Enzyme catalyst with local photothermal effect and preparation method of enzyme catalyst
A photothermal effect and enzyme catalyst technology, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of inability to promote local heating, poor enzyme applicability, etc., and achieve good catalytic effect, mild conditions, and simple preparation methods Effect
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Embodiment 1
[0060] Preparation of Candida Antarctica Lipase-Enzyme Catalyst for Photothermal Materials
[0061] (1) 10 mg ferroferrogen tetroxide magnetic graphene (purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd., No. XF018, the ferroferromagnetic graphene described below was purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd. company, No. XF018) was dispersed in 20ml, 20mM, Tris-HCl buffer solution with pH=8.0, and ultrasonically treated for 0.5h to obtain mixture A. The scanning electron microscope and transmission electron microscope pictures are shown in figure 1 and figure 2 ;
[0062] (2) Dissolve 1mg of polydopamine in 2ml of deionized water to obtain a polydopamine solution, inject the polydopamine solution into mixture A, shake and mix at 50°C for 0.01h, and absorb it with a magnet to obtain solid B, wash solid B with deionized water 3 Then, B was dispersed in 10ml deionized water to obtain mixture C. The scanning electron microscope and trans...
Embodiment 2
[0065] Preparation of horseradish peroxidase-enzyme catalyst for photothermal materials
[0066] (1) Disperse 1mg of graphene oxide in 0.2ml, 20mM, Tris-HCl buffer solution with pH=7.0, and sonicate for 1h to obtain mixture A;
[0067] (2) Dissolve 1 mg of polydopamine in 0.2 ml of deionized water to obtain a polydopamine solution, inject the polydopamine solution into mixture A, shake and mix for 1 hour at 4°C, and absorb it with a magnet to obtain solid B, wash solid B with deionized water 5 times, then B was dispersed in 100ml deionized water to obtain mixture C;
[0068] (3) 0.22mg horseradish peroxidase was dissolved in 0.11ml, 50mM, in the PB buffer solution of pH=7.0, centrifuged at 9000r / min for 10min, filtered through a membrane to obtain horseradish peroxidase solution, Mix the horseradish peroxidase solution with C, shake and mix at 50°C for 0.01 h, and then adsorb with a magnet to obtain solid D, which is washed with deionized water three times.
Embodiment 3
[0070] Preparation of organophosphate hydrolase-enzyme catalyst for photothermal materials
[0071] (1) Disperse 22mg of graphene oxide in 30ml, 20mM, Tris-HCl buffer solution with pH=9.0, and sonicate for 1h to obtain mixture A;
[0072] (2) Dissolve 100 mg of polydopamine in 100 ml of deionized water to obtain a polydopamine solution, inject the polydopamine solution into mixture A, shake and mix for 2 hours at 25°C, and absorb it with a magnet to obtain solid B, wash solid B with deionized water 4 times , and then disperse B in 50ml deionized water to obtain mixture C;
[0073] (3) Dissolve 0.00022 mg of organophosphorus hydrolase in 0.00055 ml, 50 mM, PB buffer solution with pH=9.0, centrifuge at 10,000 r / min for 10 min, filter through a membrane to obtain organophosphorus hydrolase solution, and hydrolyze the organophosphorus The enzyme solution was mixed with C, shaken and mixed for 2 hours at 20°C, and then adsorbed by a magnet to obtain solid D, which was washed with ...
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