Preparation method and application of photosensitive nanocarrier for carrying melittin

A nano-carrier, melittin technology, which can be used in preparations for in vivo experiments, medical preparations containing active ingredients, and pharmaceutical formulas, can solve problems such as insufficient intake of nanoparticles, inhibit tumor growth, and enhance cure. effect, good biocompatibility and biodegradability

Inactive Publication Date: 2019-03-26
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a method for preparing a photosensitive nanocarrier carrying melittin, which can solve th

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  • Preparation method and application of photosensitive nanocarrier for carrying melittin
  • Preparation method and application of photosensitive nanocarrier for carrying melittin
  • Preparation method and application of photosensitive nanocarrier for carrying melittin

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preparation example Construction

[0035] A method for preparing a photosensitive nanocarrier carrying melittin, comprising the following steps:

[0036] 1) Preparation of nano-alumina particles: Al 3+ Dissolve the metal salt in water, add alkaline reagents drop by drop, adjust the pH to reach an alkaline environment, carry out oxidation-reduction reaction under high temperature and high pressure conditions, after the reaction is complete, successively centrifuge and wash to obtain nano-alumina particles;

[0037] 2) Preparation of biocompatible nano-metal particles: ultrasonically disperse the nano-alumina particles obtained in the step 1) in water, add bovine serum albumin BSA, and dialyze after the reaction is complete to obtain biocompatible nano-metal particles Al 2 o 3 @BSA; In this step, the principle of the reaction between nano-alumina particles and bovine serum albumin is because BSA is a negatively charged protein, while nano-alumina is a nanoparticle with a higher positive charge, through the inter...

Embodiment 1

[0051] Step 1: In a 50mL flask, add 20mL of deionized water, and dissolve 3.7mol of Al 3+ Put the solution into deionized water, then take 238uL of ethylenediamine solution and add it dropwise, stir at room temperature for 30min, then add the mixed solution into the hydrothermal reaction kettle, keep the reaction at 200°C for 8 hours, and cool the reacted solution to room temperature naturally , were separated by centrifugation, washed with ethanol and deionized water in turn to remove residual solvent, and the purified nanoparticles were stored at 4 °C.

[0052] Step 2: Take 100 mg of nanoparticles prepared in Step 1, ultrasonically disperse them in deionized water for 2 hours, then immediately add 2 mL of BSA solution (0.1 mmol / L) under magnetic stirring conditions, and stir for another 24 hours at room temperature. After the reaction, The mixed solution was placed in a dialysis bag and placed in deionized water for dialysis, and the deionized water was changed every 4 hours...

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Abstract

The invention provides a preparation method of a photosensitive nanocarrier for carrying melittin. The preparation method comprises the following steps that (1) nano-alumina particles are prepared; (2) biocompatible nano-metal particles are prepared; (3) the nanocarrier for carrying the melittin is prepared; and (4) a photosensitizer is added to obtain photosensitive nanoparticles, namely Al2O3@BSA@Melittin@Ce6, for carrying the melittin. The photosensitive nanocarrier, namely Al2O3@BSA@Melittin@Ce6, for carrying the melittin is applied to preparation of anticancer drugs. According to the preparation method of the photosensitive nanocarrier for carrying the melittin, the preparation problem of the photosensitive nanocarriers for carrying the melittin can be solved, and meanwhile, the technical problem that normal cells take in nanoparticles insufficiently is solved.

Description

technical field [0001] The invention relates to the technical field of bio-nano medicine, in particular to a preparation method and application of a photosensitive nano-carrier carrying melittin. Background technique [0002] Melittin (GIGAVLKVLTTGLPALISWIKRKRQQ) is an amphipathic α-helical polypeptide and is the main active ingredient in bee venom, accounting for 40%-60% of its dry weight. Melittin has high activity, has broad-spectrum antibacterial function, can play its role in a very short time, and its efficiency is hundreds of times that of ordinary antibiotics. Melittin is highly destructive to cell membrane structures, including plasma membranes and membrane structures of some intracellular organelles. Its mechanism of action is mainly to embed into the membrane structure through its amphiphilic structure, and then punch holes in the membrane, causing the membrane structure to rupture, and the changes in the internal and external environment of the membrane eventual...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K9/51A61K47/42A61K41/00A61K49/00A61P35/00
CPCA61K9/5169A61K38/1767A61K41/0071A61K49/0036A61K49/0093A61P35/00A61K2300/00
Inventor 杨坤禹金红林徐祖顺刘豪杰胡彦万超
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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