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Preparation method and application of nanocomposite material for inhibiting postoperative tumor recurrence

A nano-composite material and tumor technology, which is applied in the direction of anti-tumor drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. It can solve the problems of unsatisfactory EPR effect and achieve the purpose of retaining the activity of nano-anticancer drugs , Inhibition of tumor recurrence, little effect

Active Publication Date: 2019-03-29
NANJING UNIV OF POSTS & TELECOMM
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Problems solved by technology

However, the EPR effect has great individual differences, and there will be differences between different tumors, different patients, and different parts of the same patient (Science Translational Medicine, 2015.7 (314): p.314ra183-314ra183), and the EPR effect has great influence on the size of nanoparticles. There are certain requirements, the EPR effect of nanoparticles with too large or too small size is not ideal

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  • Preparation method and application of nanocomposite material for inhibiting postoperative tumor recurrence
  • Preparation method and application of nanocomposite material for inhibiting postoperative tumor recurrence
  • Preparation method and application of nanocomposite material for inhibiting postoperative tumor recurrence

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preparation example Construction

[0032] A preparation method and application of a nanocomposite material for inhibiting postoperative tumor recurrence, the method comprising the following steps: (a) the curve of the fluorescence intensity of Dox as a function of concentration; (b) Dox is loaded in MSN; (c) Calculation of loading ratio; (d) DMSN mixed with PCL and hot-pressed; (e) Characterization of the prepared nanocomposite; (f) Determination of in vitro drug release profile; (g) In vitro cytotoxicity test; (h) In vivo stability and degradability tests.

[0033] Specifically, a method for preparing a nanocomposite material for inhibiting postoperative tumor recurrence, the method includes the following steps:

[0034] S1: First prepare Dox solutions with different concentrations, and use a fluorescence spectrophotometer to measure the fluorescence intensity of each solution at the emission wavelength, thereby obtaining the variation curve of the Dox fluorescence intensity at the emission wavelength with con...

Embodiment 1

[0049] 1. First prepare Dox solutions with different concentrations. The specific Dox solutions are: 0.025 μg / mL, 0.05 μg / mL, 0.1 μg / mL, 0.2 μg / mL, 0.4 μg / mL, 0.8 μg / mL, 1.6 μg / mL, Using a fluorescence spectrophotometer, the excitation wavelength is 485nm, the emission wavelength is 575nm, the fluorescence intensity of each solution at 575nm is measured, and the curve of Dox fluorescence intensity at 575nm with concentration is drawn.

[0050] 2. Take 6mL Dox mother liquor with a concentration of about 0.5mg / mL and mix it with 5mg MSN. Before mixing, measure the fluorescence intensity of the mother liquor at 575nm, and calculate the precise concentration C of the mother liquor through the above curve. 母 . After mixing evenly, stir for 12 hours under the condition of avoiding light, and the rotation speed is 500 rpm.

[0051] 3. After 12 hours, take out the solution and centrifuge, the speed of the centrifuge is: 8000rpm, and the centrifugation time: 20min. After the centrifu...

Embodiment 2

[0058] 1. First prepare Dox solutions with different concentrations. The specific Dox solutions are: 0.025 μg / mL, 0.05 μg / mL, 0.1 μg / mL, 0.2 μg / mL, 0.4 μg / mL, 0.8 μg / mL, 1.6 μg / mL, Using a fluorescence spectrophotometer, the excitation wavelength is 485nm, the emission wavelength is 575nm, the fluorescence intensity of each solution at 575nm is measured, and the curve of Dox fluorescence intensity at 575nm with concentration is drawn.

[0059]2. Take 6mL Dox mother liquor with a concentration of about 0.5mg / mL and mix it with 5mg MSN. Before mixing, measure the fluorescence intensity of the mother liquor at 575nm, and calculate the precise concentration C of the mother liquor through the above curve. 母 . After mixing evenly, stir for 12 h under the condition of avoiding light (rotating speed 500 rpm).

[0060] 3. After 12 hours, take out the solution and centrifuge (speed: 8000rpm, time: 20min). After the centrifugation was completed, the supernatant was taken out, and the p...

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Abstract

The invention discloses a preparation method and application of a nanocomposite material for inhibiting postoperative tumor recurrence. The method has the advantages of simple preparation, uniform distribution of nano-drugs, little influence on loaded drug activity and the like. At the same time, the prepared nanocomposite material has good mechanical properties and biocompatibility, and can be implanted in a body for long-term drug sustained release. Polycaprolactone is used as a bulk material, mesoporous silica nanoparticles are used as a drug carrier, doxorubicin is used as a chemotherapeutic drug, the biodegradable nanocomposite material doped with inorganic nano-drugs is prepared by a solvent-free low-temperature ball milling method, doped nanoparticles are uniformly distributed in the bulk polymer material, and the nanocomposite material has mechanical properties of the bulk polymer material. A new method is provided for preparing the nanocomposite material and realizing implantable localized delivery of anticancer drugs, and the nanocomposite material is hopeful to be applied to inhibition of postoperative tumor recurrence.

Description

technical field [0001] The invention relates to a preparation method and application of a nanocomposite material for inhibiting postoperative tumor recurrence, and belongs to the technical fields of nanocomposite material preparation and biomedical engineering. Background technique [0002] In a report published by the National Cancer Center in 2016, the incidence and death of malignant tumors in China in 2012 were analyzed (China Tumor, 2016.25(1): p.1-8), and the data show that malignant tumors are still a threat to human health and life. Primary critical illness. In China, it has become an indisputable fact that the incidence of cancer continues to rise. According to the latest cancer data in China in 2017, about 10,000 people are diagnosed with cancer every day in China. According to statistics, my country spends as much as 80 billion yuan on cancer treatment every year. How to achieve effective treatment of tumors, alleviate patients' pain and improve their quality of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K47/34A61K47/04A61P35/00
CPCA61K31/704A61K47/02A61K47/34A61P35/00
Inventor 高宇汪联辉肖华新翁丽星王思雨王波任丽丽
Owner NANJING UNIV OF POSTS & TELECOMM
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