Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Avian infectious bronchitis virus YT strain and application thereof

A chicken infectious and bronchitis technology, applied in the field of animal virology, can solve the problems of easy mutation of pathogens, poor cross-protection effect, immunity to chicken flock infection, etc., and achieve good protection effect

Active Publication Date: 2019-04-16
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, "H" series vaccines are still the main vaccines for the prevention and control of IB in China, but the extensive use of vaccines has led to varying degrees of immune evasion, gene mutation, virus recombination, and immune tolerance of IBV, which have led to the clinical manifestations of the disease. constant new changes
Because the IBV pathogen is easily mutated, there are multiple serotypes, and new serotypes and mutant strains are constantly emerging, and the cross-protection effect between different serotypes is poor. If the cross-protection between the vaccine strain used and the circulating strain is poor, IBV infection still occurs in vaccinated flocks

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Avian infectious bronchitis virus YT strain and application thereof
  • Avian infectious bronchitis virus YT strain and application thereof
  • Avian infectious bronchitis virus YT strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A strain of infectious bronchitis virus, the isolation and acquisition process of the virus is as follows:

[0025] In 2012, a broiler flock in a chicken farm in Shandong had clinical respiratory symptoms, and the mortality rate was not high. The dead chickens had swollen kidneys and urate deposition, which was suspected to be infectious bronchitis (IB) infection. Tissues such as trachea and kidney of dead chickens were collected, homogenized according to conventional methods, and after centrifugation, the supernatant was taken to inoculate 10-day-old SPF chicken embryos, and continued incubation for 6 days to observe the lesions of chicken embryos. In the first generation, it was found that lesions such as chicken embryo edema, dysplasia, white urate precipitation in ureter, and HA test in allantoic fluid were negative. Passage the above-mentioned isolated poison, and the typical IB chicken embryo lesions appear in the third generation: the chicken embryo has poor acti...

Embodiment 2

[0027] Relevant detection and analysis of embodiment 2 virus

[0028] 1. Detection of virus content

[0029] The E3 generation virus was diluted 10-fold serially with sterilized physiological saline, and 10 -5 、10 -6 、10 -7 、10 -8 Inoculate 5 10-day-old SPF chicken embryos in each allantoic cavity of 4 dilutions, 0.1ml per embryo, continue to incubate at 36°C-37°C, discard the dead chicken embryos before 24 hours, and inoculate the chicken embryos at 24-37°C. Specific lesions such as dehydration, curling up, and small development of embryo bodies that died or survived within 144 hours were judged as infection. Calculate EID 50 , virus content per 0.1ml ≥ 106.0EID 50 .

[0030] Table 1 EID of E3 generation infectious bronchitis virus 50 The measurement results

[0031]

[0032] Note: The numerator is the number of chicken embryo lesions, and the denominator is the number of experimental chicken embryos.

[0033] The results showed that the average embryo weight of...

Embodiment 3

[0055] The preparation of embodiment 3 inactivated vaccines

[0056] Take the preserved strain, that is, the E3 generation YT strain obtained above, dilute it 100 times with sterilized normal saline, inoculate 10-11 day-old susceptible chicken embryos in the allantoic cavity, 0.2ml per embryo, and seal the pinhole after inoculation. Incubate at 36-37°C without turning the eggs; eggs are illuminated once every 24 hours after inoculation of chicken embryos, and dead embryos are discarded. After that, eggs are illuminated once every 6 hours. Dead chicken embryos are taken out at any time and incubated until 48 hours. Whether or not, take them all out, put the air chamber upright, and cool at 2-8°C for 12-24 hours; keep 10-20 chicken embryos, incubate them for 144 hours, observe the lesion rate of the chicken embryos, and find that more than 90% of the eggs have lesions, which proves the vaccination success.

[0057] Take out the cooled chicken embryo, first use iodine and then 7...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of animal virology, and provides an avian infectious bronchitis virus YT strain and application thereof. The avian infectious bronchitis virus YT strain is named asIBV-YT, the preservation number of the IBV-YT is CGMCC No.16386, a separation and identification process of the strain is provided, it is determined that the IBV-YT belongs to current dominant strainQX type through S1 genetic evolution analysis, verification is achieved through a serum neutralization test, the avian infectious bronchitis virus YT strain as a vaccine strain can cross-react with various existing Chinese serotype strains, and better protection is provided for the poultry industry in China.

Description

technical field [0001] The invention relates to the field of animal virology and provides a chicken infectious bronchitis virus strain and application thereof. Background technique [0002] Infectious bronchitis (IB) is an acute, highly contagious respiratory infectious disease of chickens caused by infectious bronchitis virus (IBV). Typical clinical symptoms are coughing, sneezing, dyspnea, and tracheal rales in sick chickens; decreased egg production and egg quality in laying hens; enlarged and pale kidneys in sick chickens, with a large amount of urate deposition in the form of "mottled kidneys", etc. . Infected chickens can also die due to severe respiratory symptoms and kidney lesions. The disease is highly contagious and affects chickens of different ages in different degrees. When the disease occurs in the chick stage, it will also cause permanent damage to the reproductive system of the hen and seriously affect the later egg production. Chickens of all ages are su...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/215A61P31/14C12R1/93
CPCA61K39/12A61K2039/5252A61K2039/552A61P31/14C12N7/00C12N2770/20021C12N2770/20034
Inventor 徐怀英秦卓明王友令袁小远张玉霞杨金兴孟凯高月花
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com