General-purpose nanochip for mass spectrometry and preparation method and application thereof

Abstract

The invention discloses a general-purpose nanochip for mass spectrometry, and relates to the technical field of mass spectrometry. The host material of the nanochip is silicon-based semiconductor material, array spotted holes are distributed at the surface of the host material, the inner surfaces of the spotted holes are nanostructures; the surface of the host material has regional hydrophobic modification, the internal portions of the array spotted holes are hydrophilic zones, and the outer portions of the array spotted holes are hydrophobic zones; or the internal portions of the array spotted holes are hydrophobic zones, and the outer portions of the array spotted holes are hydrophilic zones. The present invention further discloses a preparation method and a clinical application of the nanochip. The nanostructures can be used to extract the molecules at the surface of the sample of the biological tissue sample to be tested to improve the laser energy absorption and utilization so asto improve the ionization efficiency and enhance the mass spectrometry signals. The general-purpose nanochip can be widely applied to the field of clinical testing.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry, in particular to a general-purpose nanochip for mass spectrometry and its preparation method and application. Background technique [0002] The principle of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is to mix the matrix compound that can absorb laser energy with the sample to form a co-crystal, and the absorbed energy of the matrix is ​​transferred to the sample to make the sample to be tested In ionization, ions are accelerated in an electric field and fly through the flight tube, and are detected with different mass-to-charge ratios (m / z) depending on the flight time to the detector. As a soft ionization technique, MALDI-TOF is an important detection method in drug screening and clinical diagnosis, and can be applied to protein, peptide, microorganism, SNP gene detection, etc. [0003] The sample target plate is one of the most import...

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Problems solved by technology

[0003] The sample target plate is one of the most important consumables for MALDI-TOF mass spectrometers. Commercial common target plates are made of stainless steel. Although they can be reused, the existing problems are: (1) Organic reagents such as acetone, acetonitrile, and ethanol are required after use Ultrasonic cleaning, the process is cumbersome; (2) It is easy to cause sample residue and scratches on the surface of the target plate, resulting in cross-contamination, affecting the flatness of the target plate and matrix crystallization effect, thereby affecting the accuracy of clinical sample identification; (3) Detection of small molecule samples When detecting nucleic acid samples, the crystallization of the matrix is ​​uneven, the signal-to-noise ratio is low, and mass spectrometry imaging of tissue samples cannot be directly performed, and the application range is narrow; (4) The preparation cost is high, and it is difficult to detect in high-throughput clinical mass spectrometry. Large-scale promotion in the field

Disposable target plates that use metals as conductive materials to dope or surface-modify nanomaterials, the production process is complicated, the cost is further increased, the detection sample is single and the application field is limited

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