Method for enlarging genetic variation range of brassica napus

Abstract

The invention belongs to the technical field of biological breeding, and discloses a method for enlarging a genetic variation range of Brassica napus. The method comprises the following steps: interspecifically hybridizing brassica napus and brassica juncea, pollinating, and then rescuing an immature embryo to obtain a triploid hybrid clone; screening hybrid plants with trace pollen for selfing and embryo rescuing to establish clone population S1; performing the selfing for the clone S1 to obtain S2 population, and performing selfing for S2 to obtain S3; performing selfing for S3 to obtain S4,thus obtaining brassica napus; and if S1 has no plant meeting the requirements, continuously performing the embryo rescuing for S1, and selecting the clone of the similar fertile brassica napus for selfing. According to the method of the invention, the hybrid of the brassica napus and natural brassica napus is utilized to enlarge the variation range of the planting resources of the Brassica napus, the hybrid progeny constructs a recombinant inbred line population or microspore culture to construct a DH population by virtue of continuous selfing, and materials with relatively stable heredity and excellent agricultural traits and quality traitsare screened from the population for genetic breeding.

Description

technical field [0001] The invention belongs to the technical field of biological breeding, in particular to a method for widening the genetic variation range of Brassica napus. Background technique [0002] At present, the commonly used prior art in the industry is as follows: rapeseed is one of the main oil crops in the world, and it is also an important edible oil source, protein feed, industrial raw material and nectar source plant. my country is one of the first countries to cultivate rapeseed, but in the past it was mainly the cultivation of cabbage-type rapeseed. In the 1930s, Brassica napus (AACC) began to be introduced and cultivated from North Korea, Japan and the United Kingdom. Cabbage-type rape has become one of the most important oil crops in my country. With the improvement of people's material living standards and the continuous deepening of rapeseed breeding research, people have put forward higher requirements for rapeseed breeding goals, and breeding high...

AI Technical Summary

Problems solved by technology

[0004] (1) The existing artificially synthesized rapeseed has the difficulty of hybridization compatibility, and the progress of genetic stabilization of the hybrid offspring (artificially synthesized Brassica napus) is relatively slow; it is difficult to select materials with good properties suitable for oil use for production; Breeding has poor compatibility of hybrid offspring, and it is difficult to obtain a new type of Brassica napus with stable genetics and excellent traits, and the genetic base is relatively narrow

[0005] (2) Existing use of Brassica oleracea and Brassica napus hybrids to introduce genomes from Brassica oleracea and Chinese cabbage respectively, cannot obtain more excellent traits for production

[0006] (3) The existing innovation sources of Brassica napus germplasm resources are not wide enough, the range of genetic variation is small, and it is not easy to screen for new Brassica napus with agronomic traits for production

[0007] (4) At present, the excellent traits of the three species of Brassica oleracea, Brassica napus, and Brassica napus are concentrated, and the creation of new Brassica napus requires two interspecific hybridizations and two interspecific hybrid offspring selections, resulting in hybrid incompatibility Or the offspring of hybrids are sterile, which takes a long time, complicated procedures, and heavy workload

Images