatic drugs (including sulfaguanidine, sulphanilamide, oxytetracycline hydrochloride and chloramphenicol) shows that the method has good selectivity in MG detection.

Description

technical field [0001] The invention relates to a detection method of malachite green, in particular to a detection method of malachite green in aquatic products. Background technique [0002] Malachite green (Malachite green, MG) is a toxic triphenylmethane substance, also known as base block green, basic malachite green, malachite green, basic green, Chinese green or aniline green, with metallic luster dark green crystalline solid. Malachite green can also be used as a fungicide, which has a good effect on preventing the growth of mold in fish eggs and the secondary pollution of fungi. However, malachite green has the disadvantages of high toxicity, high residue, high carcinogenicity and mutagenesis. Because of its low price and remarkable bactericidal effect, and there is no suitable substitute, it is still used by unscrupulous merchants. Because it can achieve a good bactericidal effect with a small amount of addition, it brings certain difficulties to the detection o...

AI Technical Summary

Problems solved by technology

However, these methods are cumbersome, complicated, and time-consuming in the sample pretreatment process, and the equipment is expensive and the detection cost is high. Not only does it need to consume a lot of solvents and time, but it is also easy to cause secondary pollution, which affects the accuracy of the test results.

The enzyme-linked immunosorbent method needs to prepare antibodies, the operation is complicated, and it is easily affected by environmental conditions such as pH value and temperature, and the detection conditions are harsh.

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