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Preparation method of controllable double-aqueous-phase droplets based on micro-fluidic technology

A microfluidic technology, a two-phase technology, applied in the field of material chemistry and microfluidic technology, can solve problems such as widespread application obstacles

Inactive Publication Date: 2019-04-19
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are also obvious disadvantages in the two-phase system. Since the surface energy between the two water phases is much smaller than that of the oil-water system, the generation of two-phase droplets, especially the controllable generation, has become an important part of this system in microfluidic liquids. Barriers to widespread adoption of droplet technology

Method used

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  • Preparation method of controllable double-aqueous-phase droplets based on micro-fluidic technology
  • Preparation method of controllable double-aqueous-phase droplets based on micro-fluidic technology
  • Preparation method of controllable double-aqueous-phase droplets based on micro-fluidic technology

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Embodiment 1

[0023] A method for preparing controllable two-phase liquid droplets based on microfluidic technology, characterized in that: comprising the following steps:

[0024] (1) Integration of the pump valve system: using conventional soft lithography method to prepare a PDMS chip, based on a traditional "cross" flow microfluidic droplet chip, integrated on both sides of the dispersed phase channel upstream of the "cross" intersection Pneumatic pump valve; the chip as figure 1 As shown, it is mainly composed of continuous phase inlet 1, gas inlet 2, dispersed phase inlet 3, droplet outlet 4, dispersed phase channel 5, gas channel 6, continuous phase channel 7, main channel 8 and pump valve 9;

[0025] The continuous phase inlet 1 and the dispersed phase inlet 3 are respectively connected to the main channel 8 through the continuous phase channel 7 and the dispersed phase channel 5, and the dispersed phase channel and the continuous phase channel converge to the main channel to form a...

Embodiment 2

[0031] (1) Integration of the pump valve system: using conventional soft lithography method to prepare a PDMS chip, based on a traditional "cross" flow microfluidic droplet chip, integrated on both sides of the dispersed phase channel upstream of the "cross" intersection Pneumatic pump valve; the chip as figure 1 As shown, it is mainly composed of continuous phase inlet 1, gas inlet 2, dispersed phase inlet 3, droplet outlet 4, dispersed phase channel 5, gas channel 6, continuous phase channel 7, main channel 8 and pump valve 9;

[0032] The continuous phase inlet 1 and the dispersed phase inlet 3 are respectively connected to the main channel 8 through the continuous phase channel 7 and the dispersed phase channel 5, and the dispersed phase channel and the continuous phase channel converge to the main channel to form a "cross" intersection; The channel 6 reaches the pneumatic pump valve 9, and the gas in it drives the elastic deformation of the side wall of the pump valve;

...

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Abstract

The invention discloses a preparation method of controllable double-aqueous-phase droplets based on a micro-fluidic technology. The method comprises the steps of integration of a micro-fluidic pump valve system, selection of a double-aqueous-phase system, regulation of droplets and the like. According to the method, the droplets can be controllably stably formed by using the double-aqueous-phase system capable of self-generating phase separation in a cross-shaped flow droplet chip integrated with a pump valve system by adjusting two-phase flow rate, pump valve switching period and the like. The technology is expected to play a role in biological application such as protein separation, cell partition culture, DNA extraction and the like.

Description

technical field [0001] The invention belongs to the fields of microfluidic technology, material chemistry, etc., and particularly relates to a method for preparing controllable two-phase liquid droplets based on microfluidic technology. Background technique [0002] Microfluidic droplet technology is an important branch of microfluidics. It can directly use the incompatibility between fluids to obtain monodisperse droplets and manipulate them for sorting, splitting, capturing, and fusion. Microfluidic droplets have a large specific surface area and can efficiently complete material exchange and chemical reactions in a small volume. Therefore, this technology has been widely used in small molecule detection, single cell analysis, drug delivery, microparticle synthesis, tissue engineering and other fields. [0003] However, the traditional microfluidic droplet technology is based on the oil-water double emulsion system to produce uniform and controllable droplets, which invol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502784
Inventor 秦建华刘海涛魏文博
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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