Extraction method of selenium polysaccharide from pear pulp

An extraction method and technology of selenium polysaccharides are applied in the field of plant physiology to achieve the effects of easy industrial promotion, less impurities and good absorption effect

Active Publication Date: 2019-03-29
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the extraction and separation process of pear pulp selenium polysaccharides, especially the research of foreign scholars in this area has not been reported

Method used

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  • Extraction method of selenium polysaccharide from pear pulp
  • Extraction method of selenium polysaccharide from pear pulp
  • Extraction method of selenium polysaccharide from pear pulp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 A kind of extraction method of pear pulp selenium polysaccharide

[0030] Such as figure 1 Shown, a kind of extraction method of pear pulp selenium polysaccharide comprises the following steps:

[0031] 1) Pre-treatment: select Dangshansu pears with uniform fruit size as the test material, wash them with tap water first, then rinse them repeatedly with distilled water, remove the peel and core, keep the pulp, chop and dry it, and grind it into dry powder with a grinder for later use;

[0032] 2) Water extraction: Weigh 5.0g of dry powder sample into a three-necked bottle, add 40ml of pure water into the three-necked bottle into the reflux device, reach 100°C for constant temperature extraction for 1 hour, add a centrifuge tube and centrifuge at 4000r / min in a centrifuge for 15 minutes Take out the supernatant and residue 1, add 40ml of pure water to the residue 1, reflux leaching in a boiling water bath for 2 times to obtain the supernatant and residue 2, ...

Embodiment 2

[0037] Embodiment 2 A kind of extraction method of pear pulp selenium polysaccharide

[0038] 1) Pre-treatment: select Dangshansu pears with uniform fruit size as the test material, wash them with tap water first, then rinse them repeatedly with distilled water, remove the peel and core, keep the pulp, chop and dry it, and grind it into dry powder with a grinder for later use;

[0039] 2) Water extraction: Take 5.0g of dry powder sample in a three-necked bottle, add 60ml of pure water into the three-necked bottle into the reflux device, reach 100°C for constant temperature extraction for 1 hour, add a centrifuge tube and centrifuge at 4000r / min in a centrifuge for 15 minutes, then take Take out the supernatant and residue 1, then add 60ml of water to the residue 1 after centrifugation, and reflux extraction in a boiling water bath for 2 times to obtain the supernatant and residue 2, and combine the obtained supernatant 3 times, that is Water-soluble selenium polysaccharide ext...

Embodiment 3

[0044] Embodiment 3 A kind of extraction method of pear pulp selenium polysaccharide

[0045] 1) Pre-treatment: select Dangshansu pears with uniform fruit size as the test material, wash them with tap water first, then rinse them repeatedly with distilled water, remove the peel and core, keep the pulp, chop and dry it, and grind it into dry powder with a grinder for later use;

[0046] 2) Water extraction: Take 5.0g of dry powder sample in a three-necked bottle, add 40ml of pure water into the three-necked bottle, enter the reflux device, reach 100°C for constant temperature extraction for 1 hour, add a centrifuge tube and centrifuge at 4000r / min in a centrifuge for 15 minutes Take out the supernatant and residue 1, then add 40ml of water to the residue 1 after centrifugation, and reflux extraction in a boiling water bath for 2 times to obtain the supernatant and residue 2, and combine the obtained supernatant for 3 times, namely It is the water-soluble selenium polysaccharide...

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Abstract

The invention discloses an extraction method of selenium polysaccharide from pear pulp, which includes: drying and pulverizing a sample, and performing ultrasonic assistance-combined solvent continuous extraction method for extracting the selenium polysaccharide, wherein the sample is successively extracted with distilled water, a HCl solution or an alkali solution to obtain the selenium polysaccharides from the pear pulp, in different dissolubilities. In the invention, for the first time, the ultrasonic assistance extraction is combined, thus issuing the novel selenium polysaccharide extraction method - ultrasonic assistance-combined solvent continuous extraction method. In the method, the distilled water, HCl solution and NaOH solution are successively used for extracting the water soluble, acid soluble and alkali soluble selenium polysaccharides under the ultrasonic assistance. The method is high effective in selenium polysaccharide extraction and has extensive market promotion value.

Description

technical field [0001] The invention belongs to the field of plant physiology, and in particular relates to a method for extracting pear pulp selenium polysaccharide. Background technique [0002] Selenium is an essential trace element for organisms, and the selenium ingested by humans or animals comes directly or indirectly from plants. Selenium is recognized as the "King of Anticancer" in the world, and it is also one of the indispensable trace elements for the human body. Selenium has important functions such as anti-oxidation, anti-cancer and anti-cancer, detoxification and detoxification, and improving the body's immunity. Since many diseases such as Keshan disease, atherosclerosis, and cataract formation are related to active free radicals, for example, it can work together with vitamin E to prevent the attack of oxygen free radicals on cell membranes, so a certain amount of selenium can be added to Eliminate free radicals and cholesterol in the body and prevent the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王纪忠杨美玲徐海亮王云鹏潘国庆
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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