Preparation method and application of photosensitive nanocarrier for carrying melittin
A nano-carrier, melittin technology, which can be used in preparations for in vivo experiments, medical preparations containing active ingredients, and pharmaceutical formulas, can solve problems such as insufficient intake of nanoparticles, inhibit tumor growth, and enhance cure. effect, good biocompatibility and biodegradability
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[0035] A method for preparing a photosensitive nanocarrier carrying melittin, comprising the following steps:
[0036] 1) Preparation of nano-alumina particles: Al 3+ Dissolve the metal salt in water, add alkaline reagents drop by drop, adjust the pH to reach an alkaline environment, carry out oxidation-reduction reaction under high temperature and high pressure conditions, after the reaction is complete, successively centrifuge and wash to obtain nano-alumina particles;
[0037] 2) Preparation of biocompatible nano-metal particles: ultrasonically disperse the nano-alumina particles obtained in the step 1) in water, add bovine serum albumin BSA, and dialyze after the reaction is complete to obtain biocompatible nano-metal particles Al 2 o 3 @BSA; In this step, the principle of the reaction between nano-alumina particles and bovine serum albumin is because BSA is a negatively charged protein, while nano-alumina is a nanoparticle with a higher positive charge, through the inter...
Embodiment 1
[0051] Step 1: In a 50mL flask, add 20mL of deionized water, and dissolve 3.7mol of Al 3+ Put the solution into deionized water, then take 238uL of ethylenediamine solution and add it dropwise, stir at room temperature for 30min, then add the mixed solution into the hydrothermal reaction kettle, keep the reaction at 200°C for 8 hours, and cool the reacted solution to room temperature naturally , were separated by centrifugation, washed with ethanol and deionized water in turn to remove residual solvent, and the purified nanoparticles were stored at 4 °C.
[0052] Step 2: Take 100 mg of nanoparticles prepared in Step 1, ultrasonically disperse them in deionized water for 2 hours, then immediately add 2 mL of BSA solution (0.1 mmol / L) under magnetic stirring conditions, and stir for another 24 hours at room temperature. After the reaction, The mixed solution was placed in a dialysis bag and placed in deionized water for dialysis, and the deionized water was changed every 4 hours...
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