Strain for stably and efficiently hydrolyzing oil meal and application of strain

A technology of oilseed meal and bacterial strains, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of uncontrollable process, long fermentation cycle, cumbersome steps, etc., achieve strong adaptability and tolerance, reduce fermentation cycle, The effect of accelerating the fermentation speed

Active Publication Date: 2019-04-26
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, the method of hydrolyzing oilseed meal at home and abroad is mainly to use mold to carry out solid fermentation, and then carry out shock hydrolysis at an appropriate temperature. Stability and other shortcomings greatly limit the industrialization and application of oilseed meal hydrolyzate

Method used

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  • Strain for stably and efficiently hydrolyzing oil meal and application of strain
  • Strain for stably and efficiently hydrolyzing oil meal and application of strain
  • Strain for stably and efficiently hydrolyzing oil meal and application of strain

Examples

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Effect test

Embodiment 1

[0026] A method for stably and efficiently hydrolyzing oilseed meal, the method specifically comprising: mixing oilseed meal, glucose and water in proportion, sterilizing at 121°C for 20 minutes, and adding hydrolyzate to it at a ratio of 5% of the volume of the fermentation broth after cooling. Strain CX-20 was then stirred and fermented at 28° C. for 72 hours in an air-lift fermenter without feeding or changing fermentation conditions to obtain oilseed meal hydrolyzate.

[0027] In this embodiment, rapeseed meal, soybean meal and peanut meal were used for the test respectively; wherein, for each oil seed meal, the concentration in water was within the range of 30-150g / L, and 30, 60, 90, 120, 150g / L were tested; the added concentration of glucose in water was 20g / L. As the concentration of oilseed meal increases, the hydrolysis time will gradually increase, and the addition of carbon source can promote the growth of Bacillus amyloliquefaciens, thereby shortening the fermentat...

Embodiment 2

[0031]A method for stably and efficiently hydrolyzing oilseed meal, the method specifically comprising: mixing oilseed meal, glucose and water in proportion, sterilizing at 121°C for 20 minutes, and adding hydrolyzate to it at a ratio of 5% of the volume of the fermentation broth after cooling. Bacillus amylogenus CX-20 is then stirred and fermented at 28° C. for 72 hours in an air-lift fermenter without feeding or changing fermentation conditions to obtain oilseed meal hydrolyzate.

[0032] In this example, the effect of adding different concentrations of carbon sources on the composition of oilseed meal hydrolyzate was studied. The oilseed meal was tested with rapeseed meal, soybean meal and peanut meal respectively; the concentration of each oilseed meal in water was within the range of 90 g / L; the concentration of glucose in water was 0-100g / L g / L L, choose 0, 20, 40, 60, 80, 100g / L respectively for testing.

[0033] Under the condition of adding different initial concent...

Embodiment 3

[0037] A method for stably and efficiently hydrolyzing rapeseed meal, the method specifically comprising: mixing rapeseed meal, glucose and water in proportion, sterilizing at 121°C for 20 minutes, and adding hydrolyzate to it at a ratio of 5% of the volume of the fermentation broth after cooling. Bacillus amylogenus CX-20 was then stirred and fermented at 28° C. for 72 hours in an air-lift fermenter without feeding or changing fermentation conditions to obtain oilseed meal hydrolyzate. Among them, the initial concentration of rapeseed meal was 90 g / L, and the initial concentration of glucose was 80 g / L.

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Abstract

The invention belongs to the field of microbial fermentation and specifically relates to a strain for stably and efficiently hydrolyzing oil meal and application of the strain. The strain is preservedin China Center for Type Culture Collection called CCTCC for short on November 14, 2018; a preservation address is Wuhan University, Wuhan, China; and a preservation number is CCTCC NO: M 2018794. According to the strain provided by the invention, the oil meal is stably and efficiently hydrolyzed through liquid fermentation; the time is short, steps are simple and a process is controllable and monitored; and meanwhile, composing components of generated hydrolysis fluid are more stable and the strain is applicable to market requirements and subsequent application.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a bacterial strain for stably and efficiently hydrolyzing oilseed meal and its application. Background technique [0002] For many years, my country has always ranked first in the world in the cultivation and processing of grain and oil crops. Oilseed meal resources are not only diverse in variety and rich in yield, but also rich in various nutrients, especially the protein content is about 40% to 50%, and it also contains 2 to 3% starch, 7 to 8% sucrose and a certain amount of residual Oil is also a good source of various beneficial trace elements such as phosphorus, calcium, magnesium, copper, zinc, and manganese. However, in traditional agriculture, due to the existence of some anti-nutritional factors and the physical and chemical structure of the interaction of different substances, the utilization of nutrients by plants or animals is greatly limited, such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/07
CPCC12P1/04C12N1/205C12R2001/07
Inventor 陈文超万霞李旋马旭丽黄凤洪
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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