A method for preparing harmful heavy metal adsorbent by using active polysaccharide of Corkia bacterium

A technology of Corkella and active polysaccharide, applied in the field of water pollution treatment, can solve the problems of unsatisfactory effect of heavy metal adsorption and removal, etc.

Active Publication Date: 2021-09-17
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In order to solve the problem of unsatisfactory adsorption and removal of harmful heavy metals in existing tap water, the present invention provides a method for preparing harmful heavy metal adsorbents by using active polysaccharides from Cochlear bacteria

Method used

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  • A method for preparing harmful heavy metal adsorbent by using active polysaccharide of Corkia bacterium

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Embodiment 1

[0065] A method for preparing a harmful heavy metal adsorbent by using the active polysaccharide of Corkia bacterium, said method comprising the following steps:

[0066] 1) Cultivate strains: Prepare 2.7L of microbial culture fluid, which contains the following substances in weight percentage: 5% potato juice, 1% clam broth, 0.06% dipotassium hydrogen phosphate and 2% glucose, and the balance is aged Seawater: Inoculate the selected Cochlear bacteria into the microbial culture solution to form a bacterial solution, and cultivate the selected strains of the bacterial solution. The cultivation conditions are: in an environment of 30°C, cultivated on a shaker at 150r / min for 3d ;

[0067] 2) Extraction of flocculant: Concentrate the cultivated bacterial liquid, concentrate the bacterial liquid to 12% of the original volume during the concentration process, put the concentrated bacterial liquid into a 2000 Dalton dialysis bag, dialyze for 60 hours, and then dialyze The final ret...

Embodiment 2

[0070] A method for preparing a harmful heavy metal adsorbent by using the active polysaccharide of Corkia bacterium, said method comprising the following steps:

[0071] 1) Cultivate strains: prepare 3.0L of microbial culture fluid, which contains the following substances in weight percent: 8% potato juice, 3% clam broth, 0.18% dipotassium hydrogen phosphate and 3% glucose, and the balance is aged Inoculate the seawater; inoculate the selected Cochlear bacteria into the microbial culture solution to form a bacterial solution, and cultivate the selected strains of the bacterial solution. The cultivation conditions are: in an environment of 25°C, cultivate on a shaking table at 200r / min 5d;

[0072] 2) Extraction of flocculant: Concentrate the cultivated bacterial liquid, concentrate the bacterial liquid to 9% of the original volume during the concentration process, put the concentrated bacterial liquid into a 5000 Dalton dialysis bag, dialyze for 36 hours, and then dialyze Th...

Embodiment 3

[0075] A method for preparing a harmful heavy metal adsorbent by using the active polysaccharide of Corkia bacterium, said method comprising the following steps:

[0076] 1) Cultivate bacterial strains: prepare 2.7L of microbial culture solution, which contains the following substances in weight percentage: 6.5% of potato juice, 1.5% of clam broth, 0.12% of dipotassium hydrogen phosphate and 2.25% of glucose, and the balance is old Thin seawater; Inoculate the selected Cochlear bacteria into the microbial culture solution to form a bacterial solution, and cultivate the selected strains of the bacterial solution. The cultivation conditions are: in an environment of 27°C, cultivate on a shaking table at 180r / min 4d;

[0077] 2) Extraction of flocculant: Concentrate the cultivated bacterial liquid, concentrate the bacterial liquid to 300mL during the concentration process, put the concentrated bacterial liquid into a 2000 Dalton dialysis bag, dialyze for 48 hours, and then remove...

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Abstract

The invention relates to the technical field of water pollution treatment. In order to solve the problem of unsatisfactory adsorption and removal of heavy metals in existing tap water, the invention provides a method for preparing a harmful heavy metal adsorbent by using the active polysaccharide of Cochlear bacteria. The method comprises the following steps: 1) cultivating bacterial strains: inoculating selected Cochlear bacteria into microbial culture liquid to form bacterial liquid, and cultivating the selected bacterial strains in the bacterial liquid; Concentration, dialysis and centrifugation are carried out to extract the microbial flocculant; 3) preparation of adsorbent: the microbial flocculant is dissolved in the mixed aqueous solution of agar and starch, and the harmful heavy metal adsorbent is obtained after solidification. The overall steps of the method of the present invention are simple, and the preparation efficiency and yield are high; the prepared adsorbent has the advantages of being safe and harmless, and can be directly added to drinking water to realize the adsorption and removal of harmful heavy metal components, and has no harmful effects on heavy metals. High component adsorption efficiency and good adsorption effect.

Description

technical field [0001] The invention relates to the technical field of water pollution treatment, in particular to a method for preparing a harmful heavy metal adsorbent by utilizing the active polysaccharide of Cochlear bacteria. Background technique [0002] Most of the water used by people in production and life is tap water, which is mainly obtained by treating water drawn from groundwater or river water by water plants. Because some water sources are affected by industrial or domestic sewage discharges, the heavy metal content exceeds the standard. Although it is treated by a water plant before being used for human and livestock consumption, the content of some heavy metals is still at a high level, and some even exceed the standard; in addition, urban tap water pipelines Old age will also lead to the infiltration of heavy metals such as lead into the water body, causing the heavy metal content to exceed the standard. [0003] As Singapore Lianhe Zaobao.com reported on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/30B01J20/24C02F1/28C02F101/20
CPCB01J20/24B01J2220/4825C02F1/286C02F2101/20C02F2101/206
Inventor 穆军
Owner ZHEJIANG OCEAN UNIV
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