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Application of biomarker to assessment of oral squamous cell carcinoma hazard levels

A technology of risk level and oral squamous cell carcinoma, which is applied in the field of GSDMA, can solve the problems that the influence of subjective factors cannot be ruled out and the lack of quantitative standards, etc., and achieve the effects of avoiding insufficient treatment intensity, improving treatment effect, and high specificity

Active Publication Date: 2019-04-16
XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no quantitative standard, and the influence of subjective factors cannot be ruled out.

Method used

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  • Application of biomarker to assessment of oral squamous cell carcinoma hazard levels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Screening Gene Markers Related to Oral Squamous Cell Carcinoma

[0077] 1. Sample collection

[0078] 68 oral squamous cell carcinoma tissues and adjacent tissues were collected, including 20 patients with histological grade I (G1), 30 patients with histological grade II (G2), and histological grade III / There were 18 patients with grade IV (G3 / G4). The patient did not receive any treatment before operation. All the above-mentioned samples were obtained with the consent of the organizational ethics committee, and 4 samples were taken from each group for detection and analysis of gene expression profiles, screening of differentially expressed genes, and verification experiments were carried out in all samples of each group.

[0079] 2. Preparation of RNA samples (operated using QIAGEN tissue RNA extraction kit)

[0080] Take out the tissue samples frozen in liquid nitrogen, put the tissue samples into a pre-cooled mortar for grinding, and extract and isolate...

Embodiment 2

[0095] Example 2 QPCR sequencing to verify the differential expression of GSDMA genes

[0096] 1. Large-sample QPCR verification of differential expression of GSDMA genes.

[0097] 2. The RNA extraction steps are as described in Example 1.

[0098] 3. Reverse transcription:

[0099] Use the FastQμant cDNA First Strand Synthesis Kit (Product No.: KR106) for mRNA reverse transcription, first remove the genomic DNA reaction, add 5×gDNA Bμffer 2.0μl, total RNA 1μg, add RNase Free ddH 2O Make the total volume to 10μl, heat in a water bath at 42°C for 3min, then add 2.0μl of 10×Fast RT Bμffer, 1.0μl of RT Enzyme Mix, 2.0μl of FQ-RT Primer Mix, RNase Free ddH 2 O5.0 μl, after mixing, add to the above-mentioned test tube and mix together a total of 20 μl, heat at 42°C for 15 minutes in a water bath, and heat at 95°C for 3 minutes.

[0100] 4. QPCR amplification detection

[0101] QPCR amplification primers were designed according to the sequences of GSDMA and GAPDH, and synthesize...

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Abstract

The invention discloses an application of a biomarker to assessment of oral squamous cell carcinoma hazard levels. The biomarker is GSDMA and is used for detecting oral squamous cell carcinoma sampleswith different differentiation grades to discover that the biomarker presents significant differences in oral squamous cell carcinoma samples with different hazard levels, and prompt to apply the GSDMA to grading judgement of oral squamous cell carcinoma so as to instruct the doctors to adopt different treatment ways for the oral squamous cell carcinoma in different progresses and then improve the treatment effect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of biomarkers in evaluating the risk degree of oral squamous cell carcinoma, and the specifically involved biomarkers are GSDMA. Background technique [0002] Oral Squamous Cell Carcinoma (OSCC) is one of the most malignant tumors, and it is also the most common type of oral tumor. Patients with OSCC are often accompanied by metastasis, high recurrence rate and poor prognosis . Epidemiological statistical analysis About 100,000 people die from oral squamous cell carcinoma and its related complications every year. Therefore, oral squamous cell carcinoma has brought huge challenges and burdens to the entire social medical security system. As a disease caused by multiple factors, current research believes that tobacco and alcohol are important factors leading to its occurrence and development. Similarly, chronic inflammation and human herpes virus infection are also closely...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/5748
Inventor 王月红唐瞻贵刘欧胜邓智元潘灏方小丹全宏志王柏胜顾立群李龙戴小寒
Owner XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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