Preserving fluid for mesenchymal stem cells, preparation method and application thereof

A technology of quality stem cells and preservation solution, which is applied in the field of stem cell preparation preservation solution, can solve the problems of decreased therapeutic effect and low cell activity, and achieve the effect of avoiding rapid decline in cell activity or even death

Inactive Publication Date: 2019-04-05
药鼎(北京)国际细胞医学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Clinically, 0.9% sodium chloride injection, 5% glucose injection, 1% human serum albumin solution and 5% human serum albumin solution, and compound electrolyte injection are commonly used as preparation preservation solutions for umbilical cord mesenchymal stem cells. After 6 hours, the cell activity is very low, which will cause a serious decline in the therapeutic effect

Method used

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  • Preserving fluid for mesenchymal stem cells, preparation method and application thereof
  • Preserving fluid for mesenchymal stem cells, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] This embodiment provides a mesenchymal stem cell preservation solution.

[0045] A mesenchymal stem cell preservation solution, which includes Bomaili A electrolyte injection, insulin, estrogen, progesterone, sodium selenite, heparin calcium, human serum albumin, hydrocortisone, adenosine, acetyl cysteine ​​and glutamine.

[0046] In the mesenchymal stem cell preservation solution, the final concentration of insulin is 0.2 μg / L, and the final concentration of estrogen is 1ⅹ10 - 8 mol / L, the final concentration of progesterone is 1ⅹ10 -9 mol / L, the final concentration of sodium selenite is 30nmol / L, the concentration of heparin calcium is 50IU / mL, the final concentration of human albumin is 1%, and the final concentration of hydrocortisone is 1ⅹ10 -6 mol / L, the final concentration of adenosine is 0.5mmol / L, the final concentration of acetylcysteine ​​is 2mmol / L, and the final concentration of glutamine is 30mmol / L.

Embodiment 2

[0048] This embodiment provides a mesenchymal stem cell preservation solution, which is roughly the same as the embodiment, except that the parameters are different, and the differences are as follows:

[0049] A mesenchymal stem cell preservation solution, which includes Bomaili A electrolyte injection, insulin, estrogen, progesterone, sodium selenite, heparin calcium, human serum albumin, hydrocortisone, adenosine, acetyl cysteine ​​and glutamine.

[0050] In the mesenchymal stem cell preservation solution, the final concentration of insulin was 0.18 μg / L, and the final concentration of estrogen was 1.5ⅹ10 -8 mol / L, the final concentration of progesterone is 1ⅹ10 -9 mol / L, the final concentration of sodium selenite is 30nmol / L, the concentration of heparin calcium is 50IU / mL, the final concentration of human albumin is 1%, and the final concentration of hydrocortisone is 1ⅹ10 -6 mol / L, the final concentration of adenosine is 0.5mmol / L, the final concentration of acetylcyst...

Embodiment 3

[0052] This embodiment provides a mesenchymal stem cell preservation solution, which is roughly the same as Embodiments 1-2, the difference lies in the different parameters, and the differences are as follows:

[0053] A mesenchymal stem cell preservation solution, which includes Bomaili A electrolyte injection, insulin, estrogen, progesterone, sodium selenite, heparin calcium, human serum albumin, hydrocortisone, adenosine, acetyl cysteine ​​and glutamine.

[0054] In the mesenchymal stem cell preservation solution, the final concentration of insulin is 0.22 μg / L, and the final concentration of estrogen is 2ⅹ10 -8 mol / L, the final concentration of progesterone is 1ⅹ10 -9 mol / L, the final concentration of sodium selenite is 28nmol / L, the final concentration of heparin calcium is 50IU / mL, the final concentration of human serum albumin is 1%, and the final concentration of hydrocortisone is 1ⅹ10 -6 mol / L, the final concentration of adenosine is 0.5mmol / L, the final concentrati...

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Abstract

The embodiment of the invention provides a preserving fluid for mesenchymal stem cells, a preparation method and an application thereof. The preserving fluid for mesenchymal stem cells includes multiple electrolytes injection, insulin, estrogen, progestational hormone, sodium selenite, heparin calcium and human serum albumin. The preserving fluid composed of the components is capable of supportinglife state of stem cells, especially umbilical cord mesenchymal stem cells, is capable of preventing cell viability from quickly decreasing and even preventing cells from death, and has the advantageof capability of long-term preserving stem cells.

Description

technical field [0001] The present invention relates to the technical field of stem cell preparation preservation solution, in particular to a mesenchymal stem cell preservation solution, its preparation method and its application. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from mesoderm with high self-renewal ability and multilineage differentiation potential. In fact, MSCs widely exist in various tissues throughout the body, and almost come from all tissues and organs of the body, such as bone marrow, periosteum, adipose tissue, dental pulp, synovium, umbilical cord, placenta, amniotic fluid and fetal tissue. [0003] Mesenchymal stem cells from different sources have similar morphology, express the same surface markers, and have similar biological characteristics. For example, they can be cultured and expanded in vitro, and can differentiate into neurons, osteoblasts, Cells of multiple tissue systems including chondrocy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/08A61P7/06A61P37/02A61P29/00A61P35/00
CPCA01N1/0226A61K9/0019A61K9/08A61K35/28A61P7/06A61P29/00A61P35/00A61P37/02
Inventor 周海涛
Owner 药鼎(北京)国际细胞医学技术有限公司
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