Method for preparing low molecular chitosan by gradient ultrasonic

A low-molecular-weight chitosan and gradient ultrasound technology, applied in the field of low-molecular-weight chitosan preparation, can solve the problems of unsuitable control of hydrolysis degree, wide product molecular weight distribution, long enzymatic production cycle, etc., and achieves safe and reliable degradation process, molecular weight The effect of narrow distribution and simple post-processing

Inactive Publication Date: 2019-04-16
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the chemical method has significant defects. The production conditions are relatively harsh, often involving high temperature and high pressure. It is especially important that the degree of hydrolysis is not suitable for control, the molecular weight distribution of the product is wide, and a large amount of chemical reagents are used in the preparation process, which is harmful to the environment.
Although the enzymatic method can be carried out under relatively mild conditions and has high specificity, the production cycle of the enzymatic method is long, and there are still some problems in selecting high-activity enzymes and low-cost separation and purification methods to be suitable for industrialized large-scale production. difficulty

Method used

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  • Method for preparing low molecular chitosan by gradient ultrasonic
  • Method for preparing low molecular chitosan by gradient ultrasonic
  • Method for preparing low molecular chitosan by gradient ultrasonic

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Effect test

Embodiment 1

[0019] A kind of method of the present invention prepares low-molecular chitosan with gradient ultrasonic, comprises the following steps:

[0020] (1) Dissolution of high molecular weight chitosan: dissolving high molecular weight chitosan with a molecular weight of 10kDa to 200kDa in an acetic acid solution with a volume fraction of 0.1% to 10% to obtain a chitosan solution with a volume fraction of 0.5% to 30% ;

[0021] Preferably, the molecular weight of high molecular weight chitosan is 50kDa, the volume fraction of acetic acid is 1%, and the volume fraction of chitosan solution is 16%;

[0022] (2) Degradation of high-molecular-weight chitosan: the chitosan solution obtained in step (1) is subjected to ultrasonic treatment three times, and the first ultrasonic treatment sets the ultrasonic temperature to 5~30° C. The duration is 2-6 hours, and the ultrasonic power is 150-300W; the ultrasonic temperature is set at 10-50°C for the second ultrasonic treatment, and the time...

Embodiment 2

[0028] A kind of method of the present invention prepares low-molecular chitosan with gradient ultrasonic, comprises the following steps:

[0029] (1) dissolving of high molecular weight chitosan: the high molecular weight chitosan that molecular weight is 100kDa is dissolved in the acetic acid solution that volume fraction is 5% is that volume fraction is 20% chitosan solution;

[0030] (2) Degradation of high-molecular-weight chitosan: the chitosan solution obtained in step (1) is subjected to three ultrasonic treatments, the first ultrasonic treatment sets the ultrasonic temperature to 20°C, and the ultrasonic power is 200W. 30 minutes, stop for 5 minutes, repeat this way, and the time from the beginning to the end of the first ultrasound is 2 hours. For the second ultrasonic treatment, the ultrasonic temperature was set at 40°C, and the ultrasonic power was 80W. During the ultrasonic treatment, every 30 minutes of ultrasonic treatment was followed by a 5-minute pause, and ...

Embodiment 3

[0034] A method for preparing low-molecular chitosan with gradient ultrasound, comprising the following steps:

[0035] (1) dissolving of high molecular weight chitosan: the high molecular weight chitosan that molecular weight is 200kDa is dissolved in the acetic acid solution that volume fraction is 1% is that volume fraction is 16% chitosan solution;

[0036](2) Degradation of high-molecular-weight chitosan: the chitosan solution obtained in step (1) is subjected to three ultrasonic treatments, the first ultrasonic treatment sets the ultrasonic temperature to 15°C, and the ultrasonic power is 200W. 30 minutes, stop for 5 minutes, repeat this way, the time from the beginning to the end of the first ultrasound is 6 hours. For the second ultrasonic treatment, the ultrasonic temperature was set at 20°C and the ultrasonic power was 80W. During the ultrasonic treatment, every 30 minutes of ultrasonic treatment was followed by a 5-minute pause, and this was repeated. The time from ...

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Abstract

The invention belongs to the technical field of polymer chitosan degradation, and discloses a method for preparing low molecular chitosan by gradient ultrasonic. The method comprises the steps of dissolution of high molecular weight chitosan, degradation of the high molecular weight chitosan, purification of the low molecular weight chitosan and the like, the high molecular weight chitosan is degraded by a gradient ultrasonic method, the above method can effectively shorten a degradation reaction time, an obtained product has narrow molecular weight distribution and a low molecular weight, andthe method is simple and practical, and is suitable for industrial large scale production. In addition, the low molecular weight chitosan obtained by the above method has a good antibacterial ability.

Description

technical field [0001] The invention belongs to the field of low-molecular-weight chitosan preparation, in particular to a method for preparing low-molecular-weight chitosan by gradient ultrasound. Background technique [0002] In people's daily life, bacteria are pathogens that cause serious human diseases, and the development of functional materials with high antibacterial activity is one of the effective methods to prevent bacterial infections. Chitosan is a natural antibacterial material that widely exists in nature and has good biocompatibility and broad-spectrum antibacterial properties. High molecular weight chitosan has poor water solubility, which is not conducive to the exertion of antibacterial properties. In contrast, low molecular weight chitosan made by chemically or physically degrading chitosan has good water solubility, can affect the activity of bacteria by adsorbing the negatively charged cytoplasm of bacteria, and can also be adsorbed on the cell membran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/003
Inventor 乔渊森魏璄璇王立强
Owner HUAQIAO UNIVERSITY
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