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Method for preparing diglyceride by using high acid value oil as raw material

A high-acid value oil and diglyceride technology, which is applied in fermentation and other directions, can solve problems such as complex conditions, and achieve the effect of increasing oil yield, increasing yield, and high diglyceride content

Active Publication Date: 2019-04-23
脂代生物科技(杭州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although enzymatic glycerol hydrolysis of glycerol and triglycerides has been reported to yield appreciable diglyceride content, the situation is more complicated for oils with high acid value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 30 parts by mass of soybean oil with an acid value of 40KOH / (mg / g) and a triglyceride content of 79.9wt% were mixed evenly with 3.4 parts by mass of glycerin, and then 1.5 parts by mass of immobilized enzyme Novozym 435 (purchased from Novozymes company) was added and 5 parts by mass Molecular sieve, and then stirred and reacted at 65° C. and 500 rpm for 8 hours to obtain the product.

[0029] The composition of the product was analyzed by HPLC-ELSD. The content of free fatty acid in the product obtained in this example was 0.28wt%, and the content of diglyceride was 61.8wt%.

Embodiment 2

[0031] 50 parts by mass of rapeseed oil with an acid value of 50KOH / (mg / g) and a triglyceride content of 74.9 wt% were mixed with 4.1 parts by mass of glycerin, and then 2.6 parts by mass of immobilized enzyme Novozym 435 (purchased from Novozymes company ), and then stirred and reacted for 10 hours at 70° C., 500 rpm and under vacuum conditions to obtain the product.

[0032] The composition of the product was analyzed by HPLC-ELSD. The content of free fatty acid in the product obtained in this example was 0.22wt%, and the content of diglyceride was 68.1wt%.

Embodiment 3

[0034] 40 parts by mass of lard with an acid value of 60KOH / (mg / g) and a triglyceride content of 69.8wt% were mixed uniformly with 5.6 parts by mass of glycerin, and then 2.6 parts by mass of immobilized enzyme NS40086 (purchased from Novozymes Company) were added. Pass in an inert gas, then stir the reaction at 55° C. and 450 rpm for 15 hours to obtain the product.

[0035] The composition of the product was analyzed by HPLC-ELSD. The content of free fatty acid in the product obtained in this example was 0.17wt%, and the content of diglyceride was 64.3wt%.

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PUM

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Abstract

The invention discloses a method for preparing diglyceride by using high acid value oil as a raw material. The method comprises the following step of making the oil react with glycerol under lipase catalysis to obtain a product with a low free fatty acid content and a high diglyceride content; the acid value of the oil is equal to or less than 80 KOH / (mg / g), the triglyceride content in the oil isequal to or greater than 59.8 wt%, the content of the diglyceride in a reaction product is 59-68wt%, and the content of the free fatty acid is equal to or less than 0.30wt%. The method adopts the highacid value oil as a raw material, has abundant raw material sources, converts free fatty acid into neutral glyceride, effectively reduces the content of the free fatty acid in oil, has the double effects of deacidification and diglyceride content increasing, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of glyceride synthesis and processing, and in particular relates to a method for preparing diglyceride from oil with high acid value. Background technique [0002] Diglycerides are a nutritious and healthy oil that has a similar taste and flavor to regular oils. Diacylglycerol includes 1,3-diglyceride and 1,2(2,3)-diglyceride, of which 1,3-diglyceride is relatively stable and accounts for the main component. The metabolic pathway of 1,3-diglyceride is different from that of ordinary triglyceride. 1,3-diglyceride is ingested by the human body and processed by digestive enzymes to generate glycerol and free fatty acids, which are converted into energy in the body. Therefore, the intake of diglycerides can reduce the accumulation of fat in the body, has the effect of losing weight, and can prevent obesity-related diseases such as diabetes. The research and development of diglyceride has a good application pro...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6454
Inventor 钟南京陈泓晓杨坤鹏曾瑶英林琳
Owner 脂代生物科技(杭州)有限公司