Organ chip and method for evaluating interaction of nanoparticles and pulmonary surfactant layer based on organ chip technology

An organ chip and nanoparticle technology, applied in stress-stimulated microbial growth methods, biochemical equipment and methods, biochemical instruments, etc., to achieve the effect of complete functions and simple operation

Inactive Publication Date: 2019-04-02
SHANGHAI NAT ENG RES CENT FORNANOTECH
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the use of organ chips to evaluate the toxicity of nano-drug particles to the lung epithelium to reflect the role of nanop

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Organ chip and method for evaluating interaction of nanoparticles and pulmonary surfactant layer based on organ chip technology
  • Organ chip and method for evaluating interaction of nanoparticles and pulmonary surfactant layer based on organ chip technology
  • Organ chip and method for evaluating interaction of nanoparticles and pulmonary surfactant layer based on organ chip technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] .An organ chip, consisting of four layers, from top to bottom are the first and third layers of PDMS layers I and III, the second layer of hydrophilic membrane II, and the fourth layer of glass sheet IV bonded and sealed. The first PDMS layer is formed, including extracellular matrix inlet channel 1, nanoparticle inlet channel 2, waste liquid outlet channel 3, air supply channel 4, and lung epithelial cell culture chamber 5; the third layer PDMS layer III is provided with an air pumping chamber 6 , communicated with the pumping channel 7 on the first PDMS layer I; where,

[0047]The two ends of the lung epithelial cell culture chamber (5) are connected to the Y-shaped channel formed by the intersection of the extracellular matrix inlet channel 1 and the nanoparticle inlet channel 2 and the waste liquid outlet channel 3, and the upper surface of the lung epithelial cell culture chamber 5 is connected to the air supply channel. 4 connected.

[0048] In this embodiment, t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention provides an organ chip and a method for evaluating the interaction of nanoparticles and a pulmonary surfactant layer based on an organ chip technology. The chip consists of four layers and is formed by bonding and sealing a topmost layer of PDMS, a second layer of a hydrophilic treated PET film, a third layer of PDMS and a fourth layer of a glass sheet from top to bottom; the chip includes an extracellular matrix inlet channel, a nanoparticle inlet channel, a waste liquid outlet channel, a gas supply channel, a pulmonary epithelial cell culture chamber, an aspirating chamber, andan aspirating channel. The chip is inoculated with pulmonary epithelial cells, and the pulmonary surfactant layer is produced by the pulmonary epithelial cells after culture is performed for a certain period of time; nanoparticles are added into the pulmonary epithelial cell culture chamber, and an evaluation model of the interaction between the nanoparticles and the pulmonary surfactant in vitrois established. The evaluation model of the interaction between the nanoparticles and the pulmonary surfactant based on the chip not only can simulate the effect of the nanoparticles on the pulmonarysurfactant layer, can monitor the layer in real time, but also can be used for evaluating the damage of drugs to the pulmonary epithelial cells.

Description

technical field [0001] The invention relates to an organ chip and a method for evaluating the interaction between nano particle lung surfactant layers based on the organ chip technology. Background technique [0002] Due to the rapid development of nanotechnology, nanoparticles are now widely used in different fields of normal life. For example, nanoparticles are used as carriers for drugs or as diagnostic tools in medicine and research. The lung is the main organ of the respiratory system. Nanoparticles administered from the lung need to cross the biological interface and cell barrier in the alveoli, so as to first contact the pulmonary surfactant layer on the upper layer of the lung epithelial cell membrane. The deposition of nanoscale particles in the alveoli may cause an uncontrolled inflammatory response in the lungs, affect the activity of alveolar epithelial cells, and affect the secretion of proteins responsible for regulating lung surface tension or participating i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M1/00C12M1/34C12M1/36
CPCC12M23/16C12M35/04C12M41/36C12M41/48
Inventor 何丹农朱君张心依朱竞尧刘睿金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap