Vitrification freezing reagent and vitrification unfreezing reagent as well as application and use method thereof

A vitrification freezing and reagent technology, which is applied in biochemical equipment and methods, applications, cell culture active agents, etc., can solve the problem of no domestic reagents, and achieve the effect of reasonable solute concentration, avoiding errors, and simplifying the operation process.

Inactive Publication Date: 2019-04-26
白晓红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The consumable reagents used in the domestic medical industry implement the medical registration certificate system. At present, the “certified” reagents for embryo freezing and embryo thawing and recovery in the domestic assisted pregnancy industry are only imported reagents (Kato’s reagents in Japan), and there are no domestic reagents.

Method used

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  • Vitrification freezing reagent and vitrification unfreezing reagent as well as application and use method thereof
  • Vitrification freezing reagent and vitrification unfreezing reagent as well as application and use method thereof
  • Vitrification freezing reagent and vitrification unfreezing reagent as well as application and use method thereof

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Effect test

Embodiment Construction

[0043] This patented product includes two parts: vitrification freezing reagent and vitrification thawing reagent.

[0044] Reagent formula for vitrification:

[0045]

[0046] D-PBS formula (1000ml):

[0047] Sodium chloride 8.0g

[0048] Potassium chloride 0.2g

[0049] Disodium hydrogen phosphate 1.15g

[0050] Potassium dihydrogen phosphate 0.2g

[0051] Calcium chloride 0.1g

[0052] Magnesium chloride 0.1g

[0053] Glucose 1.0g

[0054] Sodium pyruvate 36mg

[0055] Reagent formula for vitrification and thawing:

[0056]

[0057]D-PBS formula (1000ml):

[0058] Sodium chloride 8.0g

[0059] Potassium chloride 0.2g

[0060] Disodium hydrogen phosphate 1.15g

[0061] Potassium dihydrogen phosphate 0.2g

[0062] Calcium chloride 0.1g

[0063] Magnesium chloride 0.1g

[0064] Glucose 1.0g

[0065] Sodium pyruvate 36mg

[0066] ⑴ Human cleavage-stage embryo vitrification reagents and methods of use

[0067] Structure and composition: This product inclu...

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Abstract

The invention relates to the technical field of freezing and unfreezing and in particular relates to a vitrification freezing reagent and a vitrification unfreezing reagent as well as application anda use method thereof. The invention provides the vitrification freezing reagent and the vitrification unfreezing reagent which are short in freezing time and unfreezing time, convenient to operate andcapable of preventing embryo damage. Compared with a current imported reagent, the freezing and unfreezing method provided by the invention has the benefits that (1) both the freezing time and the unfreezing time are shortened, and the working efficiency is improved; (2) freezing reagent solute has a reasonable concentration, an embryo can be easily dissociated from a freezing carrier, and excessive operation and damage to the embryo are avoided; (3) an unfreezing liquid has a working temperature of 37 DEG C, the unfreezing process can be continuously carried out on a working table, and the working table does not need to be replaced, so that operation procedures are simplified and errors are avoided.

Description

technical field [0001] The invention relates to the technical field of freezing and thawing, in particular to a vitrification freezing reagent and a vitrification thawing reagent and their application and usage method. Background technique [0002] With the increase of infertile population, the opening of the second-child policy and the development of assisted reproductive technology, the amount of IVF treatment (number of cycles) in my country is increasing year by year. According to the development of assisted reproductive technology in my country, academician Qiao Jie, chairman of the Reproductive Medicine Professional Committee of the Chinese Medical Doctor Association, predicts that the amount of IVF treatment (number of cycles) in China will reach 400,000 cases per year. [0003] Embryo cryopreservation and thawing recovery technology is an important technology in the field of human assisted reproductive technology (in vitro fertilization and embryo transfer technology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/073
CPCA01N1/0221C12N5/0604C12N2500/12C12N2500/14C12N2500/16C12N2500/30C12N2500/34C12N2500/35C12N2500/62C12N2501/998
Inventor 白晓红
Owner 白晓红
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