Preparation method of scallop protein isolate-beta-carotene emulsion

A technology for separating protein and carotene, which is applied in the field of food processing, can solve the problems of a large amount of food industry waste and high yield of scallops, and achieve the effects of delicate taste, high embedding rate and improved utilization rate

Inactive Publication Date: 2019-04-12
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, while the production of scallops is high, i

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  • Preparation method of scallop protein isolate-beta-carotene emulsion
  • Preparation method of scallop protein isolate-beta-carotene emulsion
  • Preparation method of scallop protein isolate-beta-carotene emulsion

Examples

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[0065] Example 1

[0066] A kind of preparation method of scallop protein isolate-beta-carotene emulsion, goes through the following process steps:

[0067] S1. Preparation of scallop gonad degreasing powder: Homogenize scallop female gonads at 2000 pm for 5 minutes, add 10 times the volume of n-hexane / absolute ethanol (3:1, v:v) mixture, stir at 4°C for 6 hours to degrease, and extract Filter, air-dry the filter cake, pulverize with a ball mill, pass through a 100-mesh sieve, and obtain a defatted powder.

[0068] S2. Preparation of scallop protein isolate: Mix the scallop female gonad defatted powder with water at a ratio of 1:5 (g / mL) to liquid, adjust the pH to 11.5 with 1M NaOH, stir at room temperature for 2 hours, and centrifuge at 2000 rpm for 15 minutes to take the supernatant Adjust pH3.8 with 1M HCl solution, stand at room temperature for 2h, centrifuge at 9000rpm for 30min, dissolve the precipitate in deionized water, stir well, adjust pH to 7, use 2KDa dialysis m...

Example Embodiment

[0074] Example 2

[0075] A kind of preparation method of scallop protein isolate-beta-carotene emulsion, goes through the following process steps:

[0076] S1. Preparation of scallop gonad degreasing powder: Homogenize scallop female gonads at 2500 pm for 4 min, add 10 times the volume of n-hexane / absolute ethanol (2:1, v:v) mixture, stir at 4°C for 7 hours to degrease, and extract Filter, air-dry the filter cake, pulverize with a ball mill, pass through a 100-mesh sieve, and obtain a defatted powder.

[0077] S2. Preparation of scallop protein isolate: Mix scallop female gonad degreasing powder with water at a ratio of 1:20 (g / mL) to the ratio of material to liquid, adjust the pH to 11.5 with 1M NaOH, stir at room temperature for 2 hours, and centrifuge at 2000 rpm for 15 minutes. The clear was adjusted to pH 3.8 with 1M HCl solution, left standing at room temperature for 2 h, centrifuged at 10,000 rpm for 20 min, the precipitate was dissolved in deionized water, stirred we...

Example Embodiment

[0083] Example 3

[0084] A kind of preparation method of scallop protein isolate-beta-carotene emulsion, goes through the following process steps:

[0085] S1. Preparation of scallop gonad degreasing powder: Homogenize scallop female gonads at 3000 pm for 3 min, add 10 times the volume of n-hexane / absolute ethanol (3:1, v:v) mixture, stir at 4°C for 8 hours to degrease, and extract Filter, air-dry the filter cake, pulverize with a ball mill, pass through a 100-mesh sieve, and obtain a defatted powder.

[0086] S2. Preparation of scallop protein isolate: Mix scallop female gonad defatted powder and water at a ratio of 1:10 (g / mL) to the ratio of material to liquid, use 1M NaOH to adjust pH11.5, stir at room temperature for 2h, and centrifuge at 2000rpm for 15min Take the supernatant, adjust pH3.8 with 1M HCl solution, stand at room temperature for 2h, centrifuge at 12000rpm for 15min, dissolve the precipitate in deionized water, stir evenly, adjust the pH value to 7, use 2KDa...

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Abstract

The invention discloses a preparation method of a scallop protein isolate-beta-carotene emulsion. The method comprises the following steps: preparing scallop gonad degreased powder, preparing scallopprotein isolate, preparing a scallop protein isolate solution, preparing an oil phase, preparing a primary emulsion and preparing the nutrient-loading emulsion. The method uses scallop female gonad protein isolate as an emulsifier and edible oil as a carrier oil phase, adopts a dynamic high pressure micro-jet technology to prepare the emulsion, and embeds beat-carotene to prepare the novel and stable emulsion capable of effectively delivering nutrients. The emulsion prepared by the method has lower cost, can improve the stability of the beta-carotene, and has a good application prospect in thefood industry. The invention not only provides a new method for preparing the beta-carotene emulsion, but also further expands the application of the bata-carotene and scallop female gonads in the food field.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method of scallop protein isolate beta-carotene emulsion. Background technique [0002] As a typical fat-soluble bioactive substance, β-carotene is the most widely distributed carotenoid in nature and the most studied by scholars. As a precursor of vitamin A, it is widely used as an antioxidant and natural colorant, and plays a vital role in the prevention of many diseases in the human body (such as cancer, cardiovascular disease, macular degeneration, cataracts). However, due to the polyunsaturated structure of β-carotene, it is usually accelerated by light, heat, enzymes, metal ions, metal-binding proteins, and microorganisms, so its chemical instability and low bioavailability And low water solubility greatly limits its application in various aqueous solutions. Therefore, the use of oil-in-water emulsions to deliver β-carotene can be a good way to solve...

Claims

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Application Information

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IPC IPC(8): A23L33/10A23L33/17A23J1/04
CPCA23J1/04A23L33/10A23L33/17
Inventor 吴海涛韩佳润阎佳楠杜椅楠商文慧洪佳男
Owner DALIAN POLYTECHNIC UNIVERSITY
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