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Application of soybean ger protein coding gene gmger12

A technology that encodes genes and proteins, applied in the field of genetic engineering, can solve the problem that the effective phosphorus concentration is difficult to meet the needs of plant growth, and achieve the effect of improving low phosphorus tolerance and easy absorption

Active Publication Date: 2021-06-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in many agricultural ecosystems, the available phosphorus concentration in the soil is difficult to meet the needs of plant growth

Method used

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  • Application of soybean ger protein coding gene gmger12
  • Application of soybean ger protein coding gene gmger12
  • Application of soybean ger protein coding gene gmger12

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Experimental program
Comparison scheme
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Embodiment 2

[0035] Genetic engineering application of embodiment 2 gene GmGER12

[0036] 1) Cloning of soybean GER protein coding gene GmGER12

[0037] Using the root total RNA of soybean (Glycine max) low-phosphorus-resistant material CD as a template, the first strand of cDNA was synthesized by reverse transcription, and PCR amplification was carried out. The primer sequences are shown in SEQ ID NO.3 and SEQ ID NO.4, PCR The program is as follows: pre-denaturation at 95°C for 3 minutes, denaturation at 95°C for 15 seconds, annealing at 60°C for 15 seconds, extension at 72°C for 1 minute, a total of 35 cycles, and finally incubation at 72°C for 5 minutes, followed by constant temperature at 12°C, and the PCR product was cloned into pAN580 vector, the CDS sequence of the 669bp soybean GmGER12 gene with a complete coding region was obtained after sequencing, wherein the sequence of the coding region is shown in SEQ ID NO.1;

[0038] 2) Construction of plant expression vectors

[0039] Th...

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Abstract

The invention discloses the application of soybean GER protein coding gene GmGER12. The soybean GER protein coding gene GmGER12 has a nucleotide sequence of SEQ ID NO.1. The constructed plant overexpression vector pMDC83‑GmGER12 was heterologously expressed in the wild type of Arabidopsis thaliana, and it was found that the length and number of root hairs in the overexpressed plants would increase, indicating that the gene could be introduced into plants as a target gene. The change makes it easier to absorb phosphorus, thereby improving the ability of transgenic plants to tolerate low phosphorus. It can be seen that the soybean GER protein encoding gene GmGER12 of the present invention can be used in increasing the number and length of root system and improving the ability of transgenic plants to tolerate low phosphorus through genetic engineering.

Description

technical field [0001] The present invention relates to the application of soybean germin-like (GER) protein coding gene GmGER12, which belongs to the field of genetic engineering, and specifically relates to the increase of the length and number of plant root hairs by the germin protein GmGER12 gene derived from soybean, thereby affecting the low tolerance of transgenic plants. Application of phosphorus capacity. Background technique [0002] As one of the essential mineral elements for plant growth and development, phosphorus element participates in the processes of photosynthesis, respiration, carbon metabolism and energy transfer in plants. However, in many agro-ecosystems, the available phosphorus concentration in the soil is difficult to meet the needs of plant growth. Therefore, studying the functions of soybean phosphorus efficiency-related genes and further analyzing the molecular regulation mechanism of phosphorus elements have important theoretical and production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/06A01H6/20
CPCC07K14/415C12N15/8261C12N15/8271
Inventor 程浩王晴杜文凯杨宇明赵梦刘永顺喻德跃
Owner NANJING AGRICULTURAL UNIVERSITY