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Method for determining concentration of furosemide in plasma by using liquid chromatography-mass spectrometry

A liquid mass spectrometry and furosemide technology, applied in the field of medicine, can solve the problems of furosemide determination methods and literature reports, etc., and achieve the effect of simple pretreatment method, good peak shape and strong specificity

Inactive Publication Date: 2019-03-29
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no literature report on the determination method of furosemide

Method used

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  • Method for determining concentration of furosemide in plasma by using liquid chromatography-mass spectrometry
  • Method for determining concentration of furosemide in plasma by using liquid chromatography-mass spectrometry
  • Method for determining concentration of furosemide in plasma by using liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Example: Human K 2 Determination of Furosemide Concentration in EDTA Plasma

[0034] 1. Experimental materials and analytical equipment

[0035] Furosemide (analyte): China National Institutes for Food and Drug Control or equivalent or higher grade standard

[0036] Furosemide-d5 (internal standard): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0037] The reagents used are shown in Table 1 below:

[0038] Table 1 Reagent Details

[0039] Reagent name

level

manufacturer

Acetonitrile

HPLC

J. T. Baker

Methanol

HPLC

J. T. Baker

formic acid

ACS

Adamas

Ammonium acetate

HPLC

J. T. Baker

[0040] Note: Reagents of the same grade or higher can also be used

[0041] The analytical equipment used is shown in Table 2 below:

[0042] Table 2 Details of equipment used

[0043] components

type

manufacturer

Binary pump (binary pump)

AC Pump

A...

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Abstract

The invention discloses a method for determining the concentration of furosemide in plasma by using liquid chromatography-mass spectrometry. The concentration of furosemide in the plasma is determinedby using a liquid chromatography-mass spectrometry system; taking a sample to be tested first, and adding a certain amount of mixed organic solvent for extraction twice; after pretreatment, performing chromatographic column separation, and then detecting by using a mass spectrometry detector. The method for determining the concentration of furosemide in the plasma by using the liquid chromatography-mass spectrometry disclosed by the invention has the advantages of rapid, accurate, high sensitivity and simple operation, and provides a basis for determining the blood concentration of furosemide; and the plasma standard curve linear range of the method is 10 to 2000 ng / mL, and the intra-assay and inter-assay precisions RSDs are less than + / -15%, which is suitable for determining the concentration of furosemide in the plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for determining a drug, in particular to a method for determining the concentration of furosemide in blood plasma by liquid mass spectrometry. Background technique [0002] Furosemide is a known compound with the following structure: [0003] [0004] Furosemide, also known as furosemide, chemical name: 2-[(2-furylmethyl)amino]-5-(sulfamoyl)-4-chlorobenzoic acid, is a high-efficiency diuretic that acts on the ascending limb of the loop of Heinz. It is clinically used in the clinical situation where diuretics are not effective and diuretics are urgently needed. It can be used to treat congestive heart failure, liver cirrhosis, and edema caused by kidney disease, hypertension, acute pulmonary edema or cerebral edema, and accelerate the elimination of some drugs through the kidneys. excretion of poisons. [0005] At present, there is no literature report on the deter...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 林明弘
Owner 徐州立顺康达医药科技有限公司
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