Electronic identity database and detection method of pesticide compounds in edible agricultural products based on GC-Q-Orbitrap

An electronic identity and electronic identity card technology, applied in the field of electronic identity database and detection of pesticide compounds in edible agricultural products based on GC-Q-Orbitrap, to save resources, improve accuracy and operability, and increase accuracy and reliability.

Active Publication Date: 2019-04-02
CHINESE ACAD OF INSPECTION & QUARANTINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem that simultaneous rapid detection of multiple pesticides cannot be realized in current pesticide residue screening techniques and methods, and develops a GC-Q-Orbitrap-based electronic identity database and detection method of pesticide compounds for edible agricultural products, which realizes the needless Standard control can quickly screen and detect more than 600 pesticide residues in agricultural products at the same time, which meets the urgent need for high-throughput rapid detection of pesticide residues in agricultural products

Method used

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  • Electronic identity database and detection method of pesticide compounds in edible agricultural products based on GC-Q-Orbitrap
  • Electronic identity database and detection method of pesticide compounds in edible agricultural products based on GC-Q-Orbitrap
  • Electronic identity database and detection method of pesticide compounds in edible agricultural products based on GC-Q-Orbitrap

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Implementation examples of GC-Q-Orbitrap screening and confirmation technology for more than 600 pesticides in cabbage, including the following steps;

[0093] 1. Specific steps of sample pretreatment technology:

[0094] 1.1 Take the edible part of the head cabbage sample, chop it up, mix it evenly, seal it, and mark it;

[0095] 1.2 Weigh 10g of cabbage sample (accurate to 0.01g), add 40mL of 1% acetic acid acetonitrile into a 100mL centrifuge tube, use a high-speed homogenizer at 10000r / min, homogenize and extract for 1min, add 1g of sodium chloride, 4g of no Magnesium sulfate in water, shaken for 10 min, centrifuged at 4200 r / min for 5 min, took 20 mL of the supernatant, concentrated it to about 1 mL by rotary evaporation in a water bath at 40 °C, and waited for purification.

[0096] 1.3 Use CarbonNH2 column, add about 2cm high anhydrous sodium sulfate to Carbon / NH2 column. Rinse the SPE column with 5mL acetonitrile + toluene (3+1, v / v) first, and discard the eff...

Embodiment 2

[0108] An implementation example of GC-Q-Orbitrap screening and confirmation technology for more than 600 pesticides (such as those described above) in tomato.

[0109] The sample pretreatment steps, GC-Q-Orbitrap operating conditions and the screening process of pesticide residues in the samples all refer to Example 1.

[0110] GC-Q-Orbitrap screening results in tomato samples from a provincial capital city: 16 commercially available tomato samples from a provincial capital city were collected, more than 600 pesticide residues were screened using GC-Q-Orbitrap technology, and GC-Q-Orbitrap technology detected There are 16 kinds of pesticide residues, with a total of 45 frequencies, involving 15 samples. The specific results are shown in Table 4.

[0111] Table 4 GC-Q-Orbitrap screening results in tomato samples from a certain area

[0112]

Embodiment 3

[0114] Implementation example of GC-Q-Orbitrap screening and confirmation technology for more than 600 pesticides in grapes (such as the pesticides described above).

[0115] The sample pretreatment steps, GC-Q-Orbitrap operating conditions and the screening process of pesticide residues in the samples all refer to

[0116] According to Example 1.

[0117] GC-Q-Orbitrap screening results of grape samples in a provincial capital city: Collected 16 commercially available grape samples in a provincial capital city, applied GC-Q-Orbitrap technology to screen more than 600 pesticide residues, and GC-Q-Orbitrap technology detected 21 kinds of pesticide residues, a total of 73 frequencies, involving 16 samples, the specific results are shown in Table 5.

[0118] Table 5 GC-Q-Orbitrap screening results in grape samples in a certain area

[0119]

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Abstract

The invention discloses an electronic identity database and a detection method of pesticide compounds in edible agricultural products based on a GC-Q-Orbitrap. The electronic identity database contains an information collection of electronic identity cards for a plurality of pesticide compounds and is ordered according to the retention time in the electronic identity cards. The electronic identitycard includes pesticide compound information, retention time, mass spectrum, and fragment ion information and a smart matching value. The detection method comprises the steps of sample pretreatment,GC-Q-Orbitrap operating condition setting and pesticide residue screening process in the sample. The GC-Q-Orbitrap operating condition setting comprises the step of setting appropriate chromatographicconditions and mass spectrometry conditions. The pesticide screening process comprises the steps of: first using the retention time to find a pesticide compound in the electronic identity database, and if matched, extracting information of a corresponding electronic identity card; and then comparing the smart matching value, and if less than a threshold, recording and displaying the result to complete the screening.

Description

technical field [0001] The invention designs an electronic identity database and detection method of pesticide compounds in edible agricultural products based on GC-Q-Orbitrap. The invention can realize non-target, multi-indicator and rapid screening for more than 600 pesticide residues in various edible agricultural products. Background technique [0002] As early as 1976, the World Health Organization (WHO), the Food and Agriculture Organization (FAO) and the United Nations Environment Program (UNEP) jointly established the Global Environment Monitoring System / Food Project (Global Environment Monitoring System, GEMS / Food), aiming to master member states The status of food contamination, understanding the intake of food contaminants, protecting human health and promoting trade development. Now, all countries in the world have raised food safety to the strategic position of national security. Pesticide residue limit is one of the food safety standards and also the entry thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8696
Inventor 庞国芳范春林吴兴强韩奎国常巧英张紫娟陈辉白若镔
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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