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Preparation method of scallop protein isolate-beta-carotene emulsion

A technology for separating protein and carotene, which is applied in the field of food processing, can solve the problems of a large amount of food industry waste and high yield of scallops, and achieve the effects of delicate taste, high embedding rate and improved utilization rate

Inactive Publication Date: 2019-04-12
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the production of scallops is high, it also produces a large amount of food industry waste

Method used

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  • Preparation method of scallop protein isolate-beta-carotene emulsion
  • Preparation method of scallop protein isolate-beta-carotene emulsion
  • Preparation method of scallop protein isolate-beta-carotene emulsion

Examples

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Effect test

Embodiment 1

[0066] A preparation method of scallop protein isolate-beta-carotene emulsion, through the following process steps:

[0067] S1. Prepare scallop gonad degreasing powder: Homogenize scallop female gonad at 2000pm for 5 minutes, add 10 times the volume of n-hexane / absolute ethanol (3:1, v:v) mixture, stir at 4°C for 6h for degreasing, pumping Filter, air-dry the filter cake naturally, pulverize it with a ball mill, and pass through a 100-mesh sieve to obtain a defatted powder.

[0068] S2. Preparation of scallop protein isolate: mix the defatted scallop female gonad powder with water at a ratio of 1:5 (g / mL), adjust the pH to 11.5 with 1M NaOH, stir at room temperature for 2 hours, and centrifuge at 2000rpm for 15 minutes to take the supernatant Use 1M HCl solution to adjust the pH to 3.8, let it stand at room temperature for 2 hours, centrifuge at 9000rpm for 30 minutes, dissolve the precipitate in deionized water, stir well, adjust the pH to 7, use 2KDa dialysis membrane, 10mM...

Embodiment 2

[0075] A preparation method of scallop protein isolate-beta-carotene emulsion, through the following process steps:

[0076] S1. Prepare scallop gonad degreasing powder: Homogenize scallop female gonad at 2500pm for 4min, add 10 times the volume of n-hexane / absolute ethanol (2:1, v:v) mixture, stir at 4°C for 7h for degreasing, pumping Filter, air-dry the filter cake naturally, pulverize it with a ball mill, and pass through a 100-mesh sieve to obtain a defatted powder.

[0077] S2. Preparation of scallop protein isolate: Mix scallop female gonad degreased powder and water at a ratio of 1:20 (g / mL), adjust the pH to 11.5 with 1M NaOH, stir at room temperature for 2 hours, and centrifuge at 2000rpm for 15 minutes to take Adjust the pH to 3.8 with 1M HCl solution, let stand at room temperature for 2 hours, centrifuge at 10,000rpm for 20 minutes, dissolve the precipitate in deionized water, stir well, adjust the pH to 7, use a 3KDa dialysis membrane, 10mM pH7.4 phosphate Dialyze...

Embodiment 3

[0084] A preparation method of scallop protein isolate-beta-carotene emulsion, through the following process steps:

[0085] S1. Prepare scallop gonad degreasing powder: Homogenize scallop female gonad at 3000pm for 3min, add 10 times the volume of n-hexane / absolute ethanol (3:1, v:v) mixture, stir at 4°C for 8h for degreasing, pumping Filter, air-dry the filter cake naturally, pulverize it with a ball mill, and pass through a 100-mesh sieve to obtain a defatted powder.

[0086] S2. Preparation of scallop protein isolate: mix scallop female gonad defatted powder with water at a ratio of 1:10 (g / mL) to liquid, adjust pH to 11.5 with 1M NaOH, stir at room temperature for 2 hours, and centrifuge at 2000 rpm for 15 minutes Take the supernatant, adjust pH3.8 with 1M HCl solution, let stand at room temperature for 2h, centrifuge at 12000rpm for 15min, dissolve the precipitate in deionized water, stir well, adjust the pH value to 7, use 2KDa dialysis membrane, 10mM pH7.4 Phosphate b...

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Abstract

The invention discloses a preparation method of a scallop protein isolate-beta-carotene emulsion. The method comprises the following steps: preparing scallop gonad degreased powder, preparing scallopprotein isolate, preparing a scallop protein isolate solution, preparing an oil phase, preparing a primary emulsion and preparing the nutrient-loading emulsion. The method uses scallop female gonad protein isolate as an emulsifier and edible oil as a carrier oil phase, adopts a dynamic high pressure micro-jet technology to prepare the emulsion, and embeds beat-carotene to prepare the novel and stable emulsion capable of effectively delivering nutrients. The emulsion prepared by the method has lower cost, can improve the stability of the beta-carotene, and has a good application prospect in thefood industry. The invention not only provides a new method for preparing the beta-carotene emulsion, but also further expands the application of the bata-carotene and scallop female gonads in the food field.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method of scallop protein isolate beta-carotene emulsion. Background technique [0002] As a typical fat-soluble bioactive substance, β-carotene is the most widely distributed carotenoid in nature and the most studied by scholars. As a precursor of vitamin A, it is widely used as an antioxidant and natural colorant, and plays a vital role in the prevention of many diseases in the human body (such as cancer, cardiovascular disease, macular degeneration, cataracts). However, due to the polyunsaturated structure of β-carotene, it is usually accelerated by light, heat, enzymes, metal ions, metal-binding proteins, and microorganisms, so its chemical instability and low bioavailability And low water solubility greatly limits its application in various aqueous solutions. Therefore, the use of oil-in-water emulsions to deliver β-carotene can be a good way to solve...

Claims

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Application Information

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IPC IPC(8): A23L33/10A23L33/17A23J1/04
CPCA23J1/04A23L33/10A23L33/17
Inventor 吴海涛韩佳润阎佳楠杜椅楠商文慧洪佳男
Owner DALIAN POLYTECHNIC UNIVERSITY
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