Establishment and detection method of a liver injury endoplasmic reticulum stress differentially expressed model mouse

Abstract

The invention provides a method for establishing and detecting a liver injury endoplasmic reticulum stress differential expression model mouse, and belongs to the technical field of animal models. BALB / c mice with the same sex, growth age and body weight are divided into 2 groups, the acute group is administered once, the chronic group is administered twice a week, wherein the interval between every two times of administration is 72 hours, and continuous administration is performed for 12-16 weeks; after administration, two groups of mice are sacrificed, and the liver tissue of the mice in thetwo groups is extracted; the expression levels of GRP78 and CHOP in the liver tissue of the mice are measured, and the ratios of CHOP / GRP78 in the two groups are calculated; when the ratio of CHOP / GRP78 in the chronic group is obviously higher than that of the acute group, the mice in the chronic group are the liver injury endoplasmic reticulum stress differential expression model mice. The method for establishing and detecting the liver injury endoplasmic reticulum stress differential expression model mouse has the advantages that a raw material is provided for studying the pathogenesis of acute and chronic liver injury and disease progression, a theoretical basis is provided for the prevention and treatment of clinical liver diseases, the method is simple, the modeling success rate is high, and the repeatability is good.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for establishing and detecting a liver injury endoplasmic reticulum stress differentially expressed model mouse. Background technique [0002] Liver injury is a clinical syndrome of liver function damage caused by a variety of etiologies, characterized by a large number of liver cell degeneration, necrosis, and inflammatory cell infiltration in a short period of time, with high mortality. Hepatocyte necrosis is a common pathological feature of various types of liver injury, including: hepatocyte necrosis, necroptosis, apoptosis and autophagy. The progression of liver disease, that is, liver cell damage is related to various factors. Under the action of various pathogenic factors, liver cells maintain a stable internal environment and promote cell survival by initiating various protective mechanisms. Therefore, the anti-injury ability of liv...

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Problems solved by technology

However, this method does not provide whether the mice treated with the above-mentioned drug administration method really form a mouse liver injury model. It adopts the method of measuring the enzyme activity of AST and ALT in the liver of the drug-administered mice, measuring the liver weight and liver index and doing Pathological sections, it can be seen that although the above scheme discloses the establishment method of the liver injury mouse model, the follow-up verification steps are more complicated, the measurement content is larger, and the judgment method of the measurement results does not give a clear judgment standard. For inexperienced research difficult to judge

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