Application of molecular marker MtD4 in identifying carrot petal male sterility

Abstract

The invention discloses application of a molecular marker MtD4 in identifying carrot petal male sterility. Genomic DNA of a carrot to be tested is used as a template, and a primer pair consisting of aprimer F and a primer R is used for PCR amplification to obtain a DNA fragment. The DNA fragment is the molecular marker MtD4 related to the carrot petal male sterility. The nucleotide sequences of the primer F and the primer R are sequentially shown as the sequence 8 and the sequence 9 in the sequence listing. The molecular marker MtD4 may specifically be a DNA fragment 1 or a DNA fragment 2. The nucleotide sequences of the DNA fragment 1 and the DNA fragment 2 are sequentially shown as the sequence 14 and the sequence 15 in the sequence listing. The carrot variety carrying the DNA fragment1 and not carrying the DNA fragment 2 has the petal male sterility. By using the molecular marker MtD4, the carrot variety with the petal male sterility can be rapidly screened out. The application has important application value.

Description

technical field [0001] The invention belongs to the field of breeding, and in particular relates to the application of the molecular marker MtD4 in identifying carrot petal male sterility. Background technique [0002] Since carrot is a cross-pollinated crop with small flower organs and artificial pollination is difficult, the self-incompatibility of carrot is still unclear, so the use of carrot male sterile lines has become the key to hybrid breeding, without complicated procedures such as artificial pollination and emasculation , the production cost is lower, and the seed purity of the first generation of hybrids can be ensured. [0003] The sources of male sterility of existing carrot cultivars mainly come from wild species, all of which are cytoplasmic male sterility (CMS) (Fang Zhiyuan et al. 2017. Chinese Vegetable Breeding, Beijing: China Agricultural Press. 268-269 .). There are two types of male sterility in carrot: brown anther type and andropetalized type. The ...

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Problems solved by technology

[0004] Nakajima et al. (Nakajima Y., Yamamoto T, Muranaka T and Oeda K, 1999. Genetic variation of petaloid male-sterile cytoplasm of carrot revealed by sequencetagged sites (STSs). Theor Appl Genet 99:837–843.) using RAPD and STS markers Qualitative research was carried out on the mitochondrial DNA of 13 sterile lines and fertile lines, and two specific markers, STS1 and STS4, which can distinguish between sterile lines and fertile lines were obtained. The STS1 marker contains a sequence homologous to the orfB gene. The structure of the STS4 marker is relatively complex, including a retrotransposon-like sequence and a small fragment of chloroplast DNA sequence, but these two markers cannot be used for the identification of petal-flowered sterile lines and maintainer lines of various genotypes

Yu Chunxia et al. (Yu Chunxia, ​​Liang Yi, Li Longnian. 2007. Establishment of the CAPS marker of the petalized carrot male sterility gene atp6. China Agricultural Science Bulletin, 23(12): 68-72.) Development of CAPS marker identification based on the mitochondrial gene atp6 The petalized male sterile line H05A and its maintainer line H05B can produce a 60bp specific fragment in the sterile line, but this marker has not been verified in other materials

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