Organ chip and method for evaluating interaction of nanoparticles and pulmonary surfactant layer based on organ chip technology

Abstract

The invention provides an organ chip and a method for evaluating the interaction of nanoparticles and a pulmonary surfactant layer based on an organ chip technology. The chip consists of four layers and is formed by bonding and sealing a topmost layer of PDMS, a second layer of a hydrophilic treated PET film, a third layer of PDMS and a fourth layer of a glass sheet from top to bottom; the chip includes an extracellular matrix inlet channel, a nanoparticle inlet channel, a waste liquid outlet channel, a gas supply channel, a pulmonary epithelial cell culture chamber, an aspirating chamber, andan aspirating channel. The chip is inoculated with pulmonary epithelial cells, and the pulmonary surfactant layer is produced by the pulmonary epithelial cells after culture is performed for a certain period of time; nanoparticles are added into the pulmonary epithelial cell culture chamber, and an evaluation model of the interaction between the nanoparticles and the pulmonary surfactant in vitrois established. The evaluation model of the interaction between the nanoparticles and the pulmonary surfactant based on the chip not only can simulate the effect of the nanoparticles on the pulmonarysurfactant layer, can monitor the layer in real time, but also can be used for evaluating the damage of drugs to the pulmonary epithelial cells.

Description

technical field [0001] The invention relates to an organ chip and a method for evaluating the interaction between nano particle lung surfactant layers based on the organ chip technology. Background technique [0002] Due to the rapid development of nanotechnology, nanoparticles are now widely used in different fields of normal life. For example, nanoparticles are used as carriers for drugs or as diagnostic tools in medicine and research. The lung is the main organ of the respiratory system. Nanoparticles administered from the lung need to cross the biological interface and cell barrier in the alveoli, so as to first contact the pulmonary surfactant layer on the upper layer of the lung epithelial cell membrane. The deposition of nanoscale particles in the alveoli may cause an uncontrolled inflammatory response in the lungs, affect the activity of alveolar epithelial cells, and affect the secretion of proteins responsible for regulating lung surface tension or participating i...

AI Technical Summary

Problems solved by technology

[0005] However, the use of organ chips to evaluate the toxicity of nano-drug particles to the lung epithelium to reflect the role of nanoparticles on the lung surfactant layer and lung epithelial cells in the respiratory process is still a blank field in lung injury research.

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