Application of Pallidum intermedia in Degradation of Triadimefon
A technology of Pallidum intermedium and triadimefon, which is applied in the application field of Pallidum intermedia in degrading triadimefon, can solve the problems that the research on triadimefon is still blank, and achieve good application prospects and safe and reliable results
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Embodiment 1
[0044] (1) Enrichment and domestication of pesticide-degrading bacteria
[0045] Take fresh Daqu from Fenjiu Factory as the source of screening strains, weigh 10g of Daqu in a 90ml saline flask, incubate at 30°C and 180rpm for 40min, then take 1ml and transfer it to 9ml MSM medium containing 50mg / kg, at 30 Cultivate at 180rpm for 7 days, transfer 1ml into 9ml MSM medium containing 100mg / kg, culture at 30℃, 180rpm for 7d, continue to transfer according to the above operations, and gradually increase the concentration of pesticides to 200-400mg / kg .
[0046] (2) Isolation and purification of pesticide-degrading bacteria
[0047] The enrichment and acclimatization experiment of degrading bacteria lasted 28 days in the early stage, and in the later stage, the bacteria that were cultured for 28 days at the concentration of 400mg / kg pesticide transferred for the fourth time were subjected to stock solution, 10 -1 、10 -2 、10 -3 Spread on MSM solid medium containing 100 mg / kg pest...
Embodiment 2
[0057] The bacterial liquid after resuspending each strain was added to the MSM liquid medium containing different concentrations of pesticides according to the addition amount of 5% and glucose according to the addition amount of 1%. , 10 times the concentration. The total system is 10ml. Cultivate on a shaking table (150rpm) at 30°C for 7 days as a treatment group. After 7 days, the degradation rates of pesticides by various strains in MSM medium with 2, 5 and 10 times the pesticide concentration were measured. In the control test, no bacterial suspension was added, and the change of the pesticide concentration was measured after being placed under the same conditions for 7 days. The control group and the treatment group were respectively made 3 parallels.
[0058] Take a sample of 3ml every day, of which 1ml is measured by a UV spectrophotometer to characterize the growth of the bacterial strain OD 600nm Value, another 2ml is measured triadimefon concentration with gas ch...
Embodiment 3
[0066] The expansion medium contains 1.5g / L NH 4 NO 3 , 0.5g / L KH 2 PO 4 , 1.5g / L K 2 HPO 4 , 1.0g / L NaCl, 0.2g / L MgSO 4 ·7H 2 O's MSM medium. Ready to use after sterilization.
[0067] Triadimefon was purchased from Bailingwei J&K Company with a purity of 98.5%.
[0068] Pick a single colony of Paleobacterium intermedium F1 from the LB solid medium plate of the preserved strain and inoculate it into a vial of LB broth medium, and cultivate it in a shaker at 30°C and 150rpm until the OD of the bacterial solution 600 =0.8, centrifuge the bacterial solution at 5000×g for 5min, discard the supernatant, wash the bacterial precipitate with MSM liquid medium, mix well, repeat washing twice, add MSM liquid medium, and oscillate with a vortex shaker Resuspend and mix the bacteria thoroughly to prepare a degrading bacteria seed solution, in which the number of viable bacteria of Pallidinium intermedia F1 is 80×10 8 CFU / ml.
[0069] Set up 3 groups of experiments as shown in ...
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