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Separation and of for component with auxiliary blood fat reducing effect in ganoderma lucidum

A technology for assisting blood lipid lowering and Ganoderma lucidum, applied in the field of natural products, can solve the problems of large liver and kidney toxicity, unstable effect, poor lipid-lowering effect, etc., and achieve the effects of increasing HDL-C content and reducing TC

Inactive Publication Date: 2019-04-23
GUANGZHOU SHENNONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional lipid-lowering drugs are used for treatment. Although they are curative, they need to be taken for a long time to maintain the lipid-lowering effect, which has serious side effects on the liver and kidney.
And when in use, there are still problems of unstable effect and bad lipid-lowering effect
At present, there is no research on how to effectively extract components of Ganoderma lucidum to obtain good auxiliary hypolipidemic products

Method used

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  • Separation and of for component with auxiliary blood fat reducing effect in ganoderma lucidum
  • Separation and of for component with auxiliary blood fat reducing effect in ganoderma lucidum
  • Separation and of for component with auxiliary blood fat reducing effect in ganoderma lucidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 A method for separating components in Ganoderma lucidum with auxiliary blood lipid-lowering effect

[0030] 1) Preparation of ganoderma lucidum alcohol extract:

[0031] Weigh 2000g of Ganoderma lucidum medicinal material, pulverize, add 8 times the weight of 95% ethanol, heat and reflux for extraction for 1 hour, filter, collect the filtrate, add 8 times the weight of 95% ethanol to the filter residue, heat and reflux for 1 hour, filter, and collect the filtrate. The ethanol filtrates extracted twice were combined, concentrated under reduced pressure at 65° C., and dried at 80° C. to obtain 120 g of ethanol extract of Ganoderma lucidum.

[0032] 2) Chromatography of Ganoderma lucidum alcohol extract:

[0033] Weigh 30.0 g of column chromatography silica gel (300-400 mesh) that has been pretreated (dried at 105° C. for 30 min), and pack it into a column by a wet method. The volume of one column is about 8000 ml. Take 100 g of Ganoderma lucidum alcohol extrac...

Embodiment 2

[0038] Embodiment 2 animal auxiliary hypolipidemic model experiment

[0039] Experimental animals: Kunming mice, half male and half female, weighing 18-22 g, were purchased from the Experimental Animal Center of Southern Medical University.

[0040] Reagent test kit:

[0041] Total Cholesterol (TC) Determination Kit (Single Reagent GPO-PAP Method) Nanjing Jiancheng Bioengineering Institute

[0042] Triglyceride (TG) Determination Kit (Single Reagent GPO-PAP Method) Nanjing Jiancheng Bioengineering Institute

[0043] High-density lipoprotein cholesterol (HDL-C) determination kit (two-reagent direct method) Nanjing Jiancheng Institute of Bioengineering High-fat feed preparation: 10% lard, 1% cholesterol, 0.2% sodium cholate, 10% egg yolk powder, 78.8% basal feed.

[0044] Experimental sample:

[0045] Sample I: Ganoderma lucidum alcohol extract, see the product prepared in step 1) of Example 1, that is, the extract before chromatography;

[0046] Sample II: the mixture of N...

Embodiment 3

[0063] Example 3 High-performance liquid chromatographic detection of components in Ganoderma lucidum of the present invention that have auxiliary blood lipid-lowering effects

[0064] High performance liquid chromatography detection method:

[0065] Chromatographic column: TSK gel ODS-80Tm (5μm 150*4.6mm);

[0066] Flow rate: 1.2mL / min;

[0067] Column temperature: 30°C;

[0068] Detection wavelength: 256nm;

[0069] Injection volume: 20μl;

[0070] Mobile phase: ratio in the following table 5, gradient elution.

[0071] Table 5 HPLC method mobile phase elution ratio

[0072] step

time (min)

0.1% trifluoroacetic acid acetonitrile (%)

0.1% trifluoroacetic acid water (%)

1

0

26

74

2

35

26

74

3

45

30

70

4

65

38

62

5

90

65

35

6

120

100

0

[0073] Preparation method of reference solution: Weigh 5 mg of Ganoderma acid A, Ganoderma acid B, Ganoderma acid C2, Gano...

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Abstract

The invention discloses a separation method and application of a component with an auxiliary blood fat reducing effect in ganoderma lucidum. The separation method comprises the steps that alcohol extraction is conducted on the ganoderma lucidum, and obtained ganoderma lucidum alcohol extracts are eluted through specific chromatography to obtain the component with the auxiliary blood fat reducing effect. The component with the auxiliary blood fat reducing effect can significantly inhibit the content of TC and TG of mice, and the content of HDL-C is significantly increased. The component which is obtained after separation and purification and has the auxiliary blood fat reducing effect is obviously superior to the alcohol extracts without separation and purification or other purified collected samples in blood fat reducing.

Description

technical field [0001] The invention relates to a method for extracting and isolating components with an auxiliary blood lipid-lowering effect from Ganoderma lucidum and an application thereof, belonging to the field of natural products. Background technique [0002] Blood lipids are the general term for neutral fats (triglycerides) and lipids (phospholipids, glycolipids, sterols, steroids) in plasma, which are widely present in the human body. They are essential substances for the basic metabolism of living cells. Generally speaking, the main components of blood lipids are triglycerides and cholesterol, among which triglycerides participate in energy metabolism in the human body, while cholesterol is mainly used for the synthesis of cell membranes, steroid hormones and bile acids. [0003] With the rise of the economic level, people's living standards have also gradually improved, and the daily intake of protein, calories, fat and other components has increased, coupled wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61P3/06A23L33/105
CPCA61K36/074A23L33/105A23V2002/00A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61P3/06A23V2200/3262A23V2250/208
Inventor 李莹雪李乾坤丁韵芸王锐银黄静贤李紫薇
Owner GUANGZHOU SHENNONG BIOLOGICAL TECH
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