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[Alpha]1-microglobulin detection kit

A detection kit and microglobulin technology, applied in the biological field, can solve the problems of high price, increased equipment cost, and low analysis speed, and achieve the effects of reduced inspection costs, low cost, and large throughput

Active Publication Date: 2019-04-05
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because the determination of α1-MG is relatively difficult, if no pretreatment or other special methods are used, the determination of α1-MG will be underestimated to varying degrees. Low, poor correlation with clinical samples
The α1-MG assay reagent currently on the market as the gold standard is the α1-MG reagent on the Siemens specific protein analysis system BNII; and the Siemens α1-MG reagent is used on its specific protein analysis system BNII, and the analysis speed is lower than 250test / hour, and the price is expensive; it cannot meet the needs of large hospitals and medical examination centers for massive sample testing, as well as the country's need to reduce examination costs
Most inspection centers use the method of purchasing multiple Siemens BNII systems to increase throughput and further increase equipment costs

Method used

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Examples

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Effect test

Embodiment 1

[0053] This embodiment provides a α1-microglobulin detection kit, the kit includes a reagent R1 and a reagent R2, and the reagent R2 is a latex microsphere solution labeled with an α1-MG antibody;

[0054] Reagent R1: citric acid-NaOH buffer solution with a concentration of 50mM / L, pH5.5; TCEP 20g / L; sodium chloride 600mM / L; Tween-205ml / L; polyethylene glycol 60002g / L; preservative PC- 3000.5ml / L;

[0055] Reagent R2: 10mM phosphate buffer with pH7.8; Tween-202ml / L; preservative PC-3000.5ml / L; 100nm latex microspheres 3.6g / L; Dako product number Q0495 α1-MG antibody 20ml / L;

[0056] Using common α1-MG reagent parameters, 2ul sample, 200ul reagent R1, 67ul reagent R2, 570nm single wavelength, tested on Hitachi 7180 automatic biochemical analyzer, ΔABS (absorbance change value) was calculated using the two-point endpoint method, 18- 34 photometric points are read, and it can be calculated that the Nacl concentration in the final reaction solution buffer after mixing the final r...

Embodiment 2

[0072] This embodiment provides a α1-microglobulin detection kit, the kit includes a reagent R1 and a reagent R2, and the reagent R2 is a latex microsphere solution labeled with an α1-MG antibody;

[0073] Reagent R1: citric acid-NaOH buffer solution with a concentration of 50mM / L, pH6.3; TCEP 20g / L; sodium chloride 1400mM / L; Tween-205ml / L; polyethylene glycol 60002g / L; preservative PC- 3000.5ml / L;

[0074] Reagent R2: 10mM phosphate buffer at pH6.9; Tween-202ml / L; preservative PC-3000.5ml / L; 100nm latex microspheres 3.6g / L; Dako product number Q0495 α1-MG antibody 20ml / L;

[0075] Using common α1-MG reagent parameters, 2ul sample, 200ul reagent R1, 67ul reagent R2, 570nm single wavelength, tested on Hitachi 7180 automatic biochemical analyzer, ΔABS (absorbance change value) was calculated using the two-point endpoint method, 18- 34 photometric points are read, and it can be calculated that the Nacl concentration in the final reaction solution buffer after mixing the final re...

Embodiment 3

[0091] This embodiment provides a α1-microglobulin detection kit, the kit includes a reagent R1 and a reagent R2, and the reagent R2 is a latex microsphere solution labeled with an α1-MG antibody;

[0092]Reagent R1: citric acid-NaOH buffer solution with a concentration of 100mM / L, pH6.3; TCEP 50g / L; sodium chloride 1400mM / L; Tween-205ml / L; polyethylene glycol 60002g / L; preservative PC- 3000.5ml / L;

[0093] Reagent R2: 10mM phosphate buffer at pH6.9; Tween-202ml / L; preservative PC-3000.5ml / L; 100nm latex microspheres 3.6g / L; Dako product number Q0495 α1-MG antibody 20ml / L;

[0094] Using common α1-MG reagent parameters, 2ul sample, 200ul reagent R1, 67ul reagent R2, 570nm single wavelength, tested on Hitachi 7180 automatic biochemical analyzer, ΔABS (absorbance change value) was calculated using the two-point endpoint method, 18- 34 photometric point readings, it can be calculated that the concentration of Nacl in the final reaction solution buffer after mixing the final reag...

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Abstract

The invention discloses a [Alpha]1-microglobulin detection kit comprising a reagent R1 and a reagent R2; the reagent R2 is latex microsphere solution marked with [Alpha]1-MG antibodies; the reagent R1comprises 50-100mM / L citric acid-NaOH buffer solution, 20-50g / L TCEP and 600-1400mM / L sodium chloride; the reagent R2 comprises 10mM phosphate buffer solution, 3.6g / L 80-120nm latex microspheres and20ml / L [Alpha]1-MG antibodies. The invention provides the [Alpha]1-microglobulin detection kit, so that the [Alpha]1-microglobulins in urine and serum samples are accurately measured without affectingthe sensitivity; in addition, the kit provided by the invention is relatively low in cost, and accords with the national requirement of reducing the inspection charge.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an α1-microglobulin detection kit. Background technique [0002] α1-MG is widely present in various body fluids and the surface of lymphocyte membranes in the human body. α1-MG exists in two forms in the blood, namely free α1-MG and α1-MG bound to IgA (α1MG-IgA). Under normal circumstances, α1MG-IgA accounts for about 40-70% of the total α1-MG in the blood, and the level of immunoglobulin in the blood has an impact on the ratio between α1-MG and α1MG-IgA. Free α1-MG in the blood can freely pass through the glomerular filtration membrane, 95%-99% is reabsorbed and metabolized in the renal proximal tubule, and only a small amount is excreted in the final urine; while α1MG-IgA cannot pass through the normal renal tubule ball, its concentration in normal human urine is zero. The epitope of α1-MG and IgA binding surface (The epitope of α1-MG and IgA bingding site hereinafter abbreviated...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/543G01N35/00
CPCG01N33/5306G01N33/54393G01N35/00
Inventor 王钊
Owner BYRON DIAGNOSTICS SHANGHAI
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